BioVector? 普通擬桿菌 / Bacteroides vulgatus

通用定義 / General Definition:

BioVector? 普通擬桿菌 (Bacteroides vulgatus) 是一種革蘭氏陰性、專性厭氧、不產(chǎn)生芽孢的桿狀細菌。它是人類腸道菌群（核心菌群）中最豐富的成員之一。該菌在調(diào)節(jié)宿主免疫系統(tǒng)、維持腸道穩(wěn)態(tài)、分解復(fù)雜多糖以及膽汁酸代謝中發(fā)揮著關(guān)鍵作用。在研究領(lǐng)域，它被廣泛用于克羅恩病（CD）、潰瘍性結(jié)腸炎（UC）等炎癥性腸?。↖BD）的發(fā)病機制研究，以及腸道微生物組對肥胖和代謝綜合征的影響研究。

BioVector? Bacteroides vulgatus is a Gram-negative, obligately anaerobic, non-spore-forming, rod-shaped bacterium. It is one of the most abundant members of the human gut microbiome (core microbiota). This bacterium plays a critical role in modulating the host immune system, maintaining intestinal homeostasis, fermenting complex polysaccharides, and metabolizing bile acids. In research, it is widely utilized for studying the pathogenesis of Inflammatory Bowel Disease (IBD), such as Crohn's disease and ulcerative colitis, as well as the impact of the gut microbiome on obesity and metabolic syndrome.

BioVector? Bacteroides vulgatus 技術(shù)說明書 (Technical Datasheet)

中文版說明書

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? 普通擬桿菌

• 菌種學(xué)名：Bacteroides vulgatus

• 分類學(xué)： 擬桿菌門 (Bacteroidetes)，擬桿菌綱 (Bacteroidia)，擬桿菌目 (Bacteroidales)

• 生長特性： 專性厭氧

• 生物安全性： BSL-1/BSL-2（取決于特定菌株和應(yīng)用）

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? 強化梭菌培養(yǎng)基 (RCM) 或 BioVector? BHI 培養(yǎng)基（建議添加血紅素和維生素 K1）

• 血清/添加劑： 5% - 10% BioVector? 脫纖維羊血（可選，用于增強生長）

• 培養(yǎng)環(huán)境： 37°C，絕對厭氧環(huán)境（如 80% $N_2$, 10% $CO_2$, 10% $H_2$）

3. 菌種應(yīng)用

• 腸道穩(wěn)態(tài)研究： 探索擬桿菌對腸道屏障功能的維護作用。

• 免疫免疫調(diào)節(jié)： 研究其與 T 細胞（特別是 Treg 和 Th17）的相互作用。

• 代謝組學(xué)研究： 纖維降解過程中的短鏈脂肪酸 (SCFA) 產(chǎn)量測定。

• IBD 模型： 作為條件致病菌或共生保護菌應(yīng)用于小鼠結(jié)腸炎模型。

4. 注意事項

• 厭氧操作：Bacteroides vulgatus 對氧氣極度敏感。從復(fù)蘇到接種的所有操作應(yīng)在厭氧操作箱中進行。

• 菌種鑒定： 建議使用 BioVector? 16S rRNA 測序或 MALDI-TOF MS 進行定期的菌株純度驗證。

English Datasheet

1. General Product Information

• Product Name: BioVector? Bacteroides vulgatus

• Species:Bacteroides vulgatus

• Taxonomy: Phylum Bacteroidetes, Class Bacteroidia, Order Bacteroidales

• Growth Properties: Obligate Anaerobe

• Biosafety Level: BSL-1 / BSL-2 (depending on strain and local regulations)

2. Culture Conditions

• Basal Medium: BioVector? Reinforced Clostridial Medium (RCM) or BioVector? BHI Medium (supplemented with Hemin and Vitamin K1)

• Supplements: 5% - 10% BioVector? Defibrinated Sheep Blood (optional for enhanced growth)

• Incubation: 37°C, Strict Anaerobic Conditions (e.g., 80% $N_2$, 10% $CO_2$, 10% $H_2$)

3. Applications

• Intestinal Homeostasis: Investigating the role of Bacteroides in maintaining gut barrier function.

• Immunomodulation: Studying interactions with T-cell populations (specifically Treg and Th17).

• Metabolomics: Measuring Short-Chain Fatty Acid (SCFA) production during fiber fermentation.

• IBD Models: Application in murine colitis models as either a pathobiont or a commensal protective agent.

4. Key Usage Notes

• Anaerobic Handling:Bacteroides vulgatus is highly sensitive to oxygen. All procedures from resuscitation to inoculation must be performed within an anaerobic chamber.

• Purity Validation: Periodic verification using BioVector? 16S rRNA sequencing or MALDI-TOF MS is recommended to ensure strain integrity.

Note: For optimal growth and metabolic activity, always use freshly prepared BioVector? Anaerobic Media. Proper use of BioVector? Hemin and Vitamin K1 supplements is critical for the robust expansion of Bacteroides vulgatus cultures.

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