BioVector? Butyricimonas virosa 丁酸單胞菌屬菌株 / BioVector? Butyricimonas virosa Strain

通用定義 / General Definition:

BioVector? Butyricimonas virosa 是一種屬于擬桿菌目 (Bacteroidales)、臭氣桿菌科 (Odoribacteraceae) 的革蘭氏陰性、專性厭氧、不產(chǎn)芽孢桿菌。該菌株最初從人類糞便樣本中分離得到。其最顯著的代謝特征是能夠產(chǎn)生大量的丁酸 (Butyrate)，這是一種對(duì)腸道健康至關(guān)重要的短鏈脂肪酸 (SCFA)。B. virosa 被認(rèn)為是腸道微生態(tài)系統(tǒng)中的重要成員，在維持免疫穩(wěn)態(tài)、抗炎作用以及調(diào)節(jié)宿主代謝（如葡萄糖穩(wěn)態(tài)）中發(fā)揮著潛在的關(guān)鍵作用。

BioVector? Butyricimonas virosa is a Gram-negative, obligately anaerobic, non-spore-forming rod-shaped bacterium belonging to the order Bacteroidales and the family Odoribacteraceae. Originally isolated from human faecal samples, its most significant metabolic hallmark is the production of substantial amounts of butyrate, a short-chain fatty acid (SCFA) vital for intestinal health. B. virosa is recognized as an important commensal in the gut microbiome, playing potential roles in maintaining immune homeostasis, exerting anti-inflammatory effects, and regulating host metabolism (e.g., glucose homeostasis).

BioVector? B. virosa 技術(shù)說(shuō)明書 (Technical Datasheet)

中文版說(shuō)明書 (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? Butyricimonas virosa 菌株

• 生物分類： 丁酸單胞菌屬 (Butyricimonas)

• 基因組特征： 含有參與碳水化合物發(fā)酵產(chǎn)生丁酸的完整酶系（如丁酸激酶通路）。

• 形態(tài)特征： 短桿狀，通常長(zhǎng) 0.5–1.0 $\mu$m。

2. 培養(yǎng)條件

• 培養(yǎng)基： BioVector? 加強(qiáng)梭菌培養(yǎng)基 (RCM) 或 腦心浸液培養(yǎng)基 (BHI)，建議添加 5% 羊血或血紅素/維生素 K1。

• 培養(yǎng)溫度： 37 攝氏度。

• 厭氧環(huán)境： 必須在嚴(yán)格厭氧條件下培養(yǎng)（$N_2$ 80%, $CO_2$ 10%, $H_2$ 10%）。

• 生長(zhǎng)特性： 在瓊脂平板上形成圓形、半透明至白色的微小菌落。

3. 菌株應(yīng)用

• 代謝組學(xué)研究： 作為丁酸產(chǎn)生菌模型，研究短鏈脂肪酸對(duì)腸道上皮屏障功能的增強(qiáng)作用。

• 疾病關(guān)聯(lián)研究： 探討該菌在肥胖、2型糖尿病及炎癥性腸病 (IBD) 患者腸道中的豐度變化及其生物學(xué)意義。

• 益生元篩選： 評(píng)估不同膳食纖維（如菊粉、抗性淀粉）對(duì) B. virosa 生長(zhǎng)及產(chǎn)酸效率的促進(jìn)作用。

4. 注意事項(xiàng)

• 氧敏感性： 該菌對(duì)氧氣極其敏感，接種及操作過(guò)程需在厭氧工作站內(nèi)完成，暴露于空氣中會(huì)導(dǎo)致活力迅速喪失。

• 生物安全： 通常歸類為生物安全一級(jí) (BSL-1)，但處理來(lái)自臨床樣本的變體時(shí)應(yīng)遵循 BSL-2 規(guī)范。

English Datasheet

1. General Product Information

• Product Name: BioVector? Butyricimonas virosa Strain

• Biological Classification: Genus Butyricimonas

• Genomic Features: Possesses a complete set of enzymes for carbohydrate fermentation leading to butyrate (e.g., butyrate kinase pathway).

• Morphology: Short rods, typically 0.5–1.0 $\mu$m in length.

2. Culture Conditions

• Media: BioVector? Reinforced Clostridial Medium (RCM) or Brain Heart Infusion (BHI) broth/agar, supplemented with 5% sheep blood or hemin/vitamin K1.

• Incubation Temperature: 37°C.

• Anaerobic Environment: Must be cultured under strictly anaerobic conditions ($N_2$ 80%, $CO_2$ 10%, $H_2$ 10%).

• Growth Characteristics: Forms small, circular, translucent to white colonies on anaerobic blood agar.

3. Applications

• Metabolomics: Serving as a model for butyrate-producing bacteria to study the enhancement of intestinal barrier integrity by SCFAs.

• Microbiome Association Studies: Investigating the correlation between B. virosa abundance and metabolic disorders such as obesity, Type 2 Diabetes, and Inflammatory Bowel Disease (IBD).

• Prebiotic Screening: Evaluating the fermentation efficiency of various dietary fibers (e.g., inulin, resistant starch) by this strain.

4. Key Usage Notes

• Oxygen Sensitivity: This strain is highly sensitive to oxygen. All manipulations, including inoculation and subculturing, should be performed inside an anaerobic chamber to prevent loss of viability.

• Storage: Long-term storage requires cryopreservation in anaerobic glycerol stock at -80°C or lyophilization.

注意 / Note:Butyricimonas virosa 在腸道中的減少往往與代謝炎癥相關(guān)。BioVector? 建議在共培養(yǎng)實(shí)驗(yàn)中使用專門設(shè)計(jì)的厭氧培養(yǎng)基，以模擬腸道內(nèi)復(fù)雜的營(yíng)養(yǎng)競(jìng)爭(zhēng)環(huán)境。

The depletion of Butyricimonas virosa in the gut is often associated with metabolic inflammation. BioVector? recommends using specialized anaerobic media in co-culture experiments to simulate the complex nutritional competition within the intestinal environment.

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