BioVector? Maska-98 人皮膚鱗狀細胞癌細胞系 / Maska-98 Human Cutaneous Squamous Cell Carcinoma Cell Line

通用定義 / General Definition:BioVector? Maska-98 是一種源自人類皮膚鱗狀細胞癌 (Cutaneous Squamous Cell Carcinoma, cSCC) 的細胞系。cSCC 是全球第二常見的皮膚癌，具有潛在的局部侵襲和遠處轉(zhuǎn)移能力。Maska-98 細胞系常被用于研究皮膚腫瘤的發(fā)生、表皮細胞的異常分化、腫瘤微環(huán)境相互作用以及評估針對非黑色素瘤皮膚癌的新型化療藥物、光動力療法 (PDT) 和免疫檢查點抑制劑的藥效。

BioVector? Maska-98 is a cell line derived from Human Cutaneous Squamous Cell Carcinoma (cSCC). cSCC is the second most common form of skin cancer worldwide, characterized by its potential for local invasion and distant metastasis. The Maska-98 cell line is frequently utilized in studies concerning skin oncogenesis, aberrant epidermal differentiation, tumor microenvironment interactions, and the evaluation of novel chemotherapeutics, Photodynamic Therapy (PDT), and immune checkpoint inhibitors specifically for non-melanoma skin cancers.

BioVector? Maska-98 技術說明書 (Technical Datasheet)

中文版說明書

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? Maska-98 人皮膚鱗狀細胞癌細胞

• 組織來源： 皮膚 (Skin)

• 疾病背景： 鱗狀細胞癌 (SCC)

• 生長特性： 貼壁生長

• 細胞形態(tài)： 多角形，典型的上皮樣形態(tài)，常呈成團或島狀生長

2. 培養(yǎng)條件

• 基礎培養(yǎng)基： BioVector? DMEM/F12 或 RPMI-1640 培養(yǎng)基

• 血清添加： 10% BioVector? 優(yōu)質(zhì)胎牛血清 (FBS)

• 關鍵添加劑： 根據(jù)具體研究需要，可能需添加氫化可的松或表皮生長因子 (EGF) 以維持其上皮特性。

• 培養(yǎng)環(huán)境： 37 攝氏度，5% CO2

• 傳代比例： 1:3 至 1:4；建議在細胞匯合度達到 80%-90% 時進行傳代。

3. 細胞應用

• 致癌機制研究： 探索紫外線損傷或特定基因突變（如 TP53, NOTCH）在皮膚癌發(fā)展中的作用。

• 屏障與浸潤： 研究 SCC 細胞如何突破基底膜并向真皮層浸潤。

• 局部治療評估： 測試外用藥物或物理療法對皮膚腫瘤細胞的殺傷效率。

4. 注意事項

• 細胞接觸抑制： 鱗癌細胞在匯合度過高時可能出現(xiàn)分化跡象，影響實驗一致性，建議及時傳代。

• 操作防護： 盡管是貼壁細胞，但在消化過程中應避免劇烈吹打以保持細胞活力。

English Datasheet

1. General Product Information

• Product Name: BioVector? Maska-98 Human Cutaneous Squamous Cell Carcinoma Cell Line

• Tissue Source: Skin

• Disease Context: Squamous Cell Carcinoma (SCC)

• Growth Properties: Adherent

• Morphology: Polygonal, typical epithelial morphology, often growing in clusters or "islands."

2. Culture Conditions

• Basal Medium: BioVector? DMEM/F12 or RPMI-1640 Medium

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS)

• Supplements: Depending on specific research requirements, Hydrocortisone or EGF may be added to maintain the epithelial phenotype.

• Incubation: 37 degrees Celsius, 5% CO2

• Subculturing: 1:3 to 1:4 ratio; subculture when cells reach 80%-90% confluence.

3. Applications

• Oncogenesis Mechanisms: Exploring the roles of UV damage or specific mutations (e.g., TP53, NOTCH) in the progression of skin cancer.

• Invasion Studies: Investigating how SCC cells breach the basement membrane and infiltrate the dermal layers.

• Topical Therapy Assessment: Testing the efficiency of topical pharmacological agents or physical modalities (like PDT) in eradicating skin tumor cells.

4. Key Usage Notes

• Confluence Management: SCC cells may initiate differentiation pathways if allowed to overgrow; maintain timely subculturing to ensure experimental consistency.

• Standard Precautions: Handle under Biosafety Level 2 (BSL-2) conditions. BioVector? recommends the use of validated high-grade FBS to support the robust growth of the Maska-98 line.

Note: For research involving skin barrier modeling, BioVector? Maska-98 can be used in 3D organotypic cultures. To ensure the authenticity of your results, BioVector? advises regular STR profiling and mycoplasma screening.

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