BioVector? pSAT1-mVenus-C (pE3228) 植物熒光表達(dá)載體 / pSAT1-mVenus-C (pE3228) Plant Fluorescence Expression Vector

通用定義 / General Definition:BioVector? pSAT1-mVenus-C (pE3228) 是一種用于植物系統(tǒng)的衛(wèi)星載體 (Satellite Vector)，屬于廣泛使用的 pSAT 系列。該載體設(shè)計(jì)用于在目的蛋白的 C 端 (C-terminus) 融合表達(dá) mVenus（一種單體、增強(qiáng)型黃色熒光蛋白變體）。mVenus 具有極快的成熟速度和更強(qiáng)的抗酸性，使其成為活體植物成像、蛋白質(zhì)定位研究以及雙分子熒光互補(bǔ) (BiFC) 實(shí)驗(yàn)的卓越工具。作為 pSAT 載體，它通常與 pPZP 或 pPZP-RCS 模塊化系統(tǒng)配合使用，通過(guò)罕見(jiàn)限制性內(nèi)切酶（如 AscI）將整個(gè)表達(dá)盒轉(zhuǎn)移到植物二元載體中。

BioVector? pSAT1-mVenus-C (pE3228) is a satellite vector designed for plant systems, belonging to the widely used pSAT series. This vector is engineered to express the mVenus protein (a monomeric, enhanced yellow fluorescent protein variant) fused to the C-terminus of a target protein. mVenus is characterized by its rapid maturation and improved acid stability, making it an excellent tool for live plant imaging, protein localization studies, and Bimolecular Fluorescence Complementation (BiFC) assays. As a pSAT vector, it is typically used in conjunction with the pPZP or pPZP-RCS modular systems, allowing the entire expression cassette to be transferred to a plant binary vector via rare-cutting restriction enzymes like AscI.

BioVector? pSAT1-mVenus-C (pE3228) 技術(shù)說(shuō)明書(shū) (Technical Datasheet)

中文版說(shuō)明書(shū)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? pSAT1-mVenus-C 表達(dá)載體

• 載體編號(hào)： pE3228

• 啟動(dòng)子： 2x35S 啟動(dòng)子（增強(qiáng)型花椰菜花葉病毒 35S 啟動(dòng)子）

• 熒光標(biāo)簽： mVenus (C 端融合)

• 抗性基因： 氨芐青霉素 (Ampicillin)

• 終止子： TL 終止子 (Agrobacterium tumefaciens)

2. 培養(yǎng)與擴(kuò)增條件

• 宿主菌： BioVector? DH5-alpha 或 TOP10。

• 培養(yǎng)基： BioVector? LB 培養(yǎng)基。

• 抗生素： 100 ug/mL 氨芐青霉素。

• 環(huán)境： 37 攝氏度，恒溫振蕩。

3. 載體應(yīng)用

• 亞細(xì)胞定位： 通過(guò)共聚焦顯微鏡觀察目的蛋白在植物細(xì)胞內(nèi)的分布。

• 多色成像： mVenus 的光譜特性允許其與青色 (CFP) 或紅色 (RFP) 熒光蛋白結(jié)合使用。

• BiFC 實(shí)驗(yàn)： 作為 Venus 片段供體，研究植物體內(nèi)的蛋白質(zhì)相互作用。

4. 注意事項(xiàng)

• 移碼風(fēng)險(xiǎn)： 在克隆目的基因時(shí)，請(qǐng)務(wù)必核對(duì)序列，確保目的基因與 C 端的 mVenus 保持正確的讀碼框。

• 模塊化轉(zhuǎn)移： pSAT1 表達(dá)盒側(cè)翼通常帶有罕見(jiàn)酶切位點(diǎn)（如 AscI），便于整體遷移至二元載體進(jìn)行農(nóng)桿菌轉(zhuǎn)化。

English Datasheet

1. General Product Information

• Product Name: BioVector? pSAT1-mVenus-C Expression Vector

• Vector ID: pE3228

• Promoter: Double 35S Promoter (2x35S CaMV)

• Fluorescent Tag: mVenus (C-terminal fusion)

• Antibiotic Resistance: Ampicillin (100 ug/mL)

• Terminator: TL Terminator

2. Culture and Amplification Conditions

• Host Strains: BioVector? DH5-alpha or TOP10.

• Culture Medium: BioVector? LB Medium.

• Incubation: 37 degrees Celsius with constant shaking.

3. Applications

• Subcellular Localization: Visualizing the spatial distribution of target proteins within plant cells via confocal microscopy.

• Multi-color Imaging: The spectral properties of mVenus allow for co-imaging with Cyan (CFP) or Red (RFP) fluorescent proteins.

• Bimolecular Fluorescence Complementation (BiFC): Utilizing Venus fragments for investigating protein-protein interactions in planta.

4. Key Usage Notes

• Frame Alignment: When cloning your Gene of Interest (GOI), ensure there are no internal stop codons and that the GOI is in-frame with the C-terminal mVenus tag.

• Vector Mobilization: The pSAT1 expression cassette is typically flanked by rare restriction sites (e.g., AscI). This allows for easy shuttling into a plant binary vector (such as the pPZP-RCS series) for Agrobacterium-mediated transformation.

Note: To achieve the best imaging results in leaves or protoplasts, BioVector? recommends using high-purity plasmid DNA extracted with BioVector? Endotoxin-Free Kits. For BiFC experiments, verify the orientation and junction sequences using BioVector? sequence validation services.

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