BioVector? VM7luc4E2 人乳腺癌雌激素受體報(bào)告基因細(xì)胞系 / BioVector? VM7luc4E2 Human Breast Cancer ER Reporter Cell Line

通用定義 / General Definition:

BioVector? VM7luc4E2（又名 VM7 或經(jīng)過改進(jìn)的 BG1Luc4E2）是一種高度敏感的人源報(bào)告基因細(xì)胞系，專門用于檢測(cè)內(nèi)分泌干擾物 (EDCs) 和雌激素活性。該細(xì)胞系由人乳腺腺癌細(xì)胞衍生而來，穩(wěn)定轉(zhuǎn)染了由雌激素應(yīng)答元件 (ERE) 驅(qū)動(dòng)的螢火蟲熒光素酶 (Firefly Luciferase) 報(bào)告基因質(zhì)粒。由于其內(nèi)源性表達(dá)高水平的人雌激素受體（$\alpha$ 和 $\beta$），VM7luc4E2 被美國(guó)環(huán)保署 (EPA) 和 OECD 認(rèn)可為評(píng)估化學(xué)品雌激素受體激動(dòng)或拮抗活性的標(biāo)準(zhǔn)體外實(shí)驗(yàn)?zāi)Ｐ汀?/p>

BioVector? VM7luc4E2 (also known as VM7 or optimized BG1Luc4E2) is a highly sensitive human-derived reporter cell line specifically designed for the detection of Endocrine Disrupting Chemicals (EDCs) and estrogenic activity. Derived from a human breast adenocarcinoma lineage, these cells are stably transfected with a Firefly Luciferase reporter gene plasmid driven by Estrogen Response Elements (ERE). Expressing high levels of endogenous human estrogen receptors ($\alpha$ and $\beta$), VM7luc4E2 is recognized by the US EPA and OECD as a gold-standard in vitro model for assessing the estrogen receptor agonistic or antagonistic activities of chemicals.

BioVector? VM7luc4E2 技術(shù)說明書 (Technical Datasheet)

中文版說明書 (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? VM7luc4E2 雌激素報(bào)告基因細(xì)胞系

• 母本細(xì)胞： VM7 (原名 BG-1, 人乳腺癌細(xì)胞)

• 生長(zhǎng)特性： 貼壁生長(zhǎng)

• 報(bào)告基因： ERE-驅(qū)動(dòng)的螢火蟲熒光素酶 (Firefly Luciferase)

• 細(xì)胞形態(tài)： 上皮樣，呈多角形

2. 培養(yǎng)條件

• 維持培養(yǎng)基： BioVector? DMEM/F12 (無酚紅) 培養(yǎng)基

• 血清添加： 10% BioVector? 優(yōu)質(zhì)胎牛血清 (FBS)

• 篩選抗生素： 含有適量的 G418 以維持報(bào)告基因的穩(wěn)定性。

• 實(shí)驗(yàn)前處理： 為降低背景活性，實(shí)驗(yàn)前通常需在含有 5% 活性炭脫色血清 (DCC-FBS) 的無酚紅培養(yǎng)基中饑餓處理 24-48 小時(shí)。

• 培養(yǎng)環(huán)境： 37 攝氏度，5% CO2

3. 細(xì)胞應(yīng)用

• 內(nèi)分泌干擾篩選： 定量檢測(cè)農(nóng)藥、化妝品成分或工業(yè)化學(xué)品的類雌激素活性。

• 藥物開發(fā)： 評(píng)估新型選擇性雌激素受體調(diào)節(jié)劑 (SERMs) 的效能。

• 環(huán)境監(jiān)測(cè)： 監(jiān)測(cè)水質(zhì)或土壤提取物中的生物有效性雌激素濃度。

4. 注意事項(xiàng)

• 酚紅敏感性： 酚紅具有微弱的雌激素活性，所有功能性實(shí)驗(yàn)必須使用無酚紅培養(yǎng)基。

• 血清選擇： 必須使用經(jīng)過活性炭處理的血清 (DCC-FBS) 以去除內(nèi)源性類固醇激素，否則會(huì)導(dǎo)致背景信號(hào)過高。

English Datasheet

1. General Product Information

• Product Name: BioVector? VM7luc4E2 Human Breast Cancer ER Reporter Cell Line

• Parental Line: VM7 (formerly BG-1, Human Breast Adenocarcinoma)

• Growth Properties: Adherent

• Reporter Gene: ERE-driven Firefly Luciferase

• Morphology: Epithelial-like, polygonal.

2. Culture Conditions

• Maintenance Medium: BioVector? DMEM/F12 (Phenol Red-Free) Medium

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS)

• Selection Marker: Maintain with G418 to ensure the stability of the luciferase expression cassette.

• Assay Preparation: To minimize background signaling, cells are typically starved in medium containing 5% Dextran-Coated Charcoal-stripped FBS (DCC-FBS) for 24-48 hours prior to chemical exposure.

• Incubation: 37 degrees Celsius, 5% CO2.

3. Applications

• Endocrine Disruptor Screening: Quantifying the estrogenic potential of pesticides, cosmetic ingredients, or industrial chemicals (e.g., BPA).

• Pharmaceutical Research: Evaluating the potency of novel Selective Estrogen Receptor Modulators (SERMs).

• Environmental Monitoring: Detecting bioavailable estrogenic activity in water samples or environmental leachates.

4. Key Usage Notes

• Phenol Red Interference: Phenol red acts as a weak estrogen mimic; therefore, Phenol Red-Free media must be used for all transactivation assays.

• Hormone Depletion: The use of DCC-FBS is critical during the assay phase to remove endogenous hormones that would otherwise cause high baseline luciferase activity.

注意 / Note: BioVector? VM7luc4E2 具有極高的靈敏度，操作過程中應(yīng)嚴(yán)格防止實(shí)驗(yàn)室塑料制品的潛在浸出物（如鄰苯二甲酸酯）干擾實(shí)驗(yàn)。

BioVector? VM7luc4E2 is extremely sensitive; precautions should be taken to prevent interference from potential leachable endocrine disruptors (e.g., phthalates) found in laboratory plasticware.

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

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