BioVector? NCTC 8241 (Acinetobacter calcoaceticus) 不動(dòng)桿菌菌株 / BioVector? NCTC 8241 (Acinetobacter calcoaceticus) Strain

通用定義 / General Definition:BioVector? NCTC 8241 是不動(dòng)桿菌屬 (Acinetobacter) 的模式菌株，分類學(xué)上屬于 不動(dòng)桿菌 (Acinetobacter calcoaceticus)。不動(dòng)桿菌屬是一類革蘭氏陰性、不發(fā)酵糖類、非動(dòng)力性的球桿菌，廣泛分布于土壤和水體中。NCTC 8241 菌株作為該屬的典型代表，被廣泛用于微生物分類學(xué)鑒定、抗生素耐藥性機(jī)制研究以及生物修復(fù)（如降解碳?xì)浠衔铮┑阮I(lǐng)域。它是區(qū)分不動(dòng)桿菌屬內(nèi)不同物種（特別是與臨床相關(guān)的鮑曼不動(dòng)桿菌復(fù)合物）的重要參考標(biāo)準(zhǔn)。

BioVector? NCTC 8241 is a type strain of the genus Acinetobacter, taxonomically identified as Acinetobacter calcoaceticus. Members of the Acinetobacter genus are Gram-negative, non-fermentative, non-motile coccobacilli that are ubiquitous in soil and water environments. As a representative strain, NCTC 8241 is extensively used in microbial taxonomy, studies of antibiotic resistance mechanisms, and bioremediation (e.g., degradation of hydrocarbons). It serves as a fundamental reference standard for distinguishing species within the genus, particularly in differentiating it from the clinically significant A. baumannii complex.

BioVector? NCTC 8241 技術(shù)說明書 (Technical Datasheet)

中文版說明書 (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? NCTC 8241 (Acinetobacter calcoaceticus) 菌株

• 其他編號： ATCC 23055, DSM 30006

• 生物分類： 不動(dòng)桿菌屬 (Acinetobacter)

• 形態(tài)特征： 革蘭氏陰性球桿菌，成對或鏈狀排列；在對數(shù)生長期呈桿狀，在穩(wěn)定期趨于球狀。

2. 培養(yǎng)條件

• 培養(yǎng)基： BioVector? 營養(yǎng)肉湯 (Nutrient Broth) 或 胰蛋白酶大豆瓊脂 (TSA)。

• 培養(yǎng)溫度： 最適生長溫度為 30 攝氏度（注意：許多 A. calcoaceticus 菌株在 37 攝氏度下生長較弱，這與鮑曼不動(dòng)桿菌不同）。

• 培養(yǎng)環(huán)境： 專性需氧，建議搖床轉(zhuǎn)速為 200 rpm。

• 生長特性： 菌落通常呈圓形、光滑、凸起，邊緣整齊。

3. 菌株應(yīng)用

• 分類與鑒定： 作為不動(dòng)桿菌屬的指紋圖譜和基因組比對的標(biāo)準(zhǔn)參考菌株。

• 生物修復(fù)： 研究其降解原油、酚類化合物及其他有機(jī)污染物的代謝途徑。

• 抗藥性研究： 比較環(huán)境菌株與臨床菌株在耐藥基因獲取和外排泵機(jī)制上的差異。

4. 注意事項(xiàng)

• 溫度敏感性： 嚴(yán)格遵守 30°C 的培養(yǎng)建議，高溫可能導(dǎo)致生長受限。

• 安全性： 歸類為生物安全一級 (BSL-1)。雖然通常被認(rèn)為對健康人類無致病性，但在處理時(shí)仍需遵循標(biāo)準(zhǔn)實(shí)驗(yàn)室無菌操作。

English Datasheet

1. General Product Information

• Product Name: BioVector? NCTC 8241 (Acinetobacter calcoaceticus) Strain

• Alternative Designations: ATCC 23055, DSM 30006

• Biological Classification: Genus Acinetobacter

• Morphology: Gram-negative coccobacilli, appearing in pairs or chains; typically rod-shaped during log phase and more coccoid during stationary phase.

2. Culture Conditions

• Media: BioVector? Nutrient Broth or Tryptic Soy Agar (TSA).

• Incubation Temperature: Optimal growth at 30°C. (Note: Unlike A. baumannii, many A. calcoaceticus strains grow poorly or not at all at 37°C).

• Environment: Obligately aerobic; recommended shaking at 200 rpm for liquid cultures.

• Growth Characteristics: Colonies are generally circular, smooth, convex, and entire.

3. Applications

• Taxonomy and Diagnostics: Serving as the definitive reference strain for genomic comparisons and biochemical profiling within the Acinetobacter genus.

• Environmental Bioremediation: Investigating metabolic pathways for the degradation of crude oil, phenols, and other complex hydrocarbons.

• Comparative Genomics: Studying the evolution of antibiotic resistance by comparing environmental type strains with clinical isolates.

4. Key Usage Notes

• Thermal Constraints: Adherence to the 30°C incubation temperature is critical for consistent growth and phenotypic expression.

• Safety Protocols: Generally categorized as Biosafety Level 1 (BSL-1). While typically non-pathogenic to healthy individuals, standard aseptic laboratory techniques should be maintained.

注意 / Note: NCTC 8241 的天然遺傳轉(zhuǎn)化能力是其重要特征。BioVector? 建議在進(jìn)行基因重組實(shí)驗(yàn)時(shí)，利用其高效率的自然轉(zhuǎn)化特性來構(gòu)建突變體。A key feature of NCTC 8241 is its natural genetic competence. BioVector? recommends leveraging its high efficiency for natural transformation when constructing mutants or performing gene recombination experiments.

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