BioVector? MDCK-SIAT1 犬腎上皮細胞系 / BioVector? MDCK-SIAT1 Canine Kidney Epithelial Cell Line

通用定義 / General Definition:

BioVector? MDCK-SIAT1 是一種經(jīng)過穩(wěn)定轉(zhuǎn)染的人源 SIAT1 (ST6Gal-I, $\alpha$2,6-唾液酸轉(zhuǎn)移酶) 基因的 MDCK（Madin-Darby 犬腎細胞） 衍生細胞系。該細胞系的設(shè)計初衷是為了解決普通 MDCK 細胞在流感病毒研究中的局限性。人流感病毒優(yōu)先結(jié)合 $\alpha$2,6-連接的唾液酸受體，而普通 MDCK 細胞表面主要表達 $\alpha$2,3-連接受體。通過過表達 SIAT1，MDCK-SIAT1 細胞能夠模擬人類上呼吸道上皮的受體分布，從而顯著提高人流感病毒的分離率和滴度，是流感病毒監(jiān)測和疫苗研究的行業(yè)標(biāo)準(zhǔn)模型。

BioVector? MDCK-SIAT1 is a derivative of the MDCK (Madin-Darby Canine Kidney) cell line stably transfected with the human SIAT1 (ST6Gal-I, $\alpha$2,6-sialyltransferase) gene. This cell line was engineered to address the limitations of parental MDCK cells in influenza research. Human influenza viruses preferentially bind to $\alpha$2,6-linked sialic acid receptors, whereas standard MDCK cells predominantly express $\alpha$2,3-linked receptors. By overexpressing SIAT1, MDCK-SIAT1 cells mimic the receptor distribution of the human upper respiratory tract, significantly enhancing the isolation rate and titer of human influenza viruses. It is an industry-standard model for influenza surveillance and vaccine research.

BioVector? MDCK-SIAT1 技術(shù)說明書 (Technical Datasheet)

中文版說明書 (Chinese Datasheet)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? MDCK-SIAT1 犬腎上皮細胞系

• 母本細胞： MDCK (NBL-2)

• 物種： 犬 (Canine) / 人 (Human SIAT1 基因)

• 生長特性： 貼壁生長

• 細胞形態(tài)： 上皮樣，呈典型的鋪路石狀排列

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? DMEM 或 MEM 培養(yǎng)基

• 血清添加： 10% BioVector? 優(yōu)質(zhì)胎牛血清 (FBS)

• 篩選抗生素： 含有適量的 G418 (Geneticin)，以維持 SIAT1 基因的穩(wěn)定表達。

• 培養(yǎng)環(huán)境： 37 攝氏度，5% CO2

• 傳代比例： 1:3 至 1:6；建議在細胞匯合度達到 90% 時傳代。

3. 細胞應(yīng)用

• 病毒分離與鑒定： 特別是針對季節(jié)性流感病毒（A型和B型）的高效率分離。

• 抗病毒藥物篩選： 評估神經(jīng)氨酸酶抑制劑等藥物對人流感病毒的抑制效果。

• 受體結(jié)合分析： 研究流感病毒血凝素 (HA) 與 $\alpha$2,6-唾液酸受體的相互作用。

4. 注意事項

• 受體完整性： 病毒感染實驗前，需確保細胞培養(yǎng)基中不含外源神經(jīng)氨酸酶。

• 維持抗性： 長期培養(yǎng)需添加 G418，否則 SIAT1 表達量可能會隨代數(shù)增加而下降，導(dǎo)致對人流感病毒敏感性降低。

English Datasheet

1. General Product Information

• Product Name: BioVector? MDCK-SIAT1 Canine Kidney Epithelial Cell Line

• Parental Line: MDCK (NBL-2)

• Species: Canine (Parent) / Human (SIAT1 transgene)

• Growth Properties: Adherent

• Morphology: Epithelial-like, exhibiting a classic cobblestone appearance.

2. Culture Conditions

• Basal Medium: BioVector? DMEM or MEM Medium

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS)

• Selection Marker: Supplemented with G418 (Geneticin) to ensure stable maintenance of the human SIAT1 expression cassette.

• Incubation: 37 degrees Celsius, 5% CO2

• Subculturing: 1:3 to 1:6 ratio; subculture when cells reach 90% confluence.

3. Applications

• Virus Isolation and Characterization: Highly efficient isolation of seasonal human influenza A and B viruses.

• Antiviral Screening: Evaluating the efficacy of neuraminidase inhibitors and other candidates against humanized viral targets.

• Binding Assays: Studying the interaction between influenza Hemagglutinin (HA) and $\alpha$2,6-linked sialic acid receptors.

4. Key Usage Notes

• Selection Pressure: Continuous cultivation requires G418 to prevent loss of the SIAT1 transgene, which would reduce the cell's sensitivity to human influenza strains.

• Infection Protocols: For influenza infection, cells must be washed to remove serum (which contains inhibitors) and supplemented with TPCK-treated trypsin to facilitate viral entry.

注意 / Note: BioVector? MDCK-SIAT1 應(yīng)在生物安全二級 (BSL-2) 條件下操作。為保證病毒滴度，建議使用低代數(shù)細胞進行感染實驗。

BioVector? MDCK-SIAT1 should be handled under Biosafety Level 2 (BSL-2) conditions. To ensure high viral titers, BioVector? recommends using low-passage cells for all infection studies.

BioVector NTCC質(zhì)粒載體菌株細胞蛋白抗體基因保藏中心

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