BioVector? MH-S 小鼠肺泡巨噬細(xì)胞系 / BioVector? MH-S Mouse Alveolar Macrophage Cell Line

通用定義 / General Definition:

BioVector? MH-S 是一種源自 BALB/c 小鼠的永生化肺泡巨噬細(xì)胞系。該細(xì)胞系通過 SV40 病毒轉(zhuǎn)化建立，保留了肺泡巨噬細(xì)胞的關(guān)鍵生理特征。MH-S 細(xì)胞具有活躍的吞噬能力，能夠產(chǎn)生細(xì)胞因子，并表達(dá)巨噬細(xì)胞特異性表面標(biāo)志物。作為研究肺部免疫學(xué)、呼吸道感染、哮喘、肺纖維化以及環(huán)境顆粒物（如 PM2.5）毒理學(xué)的標(biāo)準(zhǔn)模型，MH-S 提供了比原代細(xì)胞更高的實(shí)驗(yàn)重現(xiàn)性和操作便捷性。

BioVector? MH-S is an immortalized alveolar macrophage cell line derived from BALB/c mice. Established via SV40 transformation, the cell line retains the essential physiological characteristics of primary alveolar macrophages. MH-S cells exhibit active phagocytic capacity, produce various cytokines, and express macrophage-specific surface markers. As a standard model for studying pulmonary immunology, respiratory infections, asthma, lung fibrosis, and the toxicology of environmental particulates (e.g., PM2.5), MH-S offers superior experimental reproducibility and ease of handling compared to primary cells.

BioVector? MH-S 技術(shù)說明書 (Technical Datasheet)

中文版說明書 (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? MH-S 小鼠肺泡巨噬細(xì)胞

• 組織來源： 肺 (Lung/Alveoli)

• 生長特性： 貼壁生長（有時(shí)伴有少量懸浮細(xì)胞）

• 細(xì)胞形態(tài)： 巨噬細(xì)胞樣，呈圓形或多角形，具有典型的偽足結(jié)構(gòu)。

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? RPMI-1640 培養(yǎng)基。

• 血清添加： 10% BioVector? 優(yōu)質(zhì)胎牛血清 (FBS)。

• 關(guān)鍵添加劑： 需添加 0.05 mM $\beta$-巰基乙醇 ($\beta$-mercaptoethanol) 以維持細(xì)胞活性。

• 培養(yǎng)環(huán)境： 37 攝氏度，5% $CO_2$。

• 傳代比例： 1:2 至 1:4；建議使用細(xì)胞刮刀（Scraper）或輕微胰酶消化進(jìn)行傳代，避免過度消化損傷膜受體。

3. 細(xì)胞應(yīng)用

• 呼吸道感染研究： 研究肺部病原體（如結(jié)核桿菌、流感病毒）與巨噬細(xì)胞的相互作用。

• 炎癥反應(yīng)： 評(píng)估 LPS 或其他刺激物誘導(dǎo)的促炎細(xì)胞因子（TNF-$\alpha$, IL-6）釋放。

• 吞噬實(shí)驗(yàn)： 定量檢測細(xì)胞對(duì)熒光標(biāo)記細(xì)菌或環(huán)境塵埃顆粒的吞噬效率。

4. 注意事項(xiàng)

• 貼壁性： MH-S 細(xì)胞貼壁較牢固，但在生長過快或營養(yǎng)不足時(shí)可能出現(xiàn)部分脫落懸浮，這屬于正常現(xiàn)象。

• 激活狀態(tài)： 巨噬細(xì)胞對(duì)培養(yǎng)基中的內(nèi)毒素非常敏感，BioVector? 建議使用極低內(nèi)毒素含量的 FBS。

English Datasheet

1. General Product Information

• Product Name: BioVector? MH-S Mouse Alveolar Macrophage Cell Line

• Tissue Source: Lung (Alveoli)

• Growth Properties: Adherent (occasionally with some cells in suspension)

• Morphology: Macrophage-like; round or polygonal with characteristic pseudopodia.

2. Culture Conditions

• Basal Medium: BioVector? RPMI-1640 Medium.

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS).

• Key Supplements: Supplemented with 0.05 mM $\beta$-mercaptoethanol to maintain cellular redox balance and viability.

• Incubation: 37 degrees Celsius, 5% $CO_2$.

• Subculturing: 1:2 to 1:4 ratio; use a cell scraper or gentle trypsinization. Avoid prolonged enzymatic treatment to preserve membrane-bound receptors.

3. Applications

• Respiratory Infection Modeling: Investigating the interaction between pulmonary pathogens (e.g., M. tuberculosis, Influenza virus) and host immune cells.

• Inflammatory Response: Measuring the release of pro-inflammatory cytokines (TNF-$\alpha$, IL-6) induced by LPS or other irritants.

• Phagocytosis Assays: Quantifying the uptake efficiency of fluorescently labeled bacteria or environmental particulate matter.


Macrophage Anatomy. Structure Of Immune Cell. Process Phagocytosis Of A Bacterium With Formation Of

4. Key Usage Notes

• Adhesion Profile: Healthy MH-S cells form a stable monolayer, but partial detachment may occur during rapid proliferation or nutrient depletion.

• Activation Sensitivity: Macrophages are highly reactive to endotoxins. BioVector? recommends the use of ultra-low endotoxin FBS and certified pyrogen-free plasticware to prevent baseline activation.

注意 / Note: BioVector? MH-S 應(yīng)在生物安全二級(jí) (BSL-2) 條件下操作。該細(xì)胞系是替代原代支氣管肺泡灌洗 (BAL) 細(xì)胞的高效工具，非常適合高通量篩選實(shí)驗(yàn)。

BioVector? MH-S should be handled under Biosafety Level 2 (BSL-2). This cell line is a highly efficient alternative to primary Bronchoalveolar Lavage (BAL) cells and is particularly well-suited for high-throughput screening assays.

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