BioVector? FLK-N3 綿羊淋巴母細(xì)胞系 / FLK-N3 Sheep Lymphoblastoid Cell Line

通用定義 / General Definition:BioVector? FLK-N3 是一種源自綿羊（Ovis aries）的淋巴母細(xì)胞系。該細(xì)胞系最顯著的生物學(xué)特性是其作為**牛白血病病毒（Bovine Leukemia Virus, BLV）**的高度敏感宿主和生產(chǎn)細(xì)胞。FLK-N3 細(xì)胞通過持續(xù)性感染 BLV，能夠穩(wěn)定、高效地向培養(yǎng)上清液中釋放病毒顆粒。它是獸醫(yī)學(xué)研究、BLV 病毒學(xué)機制探索以及相關(guān)抗原和疫苗生產(chǎn)的國際標(biāo)準(zhǔn)細(xì)胞模型。

BioVector? FLK-N3 is a lymphoblastoid cell line derived from sheep (Ovis aries). Its most significant biological characteristic is its role as a highly sensitive host and production cell for Bovine Leukemia Virus (BLV). FLK-N3 cells are persistently infected with BLV and stably release high titers of infectious virus particles into the culture supernatant. It serves as an international standard cellular model for veterinary research, investigating BLV virology, and producing antigens or vaccines related to bovine leukosis.

BioVector? FLK-N3 細(xì)胞系技術(shù)說明書 (Cell Line Datasheet)

中文版說明書

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? FLK-N3 綿羊淋巴母細(xì)胞

• 細(xì)胞來源： 綿羊 (Sheep / Ovis aries)

• 組織來源： 胎兒腎臟來源（經(jīng)轉(zhuǎn)化后具有淋巴母細(xì)胞特征）

• 生長特性： 貼壁生長

• 細(xì)胞形態(tài)： 上皮樣或多角形

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? DMEM 培養(yǎng)基 或 Eagle's MEM

• 血清添加： 10% BioVector? 優(yōu)質(zhì)胎牛血清 (FBS)

• 培養(yǎng)環(huán)境： 37 攝氏度，5% CO2，飽和濕度

• 傳代比例： 1:3 至 1:5，當(dāng)細(xì)胞匯合度達到 90% 時進行傳代

3. 細(xì)胞應(yīng)用

• 病毒生產(chǎn)： 大規(guī)模制備牛白血病病毒 (BLV) 顆粒及包膜糖蛋白（如 gp51）。

• 血清學(xué)檢測： 作為 BLV 抗體檢測（如 AGID 或 ELISA）的抗原供體。

• 致癌機制研究： 研究 Deltaretrovirus 屬病毒如何導(dǎo)致宿主細(xì)胞轉(zhuǎn)化和白血病發(fā)生。

4. 注意事項

• 病毒攜帶： 該細(xì)胞系為 BLV 陽性。盡管 BLV 對人類的致病性尚存爭議，但必須在生物安全二級 (BSL-2) 環(huán)境下操作，嚴(yán)防交叉污染。

• 匯合度控制： 維持細(xì)胞處于活躍生長狀態(tài)有助于提高病毒產(chǎn)率，避免長時間過密培養(yǎng)導(dǎo)致細(xì)胞活力下降。

English Datasheet

1. General Product Information

• Product Name: BioVector? FLK-N3 Sheep Lymphoblastoid Cell Line

• Organism: Sheep (Ovis aries)

• Tissue Source: Fetal kidney origin (transformed with lymphoblastoid characteristics)

• Growth Properties: Adherent

• Morphology: Epithelial-like or polygonal

2. Culture Conditions

• Basal Medium: BioVector? DMEM Medium or Eagle's MEM

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS)

• Incubation: 37 degrees Celsius, 5% CO2, Saturated Humidity

• Subculturing: 1:3 to 1:5 ratio; subculture when cells reach 90% confluence

3. Applications

• Virus Production: Large-scale preparation of Bovine Leukemia Virus (BLV) particles and envelope glycoproteins (e.g., gp51).

• Serological Assays: Serving as the antigen source for BLV antibody detection methods like AGID (Agar Gel Immunodiffusion) or ELISA.

• Oncogenesis Research: Investigating how viruses of the Deltaretrovirus genus drive host cell transformation and leukemogenesis.

4. Key Usage Notes

• Viral Status: This cell line is persistently infected with BLV. While the pathogenicity of BLV to humans is a subject of ongoing research, all procedures must be conducted under Biosafety Level 2 (BSL-2) conditions to prevent environmental release or cross-contamination.

• Density Management: Maintaining cells in an active growth phase is critical for maximizing viral yield. Avoid allowing the culture to remain over-confluent for extended periods to preserve cell viability.

Note: For optimal viral replication and protein expression, BioVector? recommends using high-glucose DMEM and validated BioVector? FBS. Regular monitoring for Mycoplasma contamination is essential to ensure experimental reproducibility.

Supplier生產(chǎn)廠家: BioVector NTCC典型培養(yǎng)物保藏中心

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