BioVector? NHEK 原代正常人表皮角質(zhì)形成細(xì)胞 / Primary Normal Human Epidermal Keratinocytes (NHEK)

通用定義 / General Definition:BioVector? 原代正常人表皮角質(zhì)形成細(xì)胞 (NHEK) 是直接從人類皮膚組織（通常為包皮或手術(shù)切除的皮膚）中分離出來的細(xì)胞。角質(zhì)形成細(xì)胞是表皮的主要構(gòu)成細(xì)胞，占表皮細(xì)胞總數(shù)的 90% 以上。在體外培養(yǎng)中，這些細(xì)胞保持了其在體內(nèi)的生物學(xué)特性，包括增殖能力和向角質(zhì)層分化的潛能。它們是研究皮膚屏障功能、創(chuàng)傷愈合、炎癥反應(yīng)、皮膚毒理學(xué)以及化妝品功效測試的核心細(xì)胞模型。

BioVector? Primary Normal Human Epidermal Keratinocytes (NHEK) are cells isolated directly from human skin tissue (typically neonatal foreskin or adult surgical specimens). Keratinocytes are the predominant cell type in the epidermis, accounting for over 90% of the total epidermal cell population. In vitro, these cells retain their native biological characteristics, including proliferative capacity and the potential to differentiate into the cornified layer. They serve as a fundamental cellular model for studying skin barrier function, wound healing, inflammatory responses, skin toxicology, and cosmetic efficacy testing.

BioVector? NHEK 技術(shù)說明書 (Technical Datasheet)

中文版說明書

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? 原代正常人表皮角質(zhì)形成細(xì)胞

• 細(xì)胞來源： 人類皮膚組織 (Human Skin)

• 生長特性： 貼壁生長

• 細(xì)胞形態(tài)： 鵝卵石樣、典型上皮形態(tài)

• 生物安全性： 所有供體組織均經(jīng)過 HIV, HBV, HCV 等病毒檢測，結(jié)果均為陰性。

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? 角質(zhì)形成細(xì)胞專用無血清培養(yǎng)基 (Keratinocyte Growth Medium, KGM)

• 關(guān)鍵添加劑： BPE (牛垂體提取物)、EGF (表皮生長因子)、胰島素、氫化可的松。

• 培養(yǎng)環(huán)境： 37 攝氏度，5% CO2

• 傳代比例： 1:2 至 1:4；建議在細(xì)胞匯合度達(dá)到 70% 至 80% 時傳代。注意： 原代細(xì)胞不建議培養(yǎng)至 100% 匯合，否則會導(dǎo)致不可逆的分化。

3. 細(xì)胞應(yīng)用

• 皮膚替代模型： 構(gòu)建 3D 有組織表皮模型 (RHE)。

• 屏障功能研究： 檢測細(xì)胞間緊密連接蛋白 (Tight Junctions) 的表達(dá)。

• 臨床前測試： 評估藥物或護(hù)膚品成分的刺激性、過敏性和吸收率。

4. 注意事項

• 鈣離子濃度： 角質(zhì)形成細(xì)胞對鈣離子濃度極度敏感。低鈣培養(yǎng)基維持其增殖，高鈣誘導(dǎo)其分化。

• 有限壽命： 原代細(xì)胞具有有限的倍增次數(shù)（通?？蓚?3 至 5 代），隨著代數(shù)增加，細(xì)胞會逐漸衰老。

English Datasheet

1. General Product Information

• Product Name: BioVector? Primary Normal Human Epidermal Keratinocytes (NHEK)

• Organism: Human Skin Tissue

• Growth Properties: Adherent

• Morphology: Cobblestone-like, typical epithelial morphology

• Biosafety: All donor tissues are screened and negative for HIV-1, HBV, and HCV.

2. Culture Conditions

• Basal Medium: BioVector? Keratinocyte Growth Medium (KGM, Serum-Free)

• Key Supplements: BPE (Bovine Pituitary Extract), EGF, Insulin, and Hydrocortisone.

• Incubation: 37 degrees Celsius, 5% CO2

• Subculturing: 1:2 to 1:4 ratio; subculture at 70% to 80% confluence. Crucial: Never allow primary keratinocytes to reach 100% confluence as this triggers irreversible terminal differentiation.

3. Applications

• Skin Equivalents: Construction of 3D reconstituted human epidermis (RHE) models.

• Barrier Function: Studying the expression of tight junction proteins and lipid metabolism.

• Preclinical Testing: Assessing irritation, sensitization, and absorption of pharmacological or cosmetic compounds.

4. Key Usage Notes

• Calcium Sensitivity: Keratinocyte behavior is strictly regulated by calcium levels. Low calcium concentrations promote proliferation, while higher concentrations (e.g., above 0.1 mM) induce differentiation.

• Finite Lifespan: As primary cells, NHEKs have a limited doubling capacity (typically 3 to 5 passages). Cellular senescence increases with each passage.

Note: To maintain the undifferentiated, proliferative state of BioVector? NHEK cells, it is essential to use BioVector? specialized Serum-Free Medium and minimize exposure to proteolytic enzymes during subculturing. Handle cells with extreme care to ensure high attachment efficiency post-thaw.

Supplier生產(chǎn)廠家: BioVector NTCC典型培養(yǎng)物保藏中心

E-mail: [email protected]

http://www.nedfriskphoto.com