BioVector? HASTR/ci35 人類星形膠質(zhì)細(xì)胞永生化細(xì)胞系 / HASTR/ci35 Human Immortalized Astrocyte Cell Line

通用定義 / General Definition:

BioVector? HASTR/ci35 是一種源自人類胎兒腦組織的星形膠質(zhì)細(xì)胞永生化細(xì)胞系。該細(xì)胞系通過轉(zhuǎn)導(dǎo)**溫度敏感型 SV40 大 T 抗原（tsA58）和人端粒酶逆轉(zhuǎn)錄酶（hTERT）**基因建立。其獨(dú)特之處在于其“條件永生化”特性：在 33°C 下持續(xù)增殖，而在 37°-39°C 下則停止分裂并表現(xiàn)出更成熟的星形膠質(zhì)細(xì)胞分化表型。它是研究神經(jīng)炎癥、血腦屏障（BBB）動(dòng)力學(xué)及神經(jīng)退行性疾病中星形膠質(zhì)細(xì)胞功能的卓越工具。

BioVector? HASTR/ci35 is an immortalized human astrocyte cell line derived from fetal brain tissue. The line was established via transduction of the temperature-sensitive SV40 large T-antigen (tsA58) and human telomerase reverse transcriptase (hTERT). Its defining feature is "conditional immortalization": cells proliferate continuously at 33°C, while at 37°-39°C, they cease division and exhibit a more mature, differentiated astrocyte phenotype. It is a premier tool for studying neuroinflammation, blood-brain barrier (BBB) dynamics, and astrocyte function in neurodegenerative diseases.

BioVector? HASTR/ci35 細(xì)胞系技術(shù)說明書 (Cell Line Datasheet)

中文版說明書

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? HASTR/ci35 人星形膠質(zhì)永生化細(xì)胞

• 細(xì)胞來源： 人類 (Human)

• 組織來源： 腦/星形膠質(zhì)細(xì)胞 (Brain/Astrocyte)

• 生長(zhǎng)特性： 貼壁生長(zhǎng)

• 細(xì)胞形態(tài)： 典型的星形或梭形

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? DMEM 培養(yǎng)基 或 BioVector? DMEM/F12 (1:1)

• 血清添加： 10% BioVector? 胎牛血清 (FBS)

• 培養(yǎng)環(huán)境： * 擴(kuò)增階段： 33°C，5% $CO_2$（激活大 T 抗原以促進(jìn)增殖）

  • 實(shí)驗(yàn)階段： 37°C - 39°C，5% $CO_2$（滅活大 T 抗原以誘導(dǎo)分化）

3. 細(xì)胞應(yīng)用

• 神經(jīng)毒性研究： 評(píng)估藥物或化學(xué)物質(zhì)對(duì)人類星形膠質(zhì)細(xì)胞的影響。

• 血腦屏障模型： 與內(nèi)皮細(xì)胞共培養(yǎng)構(gòu)建體外 BBB 模型。

• 病毒學(xué)研究： 作為研究嗜神經(jīng)病毒（如 HIV-1, Zika）感染的宿主細(xì)胞。

4. 注意事項(xiàng)

• 溫度敏感性： 嚴(yán)格控制孵育溫度。若需細(xì)胞增殖，切勿長(zhǎng)時(shí)間放置在 37°C 以上環(huán)境中。

• 標(biāo)記物表達(dá)： 細(xì)胞表達(dá)星形膠質(zhì)細(xì)胞標(biāo)志物，如膠質(zhì)纖維酸性蛋白 (GFAP) 和 S100B。建議在分化狀態(tài)下（37°C）檢測(cè)。

English Datasheet

1. General Product Information

• Product Name: BioVector? HASTR/ci35 Human Immortalized Astrocyte Cell Line

• Organism: Human

• Tissue Source: Brain / Astrocyte

• Growth Properties: Adherent

• Morphology: Typically stellate or spindle-shaped

2. Culture Conditions

• Basal Medium: BioVector? DMEM Medium or BioVector? DMEM/F12 (1:1)

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS)

• Incubation: * Expansion: 33°C, 5% $CO_2$ (Active Large T-antigen for proliferation)

  • Experimental/Differentiation: 37°C - 39°C, 5% $CO_2$ (Inactive Large T-antigen for maturation)

3. Applications

• Neurotoxicity Studies: Assessing the impact of drugs or chemicals on human astrocytes.

• BBB Modeling: Co-culture with endothelial cells to establish in vitro Blood-Brain Barrier models.

• Virology: Serving as a host cell for studying neurotropic viral infections (e.g., HIV-1, Zika).

4. Key Usage Notes

• Temperature Sensitivity: Precise temperature control is mandatory. To maintain proliferation, avoid prolonged exposure to temperatures above 37°C.

• Marker Profile: The cells express astrocyte markers such as Glial Fibrillary Acidic Protein (GFAP) and S100B. Marker expression is optimally analyzed under differentiated conditions (37°C).

Note: Handle BioVector? HASTR/ci35 cells under Biosafety Level 2 (BSL-2) conditions. To ensure the unique temperature-sensitive phenotype remains stable, always use BioVector? DMEM Medium and BioVector? Fetal Bovine Serum.

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