BioVector? Caco-2 BBE 人類結(jié)腸腺癌細(xì)胞系 / Caco-2BBE Human Colorectal Adenocarcinoma Cell Line

通用定義 / General Definition:

BioVector? Caco-2BBE (C2BBe1) 是從 Caco-2 細(xì)胞系中通過(guò)極限稀釋法篩選出的一個(gè)亞克隆。相比于原始的 Caco-2，BioVector? Caco-2BBE 在成層、極化以及刷狀緣（Brush Border）蛋白（如蔗糖酶-異構(gòu)麥芽糖酶）的表達(dá)上表現(xiàn)出更高的一致性和形態(tài)學(xué)上的優(yōu)越性。它能夠形成極其緊密的單層細(xì)胞膜，是目前模擬人小腸上皮屏障、研究微絨毛結(jié)構(gòu)、營(yíng)養(yǎng)吸收以及藥物滲透性（如 Caco-2 滲透實(shí)驗(yàn)）的國(guó)際公認(rèn)標(biāo)準(zhǔn)模型。

BioVector? Caco-2BBE (C2BBe1) is a subclone isolated from the Caco-2 cell line via limiting dilution. Compared to parental Caco-2, BioVector? Caco-2BBE exhibits superior homogeneity in stratification, polarization, and the expression of brush border enzymes (e.g., sucrase-isomaltase). It forms highly organized, confluent monolayers that more closely resemble the human intestinal epithelium. It is the gold-standard model for studying microvilli structure, nutrient absorption, and drug permeability (e.g., Caco-2 permeability assays).

BioVector? Caco-2BBE 細(xì)胞系技術(shù)說(shuō)明書 (Cell Line Datasheet)

中文版說(shuō)明書

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? Caco-2BBE 人結(jié)腸癌細(xì)胞（刷狀緣表達(dá)克隆）

• 細(xì)胞來(lái)源： 人類 (Human)

• 組織來(lái)源： 結(jié)腸/直腸 (Colon/Rectum)

• 生長(zhǎng)特性： 貼壁生長(zhǎng)

• 細(xì)胞形態(tài)： 上皮細(xì)胞樣

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? DMEM 培養(yǎng)基（含 4.5 g/L 葡萄糖）

• 血清添加： 10% BioVector? 胎牛血清 (FBS)

• 添加劑： 0.01 mg/mL BioVector? 人轉(zhuǎn)鐵蛋白 (Human Transferrin)

• 培養(yǎng)環(huán)境： 37°C，5% $CO_2$，飽和濕度

3. 細(xì)胞應(yīng)用

• 藥物滲透性實(shí)驗(yàn)： 測(cè)量藥物穿過(guò)腸道單層的轉(zhuǎn)運(yùn)效率（Papp 值）。

• 細(xì)胞極性研究： 研究頂端 (Apical) 與基底側(cè) (Basolateral) 的蛋白定位。

• 腸道致病菌感染模型： 研究細(xì)菌對(duì)腸道屏障的黏附與侵襲。

4. 注意事項(xiàng)

• 分化周期： 細(xì)胞在達(dá)到 100% 匯合后需要額外的 14-21 天才能完全極化和分化。

• 匯合度管理： 傳代時(shí)應(yīng)避免細(xì)胞長(zhǎng)期處于匯合狀態(tài)，否則會(huì)引起過(guò)早分化，導(dǎo)致實(shí)驗(yàn)結(jié)果不穩(wěn)定。建議在 80% 匯合時(shí)傳代。

English Datasheet

1. General Product Information

• Product Name: BioVector? Caco-2BBE Human Colorectal Adenocarcinoma Cell Line

• Organism: Human

• Tissue Source: Colon / Rectum

• Growth Properties: Adherent

• Morphology: Epithelial-like

2. Culture Conditions

• Basal Medium: BioVector? DMEM Medium (4.5 g/L Glucose)

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS)

• Supplement: 0.01 mg/mL BioVector? Human Transferrin

• Incubation: 37°C, 5% $CO_2$, Saturated Humidity

3. Applications

• Drug Permeability Assays: Measuring the transport efficiency (Papp values) of compounds across the intestinal monolayer.

• Cell Polarity Studies: Investigating protein localization between apical and basolateral membranes.

• Enteric Infection Models: Studying the adhesion and invasion of intestinal pathogens.

4. Key Usage Notes

• Differentiation Period: Cells require an additional 14-21 days post-confluence to achieve full polarization and brush border enzyme maturation.

• Confluence Management: To maintain the proliferative capacity of the stock, do not allow cells to remain confluent for extended periods before subculturing. Subculture is recommended at 80% confluence.

Note: Handle BioVector? Caco-2BBE cells under Biosafety Level 2 (BSL-2) conditions. For consistent results in permeability assays, the use of BioVector? DMEM Medium and BioVector? Fetal Bovine Serum is critical to maintain tight junction integrity (TER values).

Supplier生產(chǎn)廠家: BioVector NTCC典型培養(yǎng)物保藏中心

E-mail: [email protected]

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