BioVector? UOK268 人遺傳性平滑肌瘤病和腎細胞癌細胞系 / BioVector? UOK268 Human Hereditary Leiomyomatosis and Renal Cell Cancer Cell Line

通用定義 / General Definition:BioVector? UOK268 是一種源自人類遺傳性平滑肌瘤病和腎細胞癌 (HLRCC) 的腎腫瘤細胞系。與著名的 UOK262 類似，UOK268 也是研究 延胡索酸水合酶 (Fumarate Hydratase, FH) 缺失性腎癌的關鍵生物模型。該細胞系源自一名 HLRCC 患者的原發(fā)性腎腫瘤，具有 FH 基因的純合缺失或功能喪失性突變。FH 的缺失導致細胞內(nèi)延胡索酸（Fumarate）濃度異常升高，進而誘導蛋白質琥珀酸化（Succination）和代謝重編程。UOK268 為科學家提供了比較不同患者來源、不同轉化背景下 HLRCC 腫瘤生物學差異的寶貴資源。

BioVector? UOK268 is a renal tumor cell line derived from a patient with Hereditary Leiomyomatosis and Renal Cell Cancer (HLRCC). Similar to the well-known UOK262, UOK268 is a critical biological model for studying Fumarate Hydratase (FH)-deficient renal cell carcinoma. This cell line was established from the primary renal tumor of an HLRCC patient and features a homozygous deletion or loss-of-function mutation in the FH gene. The absence of FH leads to abnormally high levels of intracellular fumarate, which induces protein succination and metabolic reprogramming. UOK268 provides researchers with a valuable resource to compare biological variations across different patient-derived HLRCC models.

BioVector? UOK268 技術說明書 (Technical Datasheet)

中文版說明書 (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? UOK268 人 HLRCC 腎細胞癌細胞

• 組織來源： 腎臟 (原發(fā)性腎癌)

• 疾病背景： 遺傳性平滑肌瘤病和腎細胞癌 (HLRCC)

• 生長特性： 貼壁生長

• 細胞形態(tài)： 上皮樣，呈多角形，貼壁牢固

2. 培養(yǎng)條件

• 基礎培養(yǎng)基： BioVector? DMEM 培養(yǎng)基（高糖，含 4.5g/L 葡萄糖）。

• 血清添加： 10% BioVector? 優(yōu)質胎牛血清 (FBS)。

• 關鍵添加劑： 建議添加 1mM 丙酮酸鈉 (Sodium Pyruvate) 以支持其特殊的代謝需求。

• 培養(yǎng)環(huán)境： 37 攝氏度，5% CO2

• 傳代比例： 1:2 至 1:4；建議在細胞匯合度達到 85% 時進行傳代。

3. 細胞應用

• 代謝機制研究： 研究延胡索酸積累如何通過抑制脯氨酰羥化酶 (PHDs) 導致 HIF-1$\alpha$ 的穩(wěn)定性增強。

• 蛋白質組學： 鑒定由高濃度延胡索酸引起的異常蛋白質半胱氨酸琥珀酸化修飾。

• 精準醫(yī)療： 篩選能夠針對性殺死 FH 缺失細胞的合成致死（Synthetic Lethality）藥物。

4. 注意事項

• 營養(yǎng)耗竭： 由于 FH 缺失細胞極度依賴糖酵解，培養(yǎng)基中的葡萄糖消耗極快，建議縮短換液周期。

• 鑒定標準： 實驗開始前應驗證其 FH 蛋白缺失以及 2-琥珀酸半胱氨酸 (2-SC) 的高表達。

English Datasheet

1. General Product Information

• Product Name: BioVector? UOK268 Human HLRCC Renal Cell Cancer Cell Line

• Tissue Source: Kidney (Primary Renal Tumor)

• Disease Context: Hereditary Leiomyomatosis and Renal Cell Cancer (HLRCC)

• Growth Properties: Adherent

• Morphology: Epithelial-like, polygonal, and strongly adherent.

2. Culture Conditions

• Basal Medium: BioVector? DMEM (High Glucose, 4.5g/L glucose).

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS).

• Supplements: 1mM Sodium Pyruvate is recommended to support specialized metabolic requirements.

• Incubation: 37 degrees Celsius, 5% CO2.

• Subculturing: 1:2 to 1:4 ratio; subculture when cells reach 85% confluence.

3. Applications

• Metabolic Mechanisms: Investigating how fumarate accumulation enhances HIF-1$\alpha$ stability by inhibiting prolyl hydroxylases (PHDs).

• Proteomics: Identifying aberrant protein cysteine succination modifications caused by high intracellular fumarate concentrations.

• Precision Medicine: Screening for synthetic lethal drug candidates that selectively target FH-deficient cells.

4. Key Usage Notes

• Nutrient Depletion: FH-deficient cells are highly glycolytic and consume glucose rapidly; BioVector? recommends more frequent medium changes to prevent acidification and nutrient exhaustion.

• Characterization: Before initiating experiments, verify the absence of FH protein and the high expression of 2-succinyl cysteine (2-SC) via immunomarkers.

注意 / Note: BioVector? UOK268 應在生物安全二級 (BSL-2) 條件下操作。該細胞系對于研究 HLRCC 這種侵襲性極強的腎癌具有不可替代的作用，建議在實驗中使用低代數(shù)細胞以保持其代謝特征。BioVector? UOK268 should be handled under Biosafety Level 2 (BSL-2) conditions. This cell line is indispensable for studying the highly aggressive HLRCC; BioVector? recommends using low-passage cells to preserve their unique metabolic profile.

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