BioVector? SF8628 人類彌漫性內(nèi)源性橋腦膠質(zhì)瘤細胞系 / SF8628 Human Diffuse Intrinsic Pontine Glioma (DIPG) Cell Line

通用定義 / General Definition:

BioVector? SF8628 是一種源自兒科彌漫性內(nèi)源性橋腦膠質(zhì)瘤（DIPG）患者的經(jīng)典腫瘤細胞系。該細胞系的關鍵生物學特征是攜帶 $H3K27M$ 突變（組氨酸 3 突變?yōu)榧琢虬彼幔@一突變導致全球性的組蛋白 H3K27 三甲基化（H3K27me3）水平降低，從而重塑表觀遺傳圖譜。SF8628 是目前國際上研究兒科高惡性膠質(zhì)瘤發(fā)病機制、組蛋白突變影響以及篩選表觀遺傳藥物（如 HDAC 抑制劑或 KDM6 抑制劑）的核心模型。

BioVector? SF8628 is a classic tumor cell line derived from a pediatric patient with Diffuse Intrinsic Pontine Glioma (DIPG). The defining biological feature of this cell line is the $H3K27M$ mutation (substitution of lysine 27 with methionine in histone H3), which results in a global reduction of histone H3K27 trimethylation (H3K27me3) levels and a rewired epigenetic landscape. SF8628 is a cornerstone model globally for investigating the pathogenesis of pediatric high-grade gliomas, the functional impact of histone mutations, and screening epigenetic therapeutics such as HDAC or KDM6 inhibitors.

BioVector? SF8628 細胞系技術說明書 (Cell Line Datasheet)

中文版說明書

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? SF8628 人 DIPG 腫瘤細胞

• 細胞來源： 人類 (Human / Pediatric)

• 組織來源： 腦/橋腦 (Brain / Pons)

• 生長特性： 貼壁生長（在特定條件下也可進行神經(jīng)球懸浮培養(yǎng)）

• 細胞形態(tài)： 成纖維細胞樣或多角形

2. 培養(yǎng)條件

• 基礎培養(yǎng)基： BioVector? DMEM 培養(yǎng)基 或 DMEM/F12 (1:1)

• 血清添加： 10% BioVector? 優(yōu)質(zhì)胎牛血清 (FBS)

• 培養(yǎng)環(huán)境： 37°C，5% $CO_2$，飽和濕度

• 可選補充： 部分研究人員使用無血清神經(jīng)干細胞培養(yǎng)基（含 B27, EGF, bFGF）以維持更原始的干性狀態(tài)。

3. 細胞應用

• 表觀遺傳學研究： 研究 $H3K27M$ 突變?nèi)绾螌е禄虮磉_失調(diào)。

• 藥物篩選： 評估針對 DIPG 的新型靶向藥物，特別是表觀遺傳調(diào)節(jié)劑。

• 腫瘤侵襲模型： 用于體外遷移實驗或建立原位異種移植動物模型。

4. 注意事項

• 突變驗證： 由于 $H3K27M$ 是該細胞的核心特征，建議定期通過 Western Blot 檢測 H3K27M 表達及 H3K27me3 的缺失情況。

• 細胞傳代： 建議在細胞達到 80%-90% 匯合時進行傳代。避免細胞密度過高導致生長停滯。

English Datasheet

1. General Product Information

• Product Name: BioVector? SF8628 Human DIPG Tumor Cell Line

• Organism: Human (Pediatric)

• Tissue Source: Brain / Pons

• Growth Properties: Adherent (can be adapted to neurosphere suspension culture in specialized media)

• Morphology: Fibroblast-like or polygonal

2. Culture Conditions

• Basal Medium: BioVector? DMEM Medium or DMEM/F12 (1:1)

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS)

• Incubation: 37°C, 5% $CO_2$, Saturated Humidity

• Alternative Media: Some protocols utilize serum-free neural stem cell media (supplemented with B27, EGF, and bFGF) to maintain stemness features.

3. Applications

• Epigenetics Research: Investigating how the $H3K27M$ mutation drives transcriptional dysregulation.

• Drug Screening: Evaluating novel targeted therapies for DIPG, particularly epigenetic modulators.

• Tumor Invasiveness: Used in in vitro migration assays or for establishing orthotopic xenograft animal models.

4. Key Usage Notes

• Mutation Profiling: As $H3K27M$ is the hallmark of this line, it is critical to periodically verify H3K27M protein expression and the characteristic loss of H3K27me3 via Western Blot.

• Subculturing: Maintain healthy cultures by subculturing at 80%-90% confluence. Avoid overconfluence to prevent shifts in growth kinetics.

Note: BioVector? SF8628 cells are classified under Biosafety Level 2 (BSL-2). To ensure the stability of the epigenetic profile and mutation status, the consistent use of BioVector? DMEM Medium and high-grade BioVector? FBS is strongly recommended.

Supplier生產(chǎn)廠家: BioVector NTCC典型培養(yǎng)物保藏中心

E-mail: [email protected]

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