BioVector? YCUB-2 人腎癌細(xì)胞系 / YCUB-2 Human Renal Cell Carcinoma Cell Line

通用定義 / General Definition:BioVector? YCUB-2 是一種源自人類腎細(xì)胞癌 (Renal Cell Carcinoma, RCC) 的貼壁上皮細(xì)胞系。該細(xì)胞系最初建立于日本（Yokohama City University），主要用于研究腎癌的生物學(xué)行為，尤其是腫瘤的侵襲性、轉(zhuǎn)移潛能以及對不同抗癌藥物的敏感性。由于腎癌通常表現(xiàn)出對傳統(tǒng)放化療的抗性，YCUB-2 提供了一個(gè)關(guān)鍵的體外平臺，用于測試新型免疫療法、激酶抑制劑以及探索腎癌發(fā)生過程中的遺傳改變。

BioVector? YCUB-2 is an adherent epithelial cell line derived from Human Renal Cell Carcinoma (RCC). Originally established in Japan (Yokohama City University), this line is primarily utilized to study the biological behavior of kidney cancer, with a particular focus on tumor invasiveness, metastatic potential, and sensitivity to various anti-cancer agents. Given that renal cell carcinoma often exhibits resistance to traditional radiation and chemotherapy, YCUB-2 provides a critical in vitro platform for testing novel immunotherapies and kinase inhibitors, as well as exploring genetic alterations in renal oncogenesis.

BioVector? YCUB-2 技術(shù)說明書 (Technical Datasheet)

中文版說明書

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? YCUB-2 人腎癌細(xì)胞

• 組織來源： 腎臟 (Kidney)

• 疾病背景： 腎細(xì)胞癌 (RCC)

• 生長特性： 貼壁生長

• 細(xì)胞形態(tài)： 上皮樣 (Epithelial-like)

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? DMEM 或 RPMI-1640 培養(yǎng)基

• 血清添加： 10% BioVector? 優(yōu)質(zhì)胎牛血清 (FBS)

• 培養(yǎng)環(huán)境： 37 攝氏度，5% CO2，飽和濕度

• 傳代比例： 1:3 至 1:6；建議在細(xì)胞匯合度達(dá)到 80%-90% 時(shí)傳代。

3. 細(xì)胞應(yīng)用

• 腫瘤侵襲研究： 利用 Scratch Assay（劃痕實(shí)驗(yàn)）或 Transwell 評價(jià)細(xì)胞的遷移能力。

• 藥物篩選： 評估針對多激酶抑制劑（如索拉非尼、舒尼替尼）的耐藥性及反應(yīng)。

• 生物標(biāo)志物鑒定： 探索腎癌特異性抗原及其在診斷中的潛在應(yīng)用。

4. 注意事項(xiàng)

• 生長密度： 保持細(xì)胞在對數(shù)生長期進(jìn)行實(shí)驗(yàn)，以確保代謝和信號傳導(dǎo)途徑的一致性。

• 安全性： 遵循生物安全二級 (BSL-2) 標(biāo)準(zhǔn)操作。

English Datasheet

1. General Product Information

• Product Name: BioVector? YCUB-2 Human Renal Cell Carcinoma Cell Line

• Tissue Source: Kidney

• Disease Context: Renal Cell Carcinoma (RCC)

• Growth Properties: Adherent

• Morphology: Epithelial-like

2. Culture Conditions

• Basal Medium: BioVector? DMEM or RPMI-1640 Medium

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS)

• Incubation: 37 degrees Celsius, 5% CO2, Saturated Humidity

• Subculturing: 1:3 to 1:6 ratio; subculture when cells reach 80%-90% confluence.

3. Applications

• Tumor Invasion Research: Evaluating cell migration and invasion using scratch assays or Transwell systems.

• Drug Screening: Assessing sensitivity or resistance to multi-kinase inhibitors (e.g., Sorafenib, Sunitinib).

• Biomarker Identification: Exploring RCC-specific antigens and their potential diagnostic applications.

4. Key Usage Notes

• Growth Management: Ensure cells are in the logarithmic growth phase during experiments to maintain consistent metabolic and signaling profiles.

• Safety Protocols: Handle all cultures under Biosafety Level 2 (BSL-2) guidelines as per standard laboratory safety for human tumor cell lines.

Note: For optimal cell viability, BioVector? recommends the use of its validated FBS and high-glucose DMEM. To maintain the purity of the BioVector? YCUB-2 line, periodic STR profiling and mycoplasma screening are strongly advised.

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