BioVector? mHypoE-38 小鼠下丘腦永生化神經(jīng)元細(xì)胞系 / mHypoE-38 Mouse Immortalized Hypothalamic Neuronal Cell Line

通用定義 / General Definition:

BioVector? mHypoE-38 是一種源自胚胎期（E15、E17、E18）小鼠下丘腦組織的永生化細(xì)胞系。該細(xì)胞系通過逆轉(zhuǎn)錄病毒介導(dǎo)的 SV40 大 T 抗原轉(zhuǎn)導(dǎo)技術(shù)建立。mHypoE-38 屬于“胚胎源性下丘腦細(xì)胞系（Cellutions）”系列，保留了下丘腦神經(jīng)元的許多生理特征。它在體外表達(dá)特定的神經(jīng)肽和受體，是研究中樞神經(jīng)系統(tǒng)對能量代謝、內(nèi)分泌調(diào)控及神經(jīng)分泌信號轉(zhuǎn)導(dǎo)的理想細(xì)胞模型。

BioVector? mHypoE-38 is an immortalized neuronal cell line derived from embryonic mouse hypothalamic tissue (E15, E17, or E18). This line was established using retroviral-mediated transfer of the SV40 large T-antigen. Part of the "Embryonic Hypothalamic Cell Line" series, BioVector? mHypoE-38 retains many physiological characteristics of hypothalamic neurons. It expresses specific neuropeptides and receptors in vitro, serving as an ideal cellular model for studying the central nervous system's role in energy metabolism, endocrine regulation, and neurosecretory signaling pathways.

BioVector? mHypoE-38 細(xì)胞系技術(shù)說明書 (Cell Line Datasheet)

中文版說明書

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? mHypoE-38 小鼠下丘腦永生化神經(jīng)元細(xì)胞

• 細(xì)胞來源： 小鼠 (C57BL/6)

• 組織來源： 下丘腦 (Hypothalamus)

• 生長特性： 貼壁生長

• 細(xì)胞形態(tài)： 神經(jīng)元樣，具有突起結(jié)構(gòu)

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? DMEM 培養(yǎng)基 (含 4.5 g/L 葡萄糖)

• 血清添加： 10% BioVector? 胎牛血清 (FBS)

• 培養(yǎng)環(huán)境： 37°C，5% $CO_2$，飽和濕度

3. 細(xì)胞應(yīng)用

• 神經(jīng)內(nèi)分泌研究： 研究瘦素 (Leptin)、胰島素或其他代謝激素對下丘腦神經(jīng)元的信號傳導(dǎo)。

• 晝夜節(jié)律研究： 探索下丘腦時鐘基因的表達(dá)調(diào)控。

• 神經(jīng)肽分泌： 檢測特定神經(jīng)肽 (如 NPY, AgRP 等) 的表達(dá)與調(diào)節(jié)。

4. 注意事項

• 細(xì)胞匯合度： 建議在細(xì)胞達(dá)到 80%-90% 匯合度時進(jìn)行傳代，避免過度生長導(dǎo)致神經(jīng)突起交織過多而難以消化。

• 特性驗證： 隨代數(shù)增加，建議定期檢測特定神經(jīng)標(biāo)記物或激素受體的表達(dá)，以確保其生理表型穩(wěn)定。

English Datasheet

1. General Product Information

• Product Name: BioVector? mHypoE-38 Mouse Immortalized Hypothalamic Neuronal Cell Line

• Organism: Mouse (C57BL/6)

• Tissue Source: Hypothalamus

• Growth Properties: Adherent

• Morphology: Neuronal, exhibiting neurite extensions

2. Culture Conditions

• Basal Medium: BioVector? DMEM Medium (4.5 g/L Glucose)

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS)

• Incubation: 37°C, 5% $CO_2$, Saturated Humidity

3. Applications

• Neuroendocrine Research: Studying signaling pathways of Leptin, Insulin, or other metabolic hormones in hypothalamic neurons.

• Circadian Rhythm Studies: Investigating the regulation and expression of clock genes in the hypothalamus.

• Neuropeptide Secretion: Monitoring the expression and modulation of specific neuropeptides (e.g., NPY, AgRP).

4. Key Usage Notes

• Confluence Management: Subculture is recommended at 80%-90% confluence to prevent excessive neurite entanglement, which can make enzymatic dissociation difficult.

• Phenotypic Validation: It is advised to periodically verify the expression of specific neuronal markers or hormone receptors across passages to ensure the stability of the BioVector? mHypoE-38 physiological profile.

Note: Handle BioVector? mHypoE-38 cells under Biosafety Level 2 (BSL-2) protocols. For high-fidelity research in neuronal metabolism, the consistent use of BioVector? DMEM Medium and BioVector? Fetal Bovine Serum is essential for maintaining robust cellular responses.

Supplier生產(chǎn)廠家: BioVector NTCC典型培養(yǎng)物保藏中心

E-mail: [email protected]

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