BioVector? SL-15 人鼻咽癌細(xì)胞系 / SL-15 Human Nasopharyngeal Carcinoma Cell Line

通用定義 / General Definition:BioVector? SL-15 是一種源自人類鼻咽癌 (Nasopharyngeal Carcinoma, NPC) 的上皮細(xì)胞系。它是研究鼻咽癌生物學(xué)行為的重要體外模型之一。SL-15 細(xì)胞常被用于探索 NPC 的發(fā)病機(jī)制、腫瘤細(xì)胞的遷移與侵襲能力、以及對(duì)新型抗癌藥物（包括化療藥、靶向藥物及天然產(chǎn)物提取物）的敏感性評(píng)價(jià)。在鼻咽癌研究領(lǐng)域，SL-15 與 CNE-1、CNE-2 等細(xì)胞系共同構(gòu)成了研究東亞高發(fā)性鼻咽惡性腫瘤的基礎(chǔ)工具。

BioVector? SL-15 is an epithelial cell line derived from Human Nasopharyngeal Carcinoma (NPC). It serves as a significant in vitro model for investigating the biological characteristics of NPC. SL-15 cells are frequently employed to explore the pathogenesis of NPC, cell migration and invasion capabilities, and the sensitivity to novel anti-cancer agents, including chemotherapeutics, targeted drugs, and natural product extracts. In the field of nasopharyngeal cancer research, SL-15, along with lines like CNE-1 and CNE-2, forms the fundamental toolkit for studying this malignancy, which has a high incidence in East Asia.

BioVector? SL-15 技術(shù)說明書 (Technical Datasheet)

中文版說明書

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? SL-15 人鼻咽癌細(xì)胞

• 組織來源： 鼻咽 (Nasopharynx)

• 疾病背景： 鼻咽癌 (NPC)

• 生長(zhǎng)特性： 貼壁生長(zhǎng)

• 細(xì)胞形態(tài)： 上皮樣 (Epithelial-like)

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? RPMI-1640 或 DMEM 培養(yǎng)基

• 血清添加： 10% BioVector? 優(yōu)質(zhì)胎牛血清 (FBS)

• 培養(yǎng)環(huán)境： 37 攝氏度，5% CO2，飽和濕度

• 傳代比例： 1:3 至 1:4；建議在細(xì)胞匯合度達(dá)到 80% - 90% 時(shí)進(jìn)行傳代。

3. 細(xì)胞應(yīng)用

• 抗腫瘤藥物篩選： 評(píng)估小分子抑制劑對(duì) NPC 細(xì)胞增殖和凋亡的影響。

• 信號(hào)轉(zhuǎn)導(dǎo)研究： 分析調(diào)控鼻咽癌細(xì)胞惡性表型的關(guān)鍵信號(hào)通路（如 Wnt, NF-kB 等）。

• 放射增敏研究： 測(cè)試不同化合物對(duì) SL-15 細(xì)胞放射治療敏感性的增強(qiáng)作用。

4. 注意事項(xiàng)

• STR 鑒定： 鑒于 NPC 細(xì)胞系歷史上存在交叉污染風(fēng)險(xiǎn)，BioVector? 強(qiáng)烈建議定期進(jìn)行 STR 鑒定。

• 無菌操作： 嚴(yán)格遵循無菌技術(shù)，防止支原體及其他微生物污染。

English Datasheet

1. General Product Information

• Product Name: BioVector? SL-15 Human Nasopharyngeal Carcinoma Cell Line

• Tissue Source: Nasopharynx

• Disease Context: Nasopharyngeal Carcinoma (NPC)

• Growth Properties: Adherent

• Morphology: Epithelial-like

2. Culture Conditions

• Basal Medium: BioVector? RPMI-1640 or DMEM Medium

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS)

• Incubation: 37 degrees Celsius, 5% CO2, Saturated Humidity

• Subculturing: 1:3 to 1:4 ratio; subculture when cells reach 80% - 90% confluence.

3. Applications

• Anti-tumor Drug Screening: Assessing the effects of small molecule inhibitors on NPC cell proliferation and apoptosis.

• Signal Transduction: Analyzing key signaling pathways (e.g., Wnt, NF-kB) that regulate the malignant phenotype of nasopharyngeal cancer cells.

• Radiosensitization: Testing the ability of various compounds to enhance the sensitivity of SL-15 cells to radiation therapy.

4. Key Usage Notes

• Cell Authentication: Given the historical risks of cross-contamination among NPC cell lines, BioVector? strongly recommends periodic STR (Short Tandem Repeat) profiling.

• Aseptic Technique: Maintain strict sterile procedures to prevent Mycoplasma and other microbial contaminations.

Note: SL-15 cells should be handled under Biosafety Level 2 (BSL-2) conditions. For optimal experimental results, BioVector? recommends the use of validated culture media and high-grade FBS to ensure consistent cell growth and phenotypic stability.

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