BioVector? Pantoea ananatis PA13 歐文氏菌科泛菌屬菌株 / BioVector? Pantoea ananatis PA13 Strain

通用定義 / General Definition:BioVector? Pantoea ananatis PA13 是一種多功能的革蘭氏陰性、非芽孢桿菌。該菌株屬于歐文氏菌科（Erwiniaceae），最初因其在多種農(nóng)作物（如菠蘿、洋蔥、水稻）中作為致病菌或共生菌而被廣泛研究。然而，PA13 菌株在合成生物學(xué)和代謝工程領(lǐng)域表現(xiàn)出極大的潛力，特別是其具有高效的生物質(zhì)轉(zhuǎn)化能力和對多種環(huán)境壓力（如高滲透壓和溫差）的強(qiáng)耐受性。PA13 常被用作生產(chǎn)高附加值化學(xué)品（如類胡蘿卜素、氨基酸）的底盤細(xì)胞，以及研究植物-微生物相互作用的遺傳學(xué)模型。

BioVector? Pantoea ananatis PA13 is a versatile Gram-negative, non-spore-forming bacterium. Belonging to the family Erwiniaceae, this strain was initially studied for its role as either a pathogen or a symbiont in various crops (e.g., pineapple, onion, and rice). However, strain PA13 has shown immense potential in synthetic biology and metabolic engineering, particularly due to its efficient biomass conversion capabilities and robust tolerance to environmental stressors like high osmolarity and temperature fluctuations. PA13 is frequently utilized as a chassis for producing high-value chemicals (such as carotenoids and amino acids) and as a genetic model to study plant-microbe interactions.

BioVector? PA13 技術(shù)說明書 (Technical Datasheet)

中文版說明書 (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? Pantoea ananatis PA13 菌株

• 生物分類： 泛菌屬 (Pantoea)

• 基因組特征： 含有單圓染色體，部分變體攜帶質(zhì)粒；具有完整的類胡蘿卜素合成基因簇。

• 主要特性： 寬底物利用譜（可利用木糖、葡萄糖等）；極強(qiáng)的遺傳操縱抗性穩(wěn)定性。

2. 培養(yǎng)條件

• 培養(yǎng)基： BioVector? LB 培養(yǎng)基或 M9 最小培養(yǎng)基（用于代謝分析）。

• 培養(yǎng)溫度： 最適生長溫度為 30 - 37 攝氏度。

• 培養(yǎng)環(huán)境： 好氧培養(yǎng)，建議搖床轉(zhuǎn)速為 200-220 rpm。

• 篩選抗性： 天然對部分抗生素具有抗性，實驗室改造通常使用卡那霉素或氨芐青霉素。

3. 菌株應(yīng)用

• 類胡蘿卜素生產(chǎn)： 利用其內(nèi)源性類胡蘿卜素途徑，通過代謝調(diào)控生產(chǎn)番茄紅素、玉米黃質(zhì)。

• 生物燃油研究： 作為底盤細(xì)胞轉(zhuǎn)化農(nóng)業(yè)廢棄物（木質(zhì)纖維素）為生物乙醇。

• 植物病理研究： 研究群體感應(yīng)（Quorum Sensing）系統(tǒng)如何調(diào)節(jié)其在寄主植物中的定殖和致病性。

4. 注意事項

• 致病性級別： 雖然 PA13 主要用于科研，但鑒于其對某些植物的潛在致病性，應(yīng)在生物安全一級（BSL-1）或二級（BSL-2，取決于當(dāng)?shù)匾?guī)定）下操作。

• 遺傳穩(wěn)定性： 在大規(guī)模發(fā)酵過程中，建議定期通過 PCR 驗證插入基因片的完整性。

English Datasheet

1. General Product Information

• Product Name: BioVector? Pantoea ananatis PA13 Strain

• Biological Classification: Genus Pantoea

• Genomic Features: Single circular chromosome; some variants carry plasmids; contains a complete endogenous carotenoid biosynthetic gene cluster.

• Key Characteristics: Broad substrate utilization profile (e.g., xylose, glucose); highly amenable to genetic manipulation with stable transgene maintenance.

2. Culture Conditions

• Media: BioVector? LB Medium or M9 Minimal Medium (for metabolic profiling).

• Incubation Temperature: Optimal growth at 30°C - 37°C.

• Culture Environment: Aerobic; recommended shaking speed at 200-220 rpm.

• Selection Markers: Exhibits natural resistance to certain antibiotics; laboratory modifications typically utilize Kanamycin or Ampicillin.

3. Applications

• Carotenoid Production: Leveraging endogenous pathways for the metabolic engineering of lycopene, zeaxanthin, and other pigments.

• Biofuel Research: Functioning as a chassis for converting lignocellulosic biomass (agricultural waste) into bioethanol or organic acids.

• Plant-Microbe Interactions: Investigating how Quorum Sensing systems regulate colonization and pathogenicity in host plant tissues.

4. Key Usage Notes

• Biosafety Level: While PA13 is a research-grade strain, due to its potential phytopathogenicity in specific crops, it should be handled under BSL-1 or BSL-2 conditions depending on regional agricultural regulations.

• Metabolic Flux: PA13 has a unique metabolic network that favors the non-mevalonate (MEP) pathway, which is critical for isoprenoid-derived product yields.

注意 / Note: BioVector? PA13 具有極強(qiáng)的環(huán)境適應(yīng)性。在進(jìn)行基因編輯實驗（如 CRISPR/Cas9）時，建議針對 Pantoea 屬特有的密碼子偏好性進(jìn)行載體優(yōu)化。BioVector? PA13 exhibits exceptional environmental adaptability. For gene editing experiments (e.g., CRISPR/Cas9), BioVector? recommends optimizing vector sequences for the specific codon usage bias of the Pantoea genus.

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