BioVector? C17 人鼻咽癌細(xì)胞系 / C17 Human Nasopharyngeal Carcinoma Cell Line

通用定義 / General Definition:BioVector? C17 是一種重要的人類鼻咽癌 (Nasopharyngeal Carcinoma, NPC) 細(xì)胞系。與許多其他在培養(yǎng)過程中丟失病毒基因組的 NPC 細(xì)胞系不同，C17 通常被認(rèn)為是一個(gè)能夠較好保留原始腫瘤生物學(xué)特征的模型。該細(xì)胞系廣泛用于研究鼻咽癌的發(fā)生機(jī)制、上皮-間充質(zhì)轉(zhuǎn)化 (EMT) 過程、腫瘤血管生成以及對(duì)放化療的敏感性。由于鼻咽癌在解剖學(xué)上的特殊性及其與 EB 病毒 (EBV) 的復(fù)雜關(guān)系，C17 提供了研究該特定惡性腫瘤的有力體外平臺(tái)。

BioVector? C17 is a significant Human Nasopharyngeal Carcinoma (NPC) cell line. Unlike many other NPC lines that may lose their viral genome or undergo significant phenotypic shifts during prolonged culture, C17 is recognized as a model that effectively retains many original biological characteristics of the primary tumor. It is widely used to study the pathogenesis of NPC, Epithelial-Mesenchymal Transition (EMT), tumor angiogenesis, and sensitivity to radiotherapy and chemotherapy. Due to the unique anatomical site of NPC and its complex association with Epstein-Barr Virus (EBV), C17 provides a robust in vitro platform for investigating this specific malignancy.

BioVector? C17 技術(shù)說明書 (Technical Datasheet)

中文版說明書

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? C17 人鼻咽癌細(xì)胞

• 組織來源： 鼻咽 (Nasopharynx)

• 生長(zhǎng)特性： 貼壁生長(zhǎng)

• 細(xì)胞形態(tài)： 上皮樣 (Epithelial-like)，呈多角形或鋪路石狀排列

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? RPMI-1640 或 DMEM 培養(yǎng)基

• 血清添加： 10% BioVector? 優(yōu)質(zhì)胎牛血清 (FBS)

• 培養(yǎng)環(huán)境： 37 攝氏度，5% CO2，飽和濕度

• 傳代比例： 1:3 至 1:4；建議在細(xì)胞匯合度達(dá)到 80% 至 90% 時(shí)進(jìn)行傳代。

3. 細(xì)胞應(yīng)用

• 藥物篩選： 評(píng)估新型靶向藥物或免疫調(diào)節(jié)劑對(duì)鼻咽癌細(xì)胞的殺傷效果。

• 轉(zhuǎn)移機(jī)制： 通過 Transwell 實(shí)驗(yàn)研究 NPC 細(xì)胞的遷移和侵襲能力。

• 放射生物學(xué)： 研究 C17 細(xì)胞對(duì) X 射線或伽馬射線照射的反應(yīng)及其修復(fù)機(jī)制。

4. 注意事項(xiàng)

• 細(xì)胞交叉污染： 歷史上部分 NPC 細(xì)胞系曾出現(xiàn)過被 HeLa 細(xì)胞污染的情況，BioVector? 建議對(duì) C17 定期進(jìn)行 STR 鑒定以確保其純度。

• 操作規(guī)范： 盡管部分 NPC 細(xì)胞系不攜帶完整的 EBV，但處理人源腫瘤細(xì)胞時(shí)仍需遵循生物安全二級(jí) (BSL-2) 標(biāo)準(zhǔn)。

English Datasheet

1. General Product Information

• Product Name: BioVector? C17 Human Nasopharyngeal Carcinoma Cell Line

• Tissue Source: Nasopharynx

• Growth Properties: Adherent

• Morphology: Epithelial-like, appearing polygonal or arranged in cobblestone patterns

2. Culture Conditions

• Basal Medium: BioVector? RPMI-1640 or DMEM Medium

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS)

• Incubation: 37 degrees Celsius, 5% CO2, Saturated Humidity

• Subculturing: 1:3 to 1:4 ratio; subculture when cells reach 80% to 90% confluence.

3. Applications

• Drug Screening: Evaluating the cytotoxic effects of novel targeted therapies or immunomodulators on NPC cells.

• Metastasis Mechanisms: Utilizing Transwell assays to investigate the migration and invasive potential of the cells.

• Radiobiology: Studying the response of C17 cells to X-ray or gamma radiation and their subsequent DNA repair mechanisms.

4. Key Usage Notes

• Authentication: Historically, some NPC cell lines have been found to be cross-contaminated with HeLa cells. BioVector? strongly recommends periodic STR (Short Tandem Repeat) profiling to verify the identity and purity of the C17 line.

• Standard Precautions: Even if certain NPC sub-lines do not harbor the full EBV genome, all human tumor cell lines must be handled according to Biosafety Level 2 (BSL-2) guidelines.

Note: For optimal cell recovery and growth, BioVector? recommends using pre-warmed media and avoiding prolonged exposure to trypsin during dissociation. Consistent use of BioVector? validated FBS ensures the stability of the epithelial phenotype.

Supplier生產(chǎn)廠家: BioVector NTCC典型培養(yǎng)物保藏中心

E-mail: [email protected]

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