BioVector? MUG-CC1 人透明細(xì)胞軟骨肉瘤細(xì)胞系 / MUG-CC1 Human Clear Cell Chondrosarcoma Cell Line

通用定義 / General Definition:BioVector? MUG-CC1 是一種極其稀有的、源自人類透明細(xì)胞軟骨肉瘤 (Clear Cell Chondrosarcoma, CCC) 的細(xì)胞系。透明細(xì)胞軟骨肉瘤是一種低度惡性的軟骨肉瘤亞型，由于其獨(dú)特的透明細(xì)胞形態(tài)和骨骺端的定位，在臨床上極具特征性。MUG-CC1 由格拉茨醫(yī)學(xué)院（Medical University of Graz）開發(fā)，是目前全球少數(shù)能夠模擬這種罕見骨腫瘤生物學(xué)特性的模型之一。它為研究軟骨基質(zhì)代謝、細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)以及針對(duì)該罕見癌癥的化療敏感性篩選提供了至關(guān)重要的平臺(tái)。

BioVector? MUG-CC1 is an extremely rare cell line derived from Human Clear Cell Chondrosarcoma (CCC). Clear cell chondrosarcoma is a low-grade malignant subtype of chondrosarcoma, clinically characterized by its unique clear cell morphology and epiphyseal localization. Developed by the Medical University of Graz, MUG-CC1 is one of the few models worldwide that recapitulates the biological properties of this rare bone tumor. It provides a crucial platform for studying cartilaginous matrix metabolism, cellular signaling, and chemotherapy sensitivity screening for this rare cancer.

BioVector? MUG-CC1 技術(shù)說明書 (Technical Datasheet)

中文版說明書

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? MUG-CC1 人透明細(xì)胞軟骨肉瘤細(xì)胞

• 組織來源： 骨/軟骨 (Bone/Cartilage)

• 生長(zhǎng)特性： 貼壁生長(zhǎng)

• 細(xì)胞形態(tài)： 上皮樣、多角形，胞質(zhì)清亮，具有典型的透明細(xì)胞特征

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? RPMI-1640 與 DMEM (1:1) 混合培養(yǎng)基

• 血清添加： 10%-15% BioVector? 優(yōu)質(zhì)胎牛血清 (FBS)

• 培養(yǎng)環(huán)境： 37 攝氏度，5% CO2

• 傳代比例： 1:2 至 1:3；由于細(xì)胞倍增時(shí)間較長(zhǎng)，建議在 80%-90% 匯合度時(shí)進(jìn)行傳代

3. 細(xì)胞應(yīng)用

• 罕見腫瘤發(fā)病機(jī)制： 研究導(dǎo)致軟骨肉瘤透明化的分子遺傳學(xué)基礎(chǔ)。

• 生物標(biāo)志物篩選： 鑒定 CCC 特異性的診斷和預(yù)后標(biāo)志物。

• 分化治療研究： 探索誘導(dǎo)惡性軟骨細(xì)胞向成熟軟骨分化的潛在途徑。

4. 注意事項(xiàng)

• 生長(zhǎng)緩慢： 原代來源的罕見腫瘤細(xì)胞系通常生長(zhǎng)較慢，傳代時(shí)需保持較高的接種密度。

• 基質(zhì)依賴： 軟骨細(xì)胞傾向于分泌大量胞外基質(zhì)，培養(yǎng)過程中可能會(huì)觀察到顯著的基質(zhì)沉積。

English Datasheet

1. General Product Information

• Product Name: BioVector? MUG-CC1 Human Clear Cell Chondrosarcoma Cell Line

• Tissue Source: Bone/Cartilage

• Growth Properties: Adherent

• Morphology: Epithelial-like, polygonal with clear cytoplasm, displaying typical clear cell characteristics.

2. Culture Conditions

• Basal Medium: BioVector? RPMI-1640 and DMEM (1:1) mixture

• Serum Supplement: 10%-15% BioVector? Fetal Bovine Serum (FBS)

• Incubation: 37 degrees Celsius, 5% CO2

• Subculturing: 1:2 to 1:3 ratio; due to a relatively long doubling time, subculture when cells reach 80%-90% confluence.

3. Applications

• Rare Tumor Pathogenesis: Investigating the molecular and genetic basis underlying the "clearing" of chondrosarcoma cells.

• Biomarker Screening: Identifying specific diagnostic and prognostic markers for CCC.

• Differentiation Therapy: Exploring potential pathways to induce the differentiation of malignant chondrocytes into mature cartilage.

4. Key Usage Notes

• Slow Proliferation: Rare tumor lines derived from patient tissue often exhibit slower growth rates; ensure a higher seeding density during subculturing to maintain viability.

• Extracellular Matrix: Chondroid cells tend to produce significant amounts of extracellular matrix (ECM), which may lead to visible matrix deposition during culture.

Note: Handle BioVector? MUG-CC1 under Biosafety Level 2 (BSL-2). Regular STR profiling is essential to confirm identity given the rarity of this line. BioVector? recommends the use of its validated specialized media and high-grade FBS to ensure long-term stability of the clear cell phenotype.

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