BioVector? LUHMES 人多巴胺能神經(jīng)元前體細(xì)胞系 / BioVector? LUHMES Human Dopaminergic Neuronal Precursor Cell Line

通用定義 / General Definition:BioVector? LUHMES（Lund Human Mesencephalic）是一種源自人類中腦（Mesencephalon）的多巴胺能神經(jīng)元前體細(xì)胞系。該細(xì)胞系通過 v-myc 基因的四環(huán)素受控表達(dá)實(shí)現(xiàn)永生化。LUHMES 的核心優(yōu)勢在于其高度的可塑性：在添加特定因子（如 GDNF 和 dibutyryl cAMP）并下調(diào) v-myc 表達(dá)后，它們可以迅速、高效地分化為具有成熟電生理活性的多巴胺能神經(jīng)元。它是研究帕金森?。≒D）、神經(jīng)毒理學(xué)、多巴胺代謝及神經(jīng)保護(hù)藥物篩選的理想人源細(xì)胞模型。

BioVector? LUHMES (Lund Human Mesencephalic) is a human dopaminergic neuronal precursor cell line derived from the human mesencephalon. The line was immortalized via the tetracycline-regulated expression of the v-myc oncogene. The primary advantage of LUHMES is its high plasticity: upon treatment with specific factors (such as GDNF and dibutyryl cAMP) and the downregulation of v-myc, these cells differentiate rapidly and efficiently into dopaminergic neurons with mature electrophysiological properties. It is a premier human model for studying Parkinson's Disease (PD), neurotoxicology, dopamine metabolism, and neuroprotective drug screening.

BioVector? LUHMES 技術(shù)說明書 (Technical Datasheet)

中文版說明書 (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? LUHMES 人中腦神經(jīng)前體細(xì)胞

• 組織來源： 人胚胎中腦 (Human Embryonic Mesencephalon)

• 生長特性： 貼壁生長（需包被）

• 分化潛能： 可誘導(dǎo)分化為成熟多巴胺能神經(jīng)元（表達(dá)抗酪氨酸羥化酶 TH）

• 細(xì)胞形態(tài)： 增殖期呈雙極性或小多角形；分化后具有長神經(jīng)突起

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? Advanced DMEM/F12 培養(yǎng)基。

• 關(guān)鍵添加劑（增殖期）： N2 補(bǔ)充劑、重組人堿性成纖維細(xì)胞生長因子 (bFGF) 以及四環(huán)素或強(qiáng)力霉素（用于維持 v-myc 表達(dá)）。

• 包被要求： 培養(yǎng)皿必須使用 多聚鳥氨酸 (Poly-L-ornithine) 和 纖連蛋白 (Fibronectin) 進(jìn)行預(yù)包被。

• 培養(yǎng)環(huán)境： 37 攝氏度，5% $CO_2$

• 分化誘導(dǎo)： 移除 bFGF 并添加四環(huán)素（關(guān)閉 v-myc）、cAMP 和 GDNF，誘導(dǎo) 6-10 天。

3. 細(xì)胞應(yīng)用

• 疾病建模： 利用 MPP+ 或 6-OHDA 誘導(dǎo)損傷，模擬帕金森病神經(jīng)元變性。

• 神經(jīng)毒理學(xué)： 評估環(huán)境毒素或化學(xué)品對人源神經(jīng)元的發(fā)育毒性。

• 多巴胺研究： 探索多巴胺轉(zhuǎn)運(yùn)體 (DAT) 功能及突觸傳遞機(jī)制。

4. 注意事項(xiàng)

• 包被質(zhì)量： LUHMES 細(xì)胞對貼壁要求極高，包被不均勻會(huì)導(dǎo)致細(xì)胞分化失敗或成團(tuán)脫落。

• 血清限制： 建議使用無血清培養(yǎng)體系，以保持其神經(jīng)前體特性并控制分化方向。

English Datasheet

1. General Product Information

• Product Name: BioVector? LUHMES Human Mesencephalic Precursor Cell Line

• Tissue Source: Human Embryonic Mesencephalon

• Growth Properties: Adherent (Requires coating)

• Differentiation Potential: Efficiently differentiates into mature dopaminergic neurons (TH-positive).

• Morphology: Bipolar or small polygonal in proliferative phase; extensive neurite outgrowth post-differentiation.

2. Culture Conditions

• Basal Medium: BioVector? Advanced DMEM/F12 Medium.

• Key Supplements (Proliferation): N2 supplement, recombinant human bFGF, and Tetracycline/Doxycycline (to maintain v-myc expression).

• Surface Coating: Plates must be pre-coated with Poly-L-ornithine and Fibronectin.

• Incubation: 37 degrees Celsius, 5% $CO_2$.

• Differentiation Protocol: Removal of bFGF and addition of Tetracycline (to shut down v-myc), cAMP, and GDNF for 6–10 days.

3. Applications

• Disease Modeling: Simulating Parkinson's disease-associated neurodegeneration using MPP+ or 6-OHDA.

• Neurotoxicology: Assessing the developmental or acute neurotoxicity of environmental toxins on human-derived neurons.

• Dopaminergic Biology: Investigating Dopamine Transporter (DAT) kinetics and synaptogenesis.

4. Key Usage Notes

• Coating Consistency: LUHMES cells are highly sensitive to the substrate; inconsistent coating leads to poor attachment and failed neuronal maturation.

• Serum-Free Requirement: BioVector? recommends strictly serum-free conditions to prevent uncontrolled differentiation and maintain the integrity of the precursor pool.

注意 / Note: BioVector? LUHMES 細(xì)胞系應(yīng)在生物安全二級 (BSL-2) 條件下操作。分化后的神經(jīng)元為終末分化狀態(tài)，不再具有增殖能力，因此實(shí)驗(yàn)設(shè)計(jì)需精確計(jì)算初始接種密度。

BioVector? LUHMES should be handled under Biosafety Level 2 (BSL-2). Differentiated neurons are post-mitotic and cannot be subcultured; precise initial seeding density is critical for successful experimental design.

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

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