BioVector? BL-29 人鼻咽癌細胞系 / BL-29 Human Nasopharyngeal Carcinoma Cell Line

通用定義 / General Definition:BioVector? BL-29 是一種源自人類鼻咽癌 (Nasopharyngeal Carcinoma, NPC) 的永生化上皮細胞系。它是研究鼻咽癌生物學特性的經(jīng)典體外模型之一。BL-29 細胞常被用于探索 NPC 細胞的代謝重編程、細胞信號傳導失調(diào)以及對放射治療和化學治療藥物的反應機制。與 BL-31 和 BL-33 類似，該細胞系在模擬東亞高發(fā)的鼻咽惡性腫瘤方面具有重要的科研價值。

BioVector? BL-29 is an immortalized epithelial cell line derived from Human Nasopharyngeal Carcinoma (NPC). It is one of the classic in vitro models used to study the biological characteristics of nasopharyngeal cancer. BL-29 cells are frequently utilized to explore metabolic reprogramming, dysregulated cellular signaling, and the mechanisms of response to radiotherapy and chemotherapeutic agents. Similar to BL-31 and BL-33, this cell line holds significant research value in modeling nasopharyngeal malignancies, which have a high incidence in East Asia.

BioVector? BL-29 技術(shù)說明書 (Technical Datasheet)

中文版說明書

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? BL-29 人鼻咽癌細胞

• 組織來源： 鼻咽 (Nasopharynx)

• 疾病背景： 鼻咽癌 (NPC)

• 生長特性： 貼壁生長

• 細胞形態(tài)： 上皮樣 (Epithelial-like)，呈多角形，排列緊密

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? RPMI-1640 或 DMEM 培養(yǎng)基

• 血清添加： 10% BioVector? 優(yōu)質(zhì)胎牛血清 (FBS)

• 培養(yǎng)環(huán)境： 37 攝氏度，5% CO2，飽和濕度

• 傳代比例： 1:3 至 1:4；建議在細胞匯合度達到 85% 左右時進行傳代。

3. 細胞應用

• 放療敏感性評估： 研究 BL-29 細胞在電離輻射后的 DNA 損傷修復能力。

• 信號通路分析： 探索調(diào)控 NPC 生長和存活的激酶通路（如 PI3K/Akt, MAPK 等）。

• 非編碼 RNA 研究： 鑒定影響鼻咽癌進展的特異性 miRNA 或 lncRNA。

4. 注意事項

• 細胞系驗證： 由于 NPC 細胞系數(shù)量較多，建議使用 BioVector? 提供的 STR 鑒定數(shù)據(jù)進行對比，防止交叉污染。

• 低氧實驗： NPC 腫瘤常伴有缺氧微環(huán)境，BL-29 細胞在低氧誘導下表現(xiàn)出顯著的表型改變。

English Datasheet

1. General Product Information

• Product Name: BioVector? BL-29 Human Nasopharyngeal Carcinoma Cell Line

• Tissue Source: Nasopharynx

• Disease Context: Nasopharyngeal Carcinoma (NPC)

• Growth Properties: Adherent

• Morphology: Epithelial-like, polygonal, growing in dense clusters.

2. Culture Conditions

• Basal Medium: BioVector? RPMI-1640 or DMEM Medium

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS)

• Incubation: 37 degrees Celsius, 5% CO2, Saturated Humidity

• Subculturing: 1:3 to 1:4 ratio; subculture when cells reach approximately 85% confluence.

3. Applications

• Radiosensitivity Assessment: Investigating the DNA damage repair capacity of BL-29 cells following ionizing radiation.

• Signaling Pathway Analysis: Exploring kinase pathways (e.g., PI3K/Akt, MAPK) that regulate growth and survival in NPC.

• Non-coding RNA Research: Identifying specific miRNAs or lncRNAs that influence the progression of nasopharyngeal cancer.

4. Key Usage Notes

• Cell Authentication: Due to the large number of existing NPC cell lines, BioVector? strongly recommends comparing results with STR profiling data to prevent cross-contamination.

• Hypoxia Studies: Since NPC tumors often harbor a hypoxic microenvironment, BL-29 cells exhibit significant phenotypic changes when cultured under low-oxygen conditions.

Note: BL-29 cells should be handled under Biosafety Level 2 (BSL-2) conditions. To ensure experimental reproducibility, BioVector? recommends using standardized media and high-quality FBS, and suggests maintaining a low passage number during functional assays.

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