BioVector? NPC268 人鼻咽癌細(xì)胞系 / NPC268 Human Nasopharyngeal Carcinoma Cell Line

通用定義 / General Definition:BioVector? NPC268 是一種源自人類鼻咽癌 (Nasopharyngeal Carcinoma, NPC) 的永生化上皮細(xì)胞系。鼻咽癌是一種在東亞和東南亞地區(qū)具有高度流行性的頭頸部惡性腫瘤，其發(fā)生與 EB 病毒 (Epstein-Barr Virus, EBV) 感染、環(huán)境因素及遺傳易感性密切相關(guān)。NPC268 細(xì)胞系常被用作體外模型，用于研究鼻咽癌的增殖、侵襲、轉(zhuǎn)移機(jī)制，以及評估化療藥物和靶向治療的有效性。

BioVector? NPC268 is an immortalized epithelial cell line derived from Human Nasopharyngeal Carcinoma (NPC). NPC is a head and neck malignancy with high prevalence in East and Southeast Asia, strongly associated with Epstein-Barr Virus (EBV) infection, environmental factors, and genetic susceptibility. The NPC268 cell line serves as a vital in vitro model for investigating the mechanisms of proliferation, invasion, and metastasis in nasopharyngeal cancer, as well as evaluating the efficacy of chemotherapeutic agents and targeted therapies.

BioVector? NPC268 技術(shù)說明書 (Cell Line Datasheet)

中文版說明書

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? NPC268 人鼻咽癌細(xì)胞

• 組織來源： 鼻咽 (Nasopharynx)

• 疾病背景： 鼻咽癌 (NPC)

• 生長特性： 貼壁生長

• 細(xì)胞形態(tài)： 上皮樣 (Epithelial-like)

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? RPMI-1640 或 DMEM 培養(yǎng)基

• 血清添加： 10% BioVector? 優(yōu)質(zhì)胎牛血清 (FBS)

• 培養(yǎng)環(huán)境： 37 攝氏度，5% CO2，飽和濕度

• 傳代比例： 1:3 至 1:5；建議在細(xì)胞達(dá)到 80% 至 90% 匯合度時進(jìn)行傳代。

3. 細(xì)胞應(yīng)用

• 腫瘤生物學(xué)： 研究調(diào)控 NPC 細(xì)胞生長和凋亡的信號通路。

• 抗藥性研究： 建立耐藥模型以探索鼻咽癌對順鉑等一線化療藥物的耐藥機(jī)制。

• EBV 相關(guān)研究： 探索 EB 病毒基因（如 LMP1）在細(xì)胞轉(zhuǎn)化和維持惡性表型中的作用。

4. 注意事項(xiàng)

• 遺傳穩(wěn)定性： 為防止長期培養(yǎng)導(dǎo)致的遺傳漂變，建議使用低代數(shù)細(xì)胞進(jìn)行實(shí)驗(yàn)并建立種子庫。

• 污染檢測： 定期進(jìn)行支原體檢測，以確保實(shí)驗(yàn)數(shù)據(jù)的準(zhǔn)確性和可靠性。

English Datasheet

1. General Product Information

• Product Name: BioVector? NPC268 Human Nasopharyngeal Carcinoma Cell Line

• Tissue Source: Nasopharynx

• Disease Context: Nasopharyngeal Carcinoma (NPC)

• Growth Properties: Adherent

• Morphology: Epithelial-like

2. Culture Conditions

• Basal Medium: BioVector? RPMI-1640 or DMEM Medium

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS)

• Incubation: 37 degrees Celsius, 5% CO2, Saturated Humidity

• Subculturing: 1:3 to 1:5 ratio; subculture when cells reach 80% to 90% confluence.

3. Applications

• Cancer Biology: Investigating signaling pathways that regulate the growth and apoptosis of NPC cells.

• Drug Resistance: Developing resistance models to explore mechanisms of chemotherapy resistance (e.g., to Cisplatin) in NPC.

• EBV Pathogenesis: Exploring the role of Epstein-Barr Virus proteins (such as LMP1) in cell transformation and the maintenance of malignant phenotypes.

4. Key Usage Notes

• Genetic Integrity: To prevent genetic drift associated with long-term culture, it is highly recommended to use low-passage cells and maintain a localized master cell bank.

• Contamination Control: Routine Mycoplasma testing is essential to ensure the validity and reproducibility of experimental results.

Note: NPC268 cells should be handled under Biosafety Level 2 (BSL-2) conditions. For optimal experimental performance, BioVector? recommends the use of high-grade validated FBS and standardized RPMI-1640 media.

Supplier生產(chǎn)廠家: BioVector NTCC典型培養(yǎng)物保藏中心

E-mail: [email protected]

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