BioVector? pYTU植物基因編輯載體 / pYTU Vector

通用定義 / General Definition:BioVector? pYTU 是一種專門(mén)用于植物合成生物學(xué)和基因編輯的二元表達(dá)載體。它是 pY 系列載體（如 pYUU, pYTU）的成員，通?；?pCAMBIA 骨架構(gòu)建。pYTU 的核心設(shè)計(jì)目的是通過(guò) Agrobacterium（農(nóng)桿菌） 介導(dǎo)的轉(zhuǎn)化，在植物中實(shí)現(xiàn)基因的高效表達(dá)或 CRISPR/Cas9 介導(dǎo)的基因組編輯。其名稱中的 "U" 通常代表 Ubiquitin（泛素）啟動(dòng)子，用于驅(qū)動(dòng)核心組件（如 Cas9 或目的基因）在植物組織中的組成型強(qiáng)表達(dá)。

BioVector? pYTU is a binary expression vector specifically designed for plant synthetic biology and genome editing. As a member of the pY vector series (e.g., pYUU, pYTU), it is typically built upon the pCAMBIA backbone. The primary purpose of pYTU is to facilitate efficient gene expression or CRISPR/Cas9-mediated genome editing in plants via Agrobacterium-mediated transformation. The "U" in its designation often signifies the use of a Ubiquitin promoter to drive the constitutive high-level expression of core components, such as Cas9 or the gene of interest (GOI), across various plant tissues.

BioVector? pYTU 技術(shù)說(shuō)明書(shū) (Technical Datasheet)

中文版說(shuō)明書(shū)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? pYTU 植物表達(dá)載體

• 載體類型： 二元載體 (Binary Vector)

• 啟動(dòng)子： Ubiquitin (泛素啟動(dòng)子，通常為 Arabidopsis 或 Maize 來(lái)源)

• 應(yīng)用領(lǐng)域： 植物基因過(guò)表達(dá)、CRISPR/Cas9 基因編輯、合成生物學(xué)元件組裝

• 抗性基因： * 大腸桿菌/農(nóng)桿菌： 卡那霉素 (Kanamycin) 或 大觀霉素 (Spectinomycin)

  • 植物篩選： 潮霉素 (Hygromycin) 或 除草劑 (Basta/PPT)；部分版本支持 YFP 種子熒光篩選

2. 核心元件功能

• LB/RB 邊界： T-DNA 左右邊界，確保外源片段精確整合進(jìn)植物基因組。

  
  

• Cas9/目的基因框： 由強(qiáng) Ubiquitin 啟動(dòng)子驅(qū)動(dòng)，適用于雙子葉和單子葉植物。

• gRNA 表達(dá)框： 通常攜帶 U6 或 U3 啟動(dòng)子，用于驅(qū)動(dòng) CRISPR 引導(dǎo) RNA。

3. 轉(zhuǎn)化條件

• 大腸桿菌宿主： BioVector? DH5-alpha 或 Stbl3。

• 農(nóng)桿菌宿主： BioVector? EHA105, LBA4404 或 GV3101。

English Datasheet

1. General Product Information

• Product Name: BioVector? pYTU Plant Expression Vector

• Vector Type: Binary Vector

• Promoter: Ubiquitin (commonly sourced from Arabidopsis or Maize)

• Applications: Plant gene overexpression, CRISPR/Cas9 genome editing, and synthetic biology circuit assembly.

• Selection Markers: * Bacterial (E. coli/Agro): Kanamycin or Spectinomycin.

  • Plant Selection: Hygromycin or Basta (PPT); certain variants support YFP fluorescence-based seed selection.

2. Core Element Functions

• LB/RB Borders: T-DNA Left and Right Borders, ensuring precise integration of the DNA fragment into the plant genome.

• Expression Cassette: Driven by a robust Ubiquitin promoter, making it suitable for high-level expression in both dicots and monocots.

• gRNA Module: Typically contains a U6 or U3 promoter for driving the expression of CRISPR guide RNAs.

3. Transformation Guidelines

• E. coli Strains: BioVector? DH5-alpha or Stbl3.

• Agrobacterium Strains: BioVector? EHA105, LBA4404, or GV3101.

Note: For CRISPR applications, ensure that the gRNA target sequence is correctly cloned into the pYTU scaffold. BioVector? recommends verifying the final construct via sequencing across the promoter-insert junction. When using YFP-based selection, high-resolution fluorescence microscopy is required for identifying transgenic seeds.

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