BioVector? MUG-Lucifer-prim 人骨肉瘤細(xì)胞系 / MUG-Lucifer-prim Human Osteosarcoma Cell Line

通用定義 / General Definition:BioVector? MUG-Lucifer-prim 是一種穩(wěn)定表達(dá)螢火蟲(chóng)熒光素酶 (Firefly Luciferase) 的人源骨肉瘤 (Osteosarcoma) 細(xì)胞系。與高度轉(zhuǎn)移性的 "met" 版本不同，"prim"（源自 Primary）版本代表的是模擬原發(fā)性腫瘤特征的細(xì)胞系。它由奧地利格拉茨醫(yī)學(xué)院建立，通過(guò)整合報(bào)告基因，科研人員可以在非侵入性的情況下，利用生物發(fā)光成像技術(shù) (BLI) 精確監(jiān)測(cè)原位腫瘤的生長(zhǎng)速率、容積變化以及早期定植情況。

BioVector? MUG-Lucifer-prim is a human Osteosarcoma cell line stably expressing the Firefly Luciferase reporter gene. Unlike the highly metastatic "met" variant, the "prim" (derived from Primary) version represents a cell line that mimics the characteristics of the primary tumor. Established by the Medical University of Graz, this line allows researchers to accurately monitor orthotopic tumor growth rates, volume changes, and early colonization in a non-invasive manner using Bioluminescence Imaging (BLI) technology.

BioVector? MUG-Lucifer-prim 技術(shù)說(shuō)明書(shū) (Technical Datasheet)

中文版說(shuō)明書(shū)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱(chēng)： BioVector? MUG-Lucifer-prim 人骨肉瘤細(xì)胞

• 組織來(lái)源： 骨 (Bone)

• 標(biāo)記基因： 螢火蟲(chóng)熒光素酶 (Firefly Luciferase)

• 生長(zhǎng)特性： 貼壁生長(zhǎng)

• 細(xì)胞形態(tài)： 成纖維細(xì)胞樣、多角形

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? RPMI-1640 與 DMEM (1:1) 混合培養(yǎng)基

• 血清添加： 10% BioVector? 優(yōu)質(zhì)胎牛血清 (FBS)

• 篩選/維持抗生素： 根據(jù)載體要求使用博來(lái)霉素 (Zeocin) 或 嘌呤霉素 (Puromycin)，以確保熒光素酶信號(hào)的長(zhǎng)期穩(wěn)定性。

• 培養(yǎng)環(huán)境： 37 攝氏度，5% CO2

• 傳代比例： 1:3 至 1:5；建議在匯合度達(dá)到 80% 時(shí)進(jìn)行傳代。

3. 細(xì)胞應(yīng)用

• 原位腫瘤模型： 構(gòu)建小鼠原位骨肉瘤模型，監(jiān)測(cè)原發(fā)灶的形成。

• 藥效評(píng)估： 用于篩選抑制原發(fā)腫瘤生長(zhǎng)的先導(dǎo)化合物。

• 生物發(fā)光示蹤： 研究原發(fā)腫瘤微環(huán)境與其生長(zhǎng)動(dòng)力學(xué)之間的關(guān)系。

4. 注意事項(xiàng)

• 信號(hào)對(duì)比： 與 "met" 系列相比，"prim" 細(xì)胞通常用于觀察局部腫瘤負(fù)荷。

• 底物處理： 活體實(shí)驗(yàn)前需使用 BioVector? 級(jí) D-熒光素鈉鹽，并確保底物吸收時(shí)間一致以獲得可重復(fù)的數(shù)據(jù)。

English Datasheet

1. General Product Information

• Product Name: BioVector? MUG-Lucifer-prim Human Osteosarcoma Cell Line

• Tissue Source: Bone

• Reporter Gene: Firefly Luciferase

• Growth Properties: Adherent

• Morphology: Fibroblast-like or polygonal

2. Culture Conditions

• Basal Medium: BioVector? RPMI-1640 and DMEM (1:1) mixture

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS)

• Selection/Maintenance: Supplement with appropriate Zeocin or Puromycin to prevent silencing of the Luciferase reporter.

• Incubation: 37 degrees Celsius, 5% CO2

• Subculturing: 1:3 to 1:5 ratio; subculture when cells reach 80% confluence.

3. Applications

• Orthotopic Tumor Models: Establishing localized bone tumor models in mice to monitor primary lesion formation.

• Drug Efficacy Screening: Assessing the effectiveness of lead compounds in suppressing the growth of the primary tumor mass.

• Bioluminescence Tracking: Investigating the correlation between the primary tumor microenvironment and growth kinetics.

4. Key Usage Notes

• Signal Comparison: While "met" cells are used for metastasis studies, the "prim" line is the preferred choice for studying localized tumor burden and primary expansion.

• Substrate Protocol: For in vivo imaging, use BioVector? grade D-Luciferin sodium salt. Ensure consistent substrate incubation times across experimental groups to guarantee reproducible quantification.

Note: To maintain the high luciferase activity of BioVector? MUG-Lucifer-prim, avoid long-term culture without selection pressure. Handle under Biosafety Level 2 (BSL-2) protocols. Periodic STR profiling is recommended for identity verification.

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

電話:400-800-2947

工作QQ/微信同號(hào):1843439339

網(wǎng)址

http://www.nedfriskphoto.com