BioVector? BL-33 人鼻咽癌細(xì)胞系 / BL-33 Human Nasopharyngeal Carcinoma Cell Line

通用定義 / General Definition:BioVector? BL-33 是一種源自人類鼻咽癌 (Nasopharyngeal Carcinoma, NPC) 的永生化上皮細(xì)胞系。它是鼻咽癌研究領(lǐng)域中常用的系列模型之一（如 BL-33, BL-60, BL-89）。由于鼻咽癌與 EB 病毒 (EBV) 的緊密聯(lián)系，BL-33 常被用于探索病毒基因?qū)λ拗骷?xì)胞信號通路的調(diào)控機(jī)制。該細(xì)胞系具有典型的惡性腫瘤特征，包括高增殖速率、逃避凋亡的能力以及在體外形成細(xì)胞克隆的能力，是篩選針對鼻咽癌靶向治療藥物的重要實(shí)驗(yàn)平臺。

BioVector? BL-33 is an immortalized epithelial cell line derived from Human Nasopharyngeal Carcinoma (NPC). It is part of a common series of NPC models (including BL-33, BL-60, and BL-89) used in oncology research. Given the strong association between NPC and the Epstein-Barr Virus (EBV), BL-33 is frequently employed to explore how viral genes modulate host cell signaling pathways. The cell line exhibits typical malignant characteristics, such as high proliferation rates, evasion of apoptosis, and the ability to form colonies in vitro, making it a vital platform for screening targeted therapeutic agents against nasopharyngeal cancer.

BioVector? BL-33 技術(shù)說明書 (Technical Datasheet)

中文版說明書

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? BL-33 人鼻咽癌細(xì)胞

• 組織來源： 鼻咽 (Nasopharynx)

• 疾病背景： 鼻咽癌 (NPC)

• 生長特性： 貼壁生長

• 細(xì)胞形態(tài)： 上皮樣 (Epithelial-like)，通常呈鋪路石狀貼壁生長

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? RPMI-1640 或 DMEM 培養(yǎng)基

• 血清添加： 10% BioVector? 優(yōu)質(zhì)胎牛血清 (FBS)

• 培養(yǎng)環(huán)境： 37 攝氏度，5% CO2，恒溫加濕

• 傳代比例： 1:3 至 1:6；建議在細(xì)胞匯合度達(dá)到 80%-90% 時進(jìn)行傳代。

3. 細(xì)胞應(yīng)用

• 病毒學(xué)研究： 研究 EB 病毒潛伏膜蛋白 (LMPs) 在 BL-33 細(xì)胞中的表達(dá)及其致癌作用。

• 高通量篩選： 利用 BL-33 細(xì)胞系進(jìn)行小分子干擾 RNA (siRNA) 庫或抗腫瘤藥物庫的篩選。

• 細(xì)胞遷移： 通過劃痕實(shí)驗(yàn)或細(xì)胞侵襲實(shí)驗(yàn)評估 NPC 的轉(zhuǎn)移潛能。

4. 注意事項(xiàng)

• 細(xì)胞鑒定： 為確保實(shí)驗(yàn)的一致性，建議定期進(jìn)行 STR 鑒定，以防止與其他 NPC 細(xì)胞系（如 CNE-1, CNE-2）混淆。

• 血清質(zhì)量： 鼻咽癌細(xì)胞對血清質(zhì)量較為敏感，不合格的血清可能導(dǎo)致細(xì)胞形態(tài)改變或生長減緩，建議使用 BioVector? 驗(yàn)證過的 FBS。

English Datasheet

1. General Product Information

• Product Name: BioVector? BL-33 Human Nasopharyngeal Carcinoma Cell Line

• Tissue Source: Nasopharynx

• Disease Context: Nasopharyngeal Carcinoma (NPC)

• Growth Properties: Adherent

• Morphology: Epithelial-like, typically growing in a cobblestone pattern.

2. Culture Conditions

• Basal Medium: BioVector? RPMI-1640 or DMEM Medium

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS)

• Incubation: 37 degrees Celsius, 5% CO2, Humidified Atmosphere

• Subculturing: 1:3 to 1:6 ratio; subculture when cells reach 80%-90% confluence.

3. Applications

• Virology Research: Investigating the expression and oncogenic roles of Epstein-Barr Virus Latent Membrane Proteins (LMPs) within the BL-33 cells.

• High-Throughput Screening: Utilizing the BL-33 line for siRNA library screens or anti-tumor drug candidate profiling.

• Cell Motility: Assessing the metastatic potential of NPC via wound healing or invasion assays.

4. Key Usage Notes

• Authentication: To maintain experimental integrity, periodic STR profiling is recommended to prevent cross-contamination with other NPC lines like CNE-1 or CNE-2.

• Serum Sensitivity: NPC cell lines can be sensitive to serum quality; poor-grade serum may lead to phenotypic drift or stunted growth. BioVector? recommends using pre-validated, high-quality FBS.

Note: BL-33 cells must be handled under Biosafety Level 2 (BSL-2) protocols. To minimize genetic drift associated with long-term culture, BioVector? advises using low-passage cells for publication-quality experiments.

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

電話:400-800-2947

工作QQ/微信同號:1843439339

網(wǎng)址

http://www.nedfriskphoto.com