BioVector? RPMI-8866 人 B 淋巴母細(xì)胞系 / BioVector? RPMI-8866 Human B Lymphoblastoid Cell Line

通用定義 / General Definition:BioVector? RPMI-8866 是一種源自人類外周血的內(nèi)源性 EB 病毒 (EBV) 轉(zhuǎn)化的 B 淋巴母細(xì)胞系。該細(xì)胞系最初建立于 20 世紀(jì) 60 年代（來自一名患有慢性髓細(xì)胞白血病的患者），但由于其成熟的 B 細(xì)胞特征，它被廣泛用作免疫學(xué)研究的標(biāo)準(zhǔn)模型。RPMI-8866 細(xì)胞穩(wěn)定表達(dá)多種細(xì)胞表面標(biāo)志物，包括 CD23 和 MHC II 類分子，使其成為研究 B 細(xì)胞激活、抗原呈遞、免疫球蛋白合成以及細(xì)胞因子調(diào)控的理想工具。

BioVector? RPMI-8866 is a human B lymphoblastoid cell line derived from peripheral blood and transformed by endogenous Epstein-Barr Virus (EBV). Originally established in the 1960s (from a patient with chronic myelogenous leukemia), it has since become a standard model in immunology due to its mature B-cell characteristics. RPMI-8866 cells constitutively express various surface markers, including CD23 and MHC class II molecules, making them an ideal tool for studying B-cell activation, antigen presentation, immunoglobulin synthesis, and cytokine regulation.

BioVector? RPMI-8866 技術(shù)說明書 (Technical Datasheet)

中文版說明書 (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? RPMI-8866 人 B 淋巴母細(xì)胞

• 組織來源： 外周血 (Peripheral Blood)

• 生長特性： 懸浮生長 (Suspension)

• 細(xì)胞形態(tài)： 淋巴母細(xì)胞樣，通常呈圓形，單個(gè)或成簇懸浮。

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? RPMI-1640 培養(yǎng)基

• 血清添加： 10% - 15% BioVector? 優(yōu)質(zhì)胎牛血清 (FBS)

• 培養(yǎng)環(huán)境： 37 攝氏度，5% CO2

• 傳代與維護(hù)： 細(xì)胞密度應(yīng)維持在 $2 \times 10^5$ 至 $1 \times 10^6$ cells/mL 之間。傳代時(shí)只需通過離心或稀釋方式補(bǔ)充新鮮培養(yǎng)基，無需消化處理。

3. 細(xì)胞應(yīng)用

• 免疫學(xué)研究： 研究 CD23（低親和力 IgE 受體）的表達(dá)及其在過敏反應(yīng)中的作用。

• 細(xì)胞因子分析： 評(píng)估 IL-4、IL-13 等細(xì)胞因子對(duì) B 細(xì)胞功能的調(diào)節(jié)。

• EBV 病毒研究： 作為 EB 病毒永生化 B 細(xì)胞的模型，研究病毒潛伏感染機(jī)制。

4. 注意事項(xiàng)

• 結(jié)團(tuán)現(xiàn)象： RPMI-8866 細(xì)胞在生長良好時(shí)傾向于形成明顯的細(xì)胞團(tuán)簇，這屬于正常生理狀態(tài)，通常通過輕輕吹打即可分散。

• 安全性： 由于該細(xì)胞系含有內(nèi)源性 EB 病毒，必須在生物安全二級(jí) (BSL-2) 環(huán)境下操作。

English Datasheet

1. General Product Information

• Product Name: BioVector? RPMI-8866 Human B Lymphoblastoid Cell Line

• Tissue Source: Peripheral Blood

• Growth Properties: Suspension

• Morphology: Lymphoblast-like; typically round cells growing individually or in clusters.

2. Culture Conditions

• Basal Medium: BioVector? RPMI-1640 Medium

• Serum Supplement: 10% - 15% BioVector? Fetal Bovine Serum (FBS)

• Incubation: 37 degrees Celsius, 5% CO2

• Maintenance: Maintain cell density between $2 \times 10^5$ and $1 \times 10^6$ cells/mL. Subculture by dilution or centrifugation with fresh medium; no trypsinization is required for suspension cells.

3. Applications

• Immunology Research: Studying the expression of CD23 (the low-affinity IgE receptor) and its role in allergic responses.

• Cytokine Signaling: Evaluating the modulation of B-cell functions by cytokines such as IL-4 and IL-13.

• EBV Pathogenesis: Serving as a model for EBV-immortalized B cells to investigate viral latency and oncogenesis.

4. Key Usage Notes

• Cell Clumping: It is characteristic for healthy RPMI-8866 cells to form clusters in suspension. These aggregates can be gently dissociated by pipetting during subculturing.

• Safety Protocols: As the cell line harbors endogenous Epstein-Barr Virus, it must be handled under Biosafety Level 2 (BSL-2) guidelines.

注意 / Note: BioVector? 建議定期檢查培養(yǎng)物的細(xì)胞密度，避免過度匯合導(dǎo)致營養(yǎng)耗竭。為確保實(shí)驗(yàn)的科學(xué)性，建議定期進(jìn)行支原體檢測(cè)。

BioVector? recommends regular monitoring of cell density to prevent nutrient depletion from overgrowth. Periodic mycoplasma screening is highly advised to ensure the integrity of your research.

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