BioVector? PLB-985 人急性髓系白血病細(xì)胞系 / BioVector? PLB-985 Human Acute Myeloid Leukemia Cell Line

通用定義 / General Definition:

BioVector? PLB-985 是一種源自人類急性髓系白血病 (AML) 的骨髓樣細(xì)胞系。盡管最初被認(rèn)為是一個(gè)獨(dú)立的細(xì)胞系，但后來(lái)的遺傳鑒定顯示它與 HL-60 細(xì)胞系高度相似。PLB-985 的核心價(jià)值在于其強(qiáng)大的粒細(xì)胞/單核細(xì)胞分化潛能。通過(guò)二甲基亞砜 (DMSO) 或全反式維甲酸 (ATRA) 的誘導(dǎo)，PLB-985 可以高度模擬中性粒細(xì)胞的生理功能，特別是產(chǎn)生活性氧 (ROS) 的能力。它是研究中性粒細(xì)胞功能、NADPH 氧化酶復(fù)合體機(jī)制以及炎癥反應(yīng)的理想模型，常被用于開發(fā)針對(duì)慢性肉芽腫病 (CGD) 的基因療法。

BioVector? PLB-985 is a myeloid cell line derived from human Acute Myeloid Leukemia (AML). Although initially described as a distinct line, subsequent genetic profiling revealed it to be a subline of HL-60. The primary value of PLB-985 lies in its robust potential for granulocytic and monocytic differentiation. Upon induction with agents like DMSO or All-Trans Retinoic Acid (ATRA), PLB-985 cells effectively mimic the physiological functions of mature neutrophils, particularly the ability to generate Reactive Oxygen Species (ROS). It is an ideal model for studying neutrophil biology, the NADPH oxidase complex, and inflammatory responses, and it is frequently used in the development of gene therapies for Chronic Granulomatous Disease (CGD).

BioVector? PLB-985 技術(shù)說(shuō)明書 (Technical Datasheet)

中文版說(shuō)明書 (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? PLB-985 人髓系白血病細(xì)胞

• 組織來(lái)源： 骨髓 (Bone Marrow)

• 生長(zhǎng)特性： 懸浮生長(zhǎng) (Suspension)

• 細(xì)胞形態(tài)： 原始髓系細(xì)胞樣，呈圓形或卵圓形，分化后體積縮小且核漿比改變。

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? RPMI-1640 培養(yǎng)基。

• 血清添加： 10% BioVector? 優(yōu)質(zhì)胎牛血清 (FBS)。

• 培養(yǎng)環(huán)境： 37 攝氏度，5% $CO_2$。

• 維護(hù)密度： 保持細(xì)胞密度在 $2 \times 10^5$ 至 $1 \times 10^6$ cells/mL 之間。

3. 誘導(dǎo)分化方案

• 中性粒細(xì)胞樣分化： 使用 1.25% DMSO 或 1 $\mu$M ATRA 處理 4-6 天。

• 功能鑒定： 誘導(dǎo)后可通過(guò) NBT 還原實(shí)驗(yàn)或細(xì)胞色素 C 還原法檢測(cè)超氧化物產(chǎn)量。

4. 細(xì)胞應(yīng)用

• NADPH 氧化酶研究： 利用 CRISPR/Cas9 敲除特定亞基（如 gp91phox）來(lái)模擬 CGD 病理。

• 趨化性分析： 研究分化后的 PLB-985 細(xì)胞對(duì)趨化因子（如 fMLP）的定向遷移。

• 吞噬作用： 評(píng)估細(xì)胞對(duì)細(xì)菌或熒光顆粒的吞噬效率。

5. 注意事項(xiàng)

• 分化效率： 細(xì)胞的代數(shù)會(huì)顯著影響分化效率，建議使用復(fù)蘇后 15 代以內(nèi)的細(xì)胞進(jìn)行誘導(dǎo)。

• 結(jié)團(tuán)現(xiàn)象： 細(xì)胞在高密度時(shí)可能出現(xiàn)輕微結(jié)團(tuán)，傳代時(shí)應(yīng)輕輕吹打分散。

English Datasheet

1. General Product Information

• Product Name: BioVector? PLB-985 Human Myeloid Leukemia Cell Line

• Tissue Source: Bone Marrow

• Growth Properties: Suspension

• Morphology: Myeloblast-like; round or oval cells that decrease in size and show nuclear remodeling upon differentiation.

2. Culture Conditions

• Basal Medium: BioVector? RPMI-1640 Medium.

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS).

• Incubation: 37 degrees Celsius, 5% $CO_2$.

• Maintenance: Maintain cell density between $2 \times 10^5$ and $1 \times 10^6$ cells/mL.

3. Differentiation Protocol

• Neutrophilic Differentiation: Treat with 1.25% DMSO or 1 $\mu$M ATRA for 4–6 days.

• Functional Validation: Post-differentiation, superoxide production can be measured via Nitroblue Tetrazolium (NBT) reduction or cytochrome c reduction assays.

4. Applications

• NADPH Oxidase Studies: Using CRISPR/Cas9 to knock out specific subunits (e.g., gp91phox) to model Chronic Granulomatous Disease (CGD).

• Chemotaxis Assays: Investigating the directional migration of differentiated PLB-985 cells toward chemoattractants like fMLP.

• Phagocytosis: Evaluating the efficiency of the cells in engulfing bacteria or fluorescently labeled beads.

5. Key Usage Notes

• Passage Effect: Differentiation efficiency declines with increasing passage number; BioVector? recommends using cells within 15 passages post-thawing for induction experiments.

• Cell Density: Overgrowth can lead to spontaneous differentiation or loss of potency; ensure regular subculturing to maintain the undifferentiated precursor state.

注意 / Note: BioVector? PLB-985 應(yīng)在生物安全二級(jí) (BSL-2) 條件下操作。該細(xì)胞系是研究先天免疫防御機(jī)制的高效替代模型，尤其適用于需要大量中性粒細(xì)胞樣細(xì)胞的實(shí)驗(yàn)。

BioVector? PLB-985 should be handled under Biosafety Level 2 (BSL-2). This cell line serves as a high-efficiency alternative model for studying innate immune defense mechanisms, particularly in experiments requiring large quantities of neutrophil-like cells.

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

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