BioVector? HLA B46:01:01:01 人類白細(xì)胞抗原（HLA）B46:01 等位基因表達(dá)質(zhì)粒 / HLA B46:01:01:01 Expression Plasmid

通用定義 / General Definition:BioVector? HLA B46:01:01:01 是一種攜帶人類白細(xì)胞抗原（HLA）B46:01 等位基因全長(zhǎng)編碼序列的表達(dá)質(zhì)粒。HLA-B*46:01 是東亞人群（尤其是漢族）中非常普遍的 I 類 MHC 分子。它在免疫應(yīng)答中起核心作用，負(fù)責(zé)向 CD8+ T 細(xì)胞遞呈內(nèi)源性抗原肽。該等位基因與多種生物醫(yī)學(xué)現(xiàn)象顯著相關(guān)，包括對(duì) SARS-CoV 等病毒的易感性差異、重癥藥物超敏反應(yīng)（如卡馬西平誘導(dǎo)的 SJS/TEN）以及特定的自身免疫性疾病研究。

BioVector? HLA B46:01:01:01 is an expression plasmid containing the full-length coding sequence of the Human Leukocyte Antigen (HLA) B46:01 allele. HLA-B*46:01 is a highly prevalent Class I MHC molecule in East Asian populations, particularly Han Chinese. It plays a pivotal role in the immune response by presenting endogenous antigenic peptides to CD8+ T cells. This specific allele is significantly associated with various biomedical phenomena, including differential susceptibility to viruses like SARS-CoV, severe adverse drug reactions (e.g., Carbamazepine-induced SJS/TEN), and specific autoimmune disease research.

BioVector? HLA B*46:01:01:01 技術(shù)說(shuō)明書(shū) (Plasmid Datasheet)

中文版說(shuō)明書(shū)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? HLA B*46:01:01:01 表達(dá)質(zhì)粒

• 基因簡(jiǎn)稱： HLA-B

• 等位基因全稱： HLA-B*46:01:01:01

• 啟動(dòng)子： CMV 或 EF-1 alpha（取決于具體版本）

• 抗性基因： * 大腸桿菌： 氨芐青霉素 (Ampicillin) 或 卡那霉素 (Kanamycin)

  • 真核篩選： 新霉素 (Neomycin/G418) 或 嘌呤霉素 (Puromycin)

• 標(biāo)簽/融合： 可選 C 端或 N 端攜帶 His/Flag 標(biāo)簽，或無(wú)標(biāo)簽原始序列。

2. 培養(yǎng)與擴(kuò)增條件

• 宿主菌： BioVector? DH5-alpha 或 Stbl3。

• 培養(yǎng)基： BioVector? LB 培養(yǎng)基。

• 環(huán)境： 37 攝氏度，恒溫振蕩。

3. 載體應(yīng)用

• 抗原遞呈研究： 構(gòu)建穩(wěn)定表達(dá) B*46:01 的細(xì)胞系，用于鑒定 T 細(xì)胞表位。

• 藥物基因組學(xué)： 研究特定藥物分子與 HLA-B*46:01 分子結(jié)合導(dǎo)致的免疫毒性。

• 疫苗研發(fā)： 評(píng)估針對(duì)東亞人群流行 HLA 亞型的疫苗免疫原性。

4. 注意事項(xiàng)

• 序列驗(yàn)證： HLA 基因具有高度多態(tài)性，建議使用 BioVector? 專用的 HLA 測(cè)序引物進(jìn)行驗(yàn)證，確保 G-cluster 區(qū)段準(zhǔn)確無(wú)誤。

• 轉(zhuǎn)染效率： 在某些原代淋巴細(xì)胞中轉(zhuǎn)染效率較低，建議結(jié)合 BioVector? 慢病毒包裝系統(tǒng)使用以提高穩(wěn)定性。

English Datasheet

1. General Product Information

• Product Name: BioVector? HLA B*46:01:01:01 Expression Plasmid

• Gene Symbol: HLA-B

• Allele Full Name: HLA-B*46:01:01:01

• Promoter: CMV or EF-1 alpha (variant dependent)

• Selection Markers: * E. coli: Ampicillin or Kanamycin

  • Eukaryotic: Neomycin (G418) or Puromycin

• Tags/Fusion: Available with C-terminal or N-terminal His/Flag tags, or as a tag-free native sequence.

2. Culture and Amplification Conditions

• Host Strains: BioVector? DH5-alpha or Stbl3.

• Culture Medium: BioVector? LB Medium.

• Incubation: 37 degrees Celsius with constant shaking.

3. Applications

• Antigen Presentation Studies: Developing stable B*46:01-expressing cell lines for T-cell epitope mapping.

• Pharmacogenomics: Investigating immune-mediated toxicity caused by the binding of specific drug molecules to the HLA-B*46:01 protein.

• Vaccine Development: Evaluating the immunogenicity of vaccine candidates tailored to prevalent East Asian HLA subtypes.

4. Key Usage Notes

• Sequence Verification: Given the extreme polymorphism of HLA genes, it is critical to use BioVector? specialized HLA sequencing primers to verify the accuracy of the G-cluster and exons.

• Transduction Strategy: Transfection efficiency may be limited in primary lymphocytes; BioVector? recommends subcloning into a lentiviral backbone for stable and high-level expression.

Note: For research involving clinical drug sensitivity, BioVector? provides pre-validated cell lines (e.g., K562 or Jurkat) stably expressing HLA B*46:01:01:01. Always use BioVector? high-purity plasmid prep for functional immunology assays to minimize endotoxin interference.

Supplier生產(chǎn)廠家: BioVector NTCC典型培養(yǎng)物保藏中心

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