BioVector? pSAM-Bt 蘇云金芽孢桿菌穿梭表達(dá)質(zhì)粒載體 / BioVector? pSAM-Bt Plasmid Vector

通用定義 / General Definition:BioVector? pSAM-Bt 是一種專門用于蘇云金芽孢桿菌 (Bacillus thuringiensis, Bt) 的穿梭質(zhì)粒或表達(dá)載體。該載體通常包含在大腸桿菌 (E. coli) 和芽孢桿菌中均能發(fā)揮作用的復(fù)制起始位點(diǎn)，使其能夠在這兩種宿主之間進(jìn)行基因穿梭。它被廣泛應(yīng)用于 Bt 殺蟲晶體蛋白（Cry 蛋白）的異源表達(dá)、啟動(dòng)子功能表征以及針對(duì)農(nóng)業(yè)害蟲的基因工程菌株改良。

BioVector? pSAM-Bt is a specialized shuttle or expression vector designed for use in Bacillus thuringiensis (Bt). The vector typically contains origins of replication functional in both E. coli and Bacillus species, facilitating gene shuttling between these two hosts. It is widely used for the heterologous expression of Bt insecticidal crystal proteins (Cry proteins), promoter characterization, and the genetic engineering of Bt strains for agricultural pest control.

BioVector? pSAM-Bt 載體技術(shù)說明書 (Plasmid Datasheet)

中文版說明書

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? pSAM-Bt 表達(dá)載體

• 載體類型： 芽孢桿菌穿梭質(zhì)粒

• 常用宿主： 大腸桿菌 (E. coli)、蘇云金芽孢桿菌 (B. thuringiensis)

• 抗性基因： * 大腸桿菌： 氨芐青霉素 (Ampicillin) 或 紅霉素 (Erythromycin)

  • 芽孢桿菌： 紅霉素 (Erythromycin) 或 氯霉素 (Chloramphenicol)（視具體版本而定）

• 復(fù)制子： pMB1 (E. coli); pBT1 或類似 (Bt)

2. 培養(yǎng)與擴(kuò)增條件

• 大腸桿菌擴(kuò)增： 使用 BioVector? LB 培養(yǎng)基，添加相應(yīng)抗生素，37°C 培養(yǎng)。

• Bt 轉(zhuǎn)化與篩選： 使用 BioVector? GYS 或 LB 培養(yǎng)基，添加 BioVector? 紅霉素，通常在 28°C - 30°C 下培養(yǎng)以保持質(zhì)粒穩(wěn)定性。

• 誘導(dǎo)條件： 若帶有特定啟動(dòng)子，需根據(jù)其特性（如胞外蛋白分泌或芽孢形成期表達(dá)）進(jìn)行誘導(dǎo)。

3. 載體應(yīng)用

• Bt 基因工程： 用于表達(dá) Cry、Vip 等殺蟲蛋白，增強(qiáng)菌株的殺蟲譜。

• 啟動(dòng)子活性分析： 研究 Bt 芽孢形成階段特異性啟動(dòng)子的調(diào)控機(jī)制。

• 蛋白質(zhì)組學(xué)研究： 在 Bt 內(nèi)源環(huán)境下生產(chǎn)具有天然活性的蛋白質(zhì)。

4. 注意事項(xiàng)

• 質(zhì)粒穩(wěn)定性： 在 Bt 中培養(yǎng)時(shí)，由于質(zhì)粒拷貝數(shù)和環(huán)境壓力，建議在培養(yǎng)基中保持足量的 BioVector? 抗生素。

• 轉(zhuǎn)化方式： Bt 的轉(zhuǎn)化通常需要電穿孔法，建議使用 BioVector? Bt 電轉(zhuǎn)感受態(tài)細(xì)胞制備試劑盒。

English Datasheet

1. General Product Information

• Product Name: BioVector? pSAM-Bt Expression Vector

• Vector Type: Bacillus Shuttle Plasmid

• Common Hosts:E. coli, Bacillus thuringiensis (Bt)

• Antibiotic Resistance: * E. coli: Ampicillin or Erythromycin

  • Bacillus: Erythromycin or Chloramphenicol (version dependent)

• Origins of Replication: pMB1 (E. coli); pBT1 or equivalent (Bt)

2. Culture and Amplification Conditions

• E. coli Propagation: Use BioVector? LB Medium supplemented with appropriate antibiotics, incubated at 37°C.

• Bt Transformation & Selection: Use BioVector? GYS or LB Medium with BioVector? Erythromycin, typically incubated at 28°C - 30°C to ensure plasmid stability.

• Induction: If the vector contains specific promoters, follow the specific induction profile (e.g., stationary phase or sporulation-dependent expression).

3. Applications

• Bt Genetic Engineering: Expressing insecticidal proteins like Cry or Vip to expand the insecticidal spectrum of strains.

• Promoter Activity Assays: Studying the regulatory mechanisms of promoters specific to the Bt sporulation phase.

• Proteomics Research: Producing naturally active proteins within the endogenous Bt environment.

4. Key Usage Notes

• Plasmid Maintenance: To prevent plasmid loss in Bt, ensure consistent selection pressure using BioVector? Erythromycin in the growth media.

• Transformation Method: Electroporation is the standard method for Bt; using the BioVector? Bt Electro-competent Cell Preparation Kit is highly recommended.

Note: Handle all recombinant Bt strains according to local agricultural biosafety regulations. For high-yield plasmid prep, utilize the BioVector? Plasmid Midi Kit to ensure the purity required for sensitive electroporation procedures.

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

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