BioVector? pBHG-fiber5/35 腺病毒骨架載體 / BioVector? pBHG-fiber5/35 Adenoviral Backbone Plasmid

通用定義 / General Definition:

BioVector? pBHG-fiber5/35 是一種專(zhuān)門(mén)用于構(gòu)建嵌合型腺病毒系統(tǒng)的骨架質(zhì)粒。它基于 Ad5 腺病毒基因組，但關(guān)鍵性的 Fiber（纖維蛋白） 基因被改造為包含 Ad5 和 Ad35 的嵌合體（通常是 Ad5 的尾部結(jié)合 Ad35 的軸部和頭部）。這種設(shè)計(jì)改變了病毒的趨向性（Tropism），使其不再依賴(lài)于常規(guī)的 CAR 受體，而是通過(guò) CD46 受體感染細(xì)胞。這對(duì)于轉(zhuǎn)染 CAR 表達(dá)較低的細(xì)胞（如造血干細(xì)胞、免疫細(xì)胞和某些腫瘤細(xì)胞）至關(guān)重要。

BioVector? pBHG-fiber5/35 is an adenoviral backbone plasmid specialized for the construction of chimeric adenovirus systems. Based on the Ad5 genome, its key Fiber gene is engineered as a chimera containing components of both Ad5 and Ad35 (typically Ad5 tail and Ad35 shaft/knob). This modification shifts the viral tropism from the standard CAR receptor to the CD46 receptor. This is essential for transducing cells with low CAR expression, such as hematopoietic stem cells, immune cells, and certain malignant cells.

BioVector? pBHG-fiber5/35 載體技術(shù)說(shuō)明書(shū) (Plasmid Datasheet)

中文版說(shuō)明書(shū)

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱(chēng)： BioVector? pBHG-fiber5/35 骨架質(zhì)粒

• 病毒血清型： 嵌合型 Ad5/f35

• 受體趨向性： CD46 (人)

• 抗性基因： 氨芐青霉素 (Ampicillin) 或 結(jié)合特定體系的抗性

• 克隆方法： 與含有目的基因的 BioVector? 穿梭質(zhì)粒在 HEK293 細(xì)胞或大腸桿菌內(nèi)進(jìn)行重組。

2. 培養(yǎng)與擴(kuò)增條件

• 宿主菌： BioVector? Stbl3 或 DH5$\alpha$（建議使用高保真菌株以防止大質(zhì)粒重組）。

• 培養(yǎng)基： BioVector? LB 培養(yǎng)基

• 抗生素添加： BioVector? 氨芐青霉素 (100 $\mu$g/mL)

• 培養(yǎng)環(huán)境： 30°C - 37°C 恒溫振蕩培養(yǎng)（30°C 有助于維持大質(zhì)粒穩(wěn)定性）。

3. 載體應(yīng)用

• 難轉(zhuǎn)染細(xì)胞感染： 針對(duì) CAR 缺失的 T 細(xì)胞、B 細(xì)胞及干細(xì)胞的高效基因傳遞。

• 腺病毒包裝： 作為骨架載體，用于產(chǎn)生具有 5/35 嵌合特性的復(fù)制缺陷型腺病毒。

• 腫瘤靶向研究： 利用 CD46 在多種癌細(xì)胞中高表達(dá)的特性進(jìn)行靶向治療研究。

4. 注意事項(xiàng)

• 質(zhì)粒大?。?/strong> 該載體較大，提取時(shí)需使用 BioVector? 大質(zhì)粒提取試劑盒以確保完整性。

• 生物安全性： 包裝出的病毒具有感染人類(lèi)細(xì)胞的能力，必須在二級(jí)生物安全柜 (BSL-2) 中操作。

English Datasheet

1. General Product Information

• Product Name: BioVector? pBHG-fiber5/35 Backbone Plasmid

• Viral Serotype: Chimeric Ad5/f35

• Receptor Tropism: CD46 (Human)

• Antibiotic Resistance: Ampicillin (or specific to system)

• Cloning Method: Recombination with a BioVector? shuttle vector containing the gene of interest (GOI) in HEK293 cells or E. coli.

2. Culture and Amplification Conditions

• Host Strains: BioVector? Stbl3 or DH5$\alpha$ (High-fidelity strains recommended to prevent large plasmid rearrangement).

• Culture Medium: BioVector? LB Medium

• Antibiotic Supplement: BioVector? Ampicillin (100 $\mu$g/mL)

• Incubation: 30°C - 37°C constant shaking (30°C is preferred for maintaining stability of large constructs).

3. Applications

• Refractory Cell Transduction: High-efficiency gene delivery to CAR-deficient cells such as T-cells, B-cells, and stem cells.

• Adenovirus Packaging: Serves as the backbone for generating replication-deficient adenoviruses with 5/35 chimeric properties.

• Cancer Targeting: Exploiting the high expression of CD46 in various malignancies for targeted therapy research.

4. Key Usage Notes

• Plasmid Size: Due to its large size, use a BioVector? Large Plasmid Extraction Kit to ensure structural integrity.

• Biosafety: The resulting packaged virus is capable of infecting human cells; all downstream viral work must be conducted under Biosafety Level 2 (BSL-2) protocols.

Note: For maximum viral titer, ensure the purity of BioVector? pBHG-fiber5/35 before transfection. Use BioVector? Competent Cells for stable propagation of this complex backbone.

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

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