BioVector? SF8628 人類彌漫性中線膠質(zhì)瘤 (H3.3-K27M) 細(xì)胞系 / SF8628 Human Diffuse Midline Glioma (H3.3-K27M) Cell Line

通用定義 / General Definition:BioVector? SF8628 (H3.3-K27M) 是一種源自兒科彌漫性中線膠質(zhì)瘤（DMG，原稱 DIPG）的腫瘤細(xì)胞系。該細(xì)胞系的核心特征是在 H3F3A 基因中攜帶典型的 K27M 突變。這一突變導(dǎo)致組蛋白 H3.3 的第 27 位賴氨酸被甲硫氨酸取代，進(jìn)而抑制 Polycomb 抑制復(fù)合物 2 (PRC2) 的活性，造成全基因組范圍內(nèi) H3K27me3（三甲基化）水平的大幅下降。它是目前全球范圍內(nèi)研究兒童高惡性膠質(zhì)瘤、表觀遺傳學(xué)調(diào)控及臨床前藥物篩選的關(guān)鍵生物學(xué)模型。

BioVector? SF8628 (H3.3-K27M) is a tumor cell line derived from a pediatric patient with Diffuse Midline Glioma (DMG, formerly known as DIPG). The core characteristic of this cell line is the hallmark K27M mutation in the H3F3A gene. This mutation results in the substitution of lysine 27 with methionine in histone H3.3, which inhibits the activity of the Polycomb Repressive Complex 2 (PRC2) and causes a global reduction in H3K27me3 (trimethylation) levels. It is a pivotal biological model globally for studying pediatric high-grade gliomas, epigenetic regulation, and preclinical drug screening.

BioVector? SF8628 (H3.3-K27M) 技術(shù)說明書 (Technical Datasheet)

中文版說明書

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? SF8628 (H3.3-K27M) 人 DIPG/DMG 細(xì)胞

• 突變信息： H3.3 K27M (H3F3A 突變)

• 生長特性： 貼壁生長（可適應(yīng)懸浮神經(jīng)球培養(yǎng)）

• 細(xì)胞形態(tài)： 成纖維細(xì)胞樣或紡錘形

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? DMEM 培養(yǎng)基 或 DMEM/F12 (1:1)

• 血清添加： 10% BioVector? 優(yōu)質(zhì)胎牛血清 (FBS)

• 培養(yǎng)環(huán)境： 37 攝氏度，5% CO2，飽和濕度

• 傳代比例： 1:3 至 1:6，建議在 80% 到 90% 匯合度時傳代

3. 細(xì)胞應(yīng)用

• 表觀遺傳機制： 研究 H3K27M 突變?nèi)绾瓮ㄟ^重塑染色質(zhì)結(jié)構(gòu)驅(qū)動腫瘤發(fā)生。

• 藥物評價： 篩選針對組蛋白修飾酶（如 HDAC、EZH2）的新型抑制劑。

• 生物標(biāo)志物發(fā)現(xiàn)： 探索 DIPG 進(jìn)展中的新型分子靶點。

4. 注意事項

• 突變驗證： H3.3-K27M 突變是其核心特征，建議定期通過蛋白印跡 (Western Blot) 檢測 K27M 蛋白表達(dá)情況。

• 細(xì)胞干性： 若需研究膠質(zhì)瘤干細(xì)胞特性，建議使用 BioVector? 無血清神經(jīng)干細(xì)胞培養(yǎng)基進(jìn)行誘導(dǎo)。

English Datasheet

1. General Product Information

• Product Name: BioVector? SF8628 (H3.3-K27M) Human DIPG/DMG Cell Line

• Mutation Status: H3.3 K27M (H3F3A mutation)

• Growth Properties: Adherent (adaptable to neurosphere suspension culture)

• Morphology: Fibroblast-like or spindle-shaped

2. Culture Conditions

• Basal Medium: BioVector? DMEM Medium or DMEM/F12 (1:1)

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS)

• Incubation: 37 degrees Celsius, 5% CO2, Saturated Humidity

• Subculturing: 1:3 to 1:6 ratio; recommended at 80% to 90% confluence

3. Applications

• Epigenetic Mechanisms: Investigating how the H3K27M mutation drives tumorigenesis by reshaping chromatin structure.

• Drug Evaluation: Screening novel inhibitors targeting histone-modifying enzymes (e.g., HDAC, EZH2).

• Biomarker Discovery: Exploring new molecular targets in the progression of DIPG.

4. Key Usage Notes

• Mutation Stability: Since H3.3-K27M is the hallmark, periodically verify the K27M protein expression via Western Blotting or DNA sequencing.

• Stemness Maintenance: To study glioma stem cell (GSC) properties, transition the cells into BioVector? serum-free neural stem cell media (supplemented with B27, EGF, and bFGF).

Note: Handle BioVector? SF8628 (H3.3-K27M) cells under Biosafety Level 2 (BSL-2) protocols. To maintain the consistent epigenetic profile of this line, the use of BioVector? validated media and high-grade fetal bovine serum is highly recommended.

Supplier生產(chǎn)廠家: BioVector NTCC典型培養(yǎng)物保藏中心

E-mail: [email protected]

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