BioVector? BL-31 人鼻咽癌細(xì)胞系 / BL-31 Human Nasopharyngeal Carcinoma Cell Line

通用定義 / General Definition:BioVector? BL-31 是一種源自人類鼻咽癌 (Nasopharyngeal Carcinoma, NPC) 的上皮細(xì)胞系。它是鼻咽癌系列研究模型（包括 BL-33, BL-60, BL-89 等）中的一員。該細(xì)胞系保留了鼻咽上皮來源的惡性特征，是研究鼻咽癌細(xì)胞生長調(diào)控、耐藥性演變以及腫瘤發(fā)生過程中遺傳不穩(wěn)定性等基礎(chǔ)問題的寶貴工具。在合成生物學(xué)和腫瘤藥物評(píng)估中，BL-31 常被用作評(píng)估化療藥效和遞送效率的標(biāo)準(zhǔn)化測(cè)試模型。

BioVector? BL-31 is an epithelial cell line derived from Human Nasopharyngeal Carcinoma (NPC). It is a key member of the NPC research model series, which includes lines such as BL-33, BL-60, and BL-89. This cell line retains the malignant characteristics of its nasopharyngeal epithelial origin and serves as a valuable tool for studying fundamental issues such as growth regulation, drug resistance evolution, and genetic instability during oncogenesis. In synthetic biology and oncology drug assessment, BL-31 is frequently used as a standardized model to evaluate the efficacy and delivery efficiency of chemotherapeutics.

BioVector? BL-31 技術(shù)說明書 (Technical Datasheet)

中文版說明書

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? BL-31 人鼻咽癌細(xì)胞

• 組織來源： 鼻咽 (Nasopharynx)

• 疾病背景： 鼻咽癌 (NPC)

• 生長特性： 貼壁生長

• 細(xì)胞形態(tài)： 上皮樣 (Epithelial-like)，呈多角形，貼壁緊密

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? RPMI-1640 或 DMEM 培養(yǎng)基

• 血清添加： 10% BioVector? 優(yōu)質(zhì)胎牛血清 (FBS)

• 培養(yǎng)環(huán)境： 37 攝氏度，5% CO2，恒溫加濕

• 傳代比例： 1:3 至 1:6；建議在細(xì)胞匯合度達(dá)到 80%-90% 時(shí)傳代。

3. 細(xì)胞應(yīng)用

• 藥物敏感性檢測(cè)： 測(cè)試傳統(tǒng)化療藥（如紫杉醇、吉西他濱）或新型靶向藥物對(duì) NPC 的抑制率。

• 凋亡研究： 觀察不同誘導(dǎo)因素（如缺氧、放射、化合物）誘發(fā)的細(xì)胞死亡通路。

• 克隆形成實(shí)驗(yàn)： 評(píng)估單細(xì)胞水平上的增殖潛能和惡性轉(zhuǎn)化程度。

4. 注意事項(xiàng)

• 代數(shù)控制： 為保證實(shí)驗(yàn)數(shù)據(jù)的可重復(fù)性，建議使用復(fù)蘇后 20 代以內(nèi)的細(xì)胞，以防止表型漂變。

• 支原體監(jiān)控： 建議每月進(jìn)行一次支原體污染篩查，BioVector? 推薦配套使用支原體預(yù)防試劑盒。

English Datasheet

1. General Product Information

• Product Name: BioVector? BL-31 Human Nasopharyngeal Carcinoma Cell Line

• Tissue Source: Nasopharynx

• Disease Context: Nasopharyngeal Carcinoma (NPC)

• Growth Properties: Adherent

• Morphology: Epithelial-like, polygonal, and tightly adherent.

2. Culture Conditions

• Basal Medium: BioVector? RPMI-1640 or DMEM Medium

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS)

• Incubation: 37 degrees Celsius, 5% CO2, Humidified Atmosphere

• Subculturing: 1:3 to 1:6 ratio; subculture when cells reach 80%-90% confluence.

3. Applications

• Drug Sensitivity Testing: Measuring the inhibition rates of traditional chemotherapeutics (e.g., Paclitaxel, Gemcitabine) or novel targeted agents on NPC cells.

• Apoptosis Studies: Observing cell death pathways induced by various factors such as hypoxia, radiation, or chemical compounds.

• Colony Formation Assays: Evaluating the proliferative potential and degree of malignant transformation at the single-cell level.

4. Key Usage Notes

• Passage Management: To ensure reproducible results, use cells within 20 passages post-thawing to prevent phenotypic drift.

• Mycoplasma Screening: BioVector? recommends monthly screening for mycoplasma contamination and the use of integrated mycoplasma prevention kits for maintenance.

Note: BL-31 cells should be handled under Biosafety Level 2 (BSL-2) protocols. For consistent signaling and metabolic research, BioVector? advises the use of its pre-screened FBS batches and standardized high-glucose media formulations.

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