BioVector? CCSW1 人肝膽管癌細(xì)胞系CC-SW-1 / CCSW1 Human Intrahepatic Cholangiocarcinoma Cell Line

通用定義 / General Definition:

BioVector? CCSW1 是一種來源于人內(nèi)源性肝內(nèi)膽管癌（Intrahepatic Cholangiocarcinoma, iCCA）組織的人類細(xì)胞系。該細(xì)胞系保留了原發(fā)腫瘤的上皮特性和惡性生物學(xué)行為，是研究肝內(nèi)膽管癌發(fā)生機(jī)制、侵襲轉(zhuǎn)移能力以及開發(fā)靶向治療藥物的關(guān)鍵體外模型。它在分子水平上常用于探索膽管細(xì)胞的異常增殖調(diào)控及腫瘤微環(huán)境的相互作用。

BioVector? CCSW1 is a human cell line derived from intrahepatic cholangiocarcinoma (iCCA) tissue. This cell line retains the epithelial characteristics and malignant biological behavior of the primary tumor, serving as a critical in vitro model for studying the pathogenesis, invasion, and metastatic potential of intrahepatic bile duct cancer. At the molecular level, it is frequently used to explore abnormal proliferative regulation in cholangiocytes and interactions within the tumor microenvironment.

BioVector? CCSW1 細(xì)胞系技術(shù)說明書 (Cell Line Datasheet)

中文版說明書

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? CCSW1 人肝內(nèi)膽管癌細(xì)胞

• 細(xì)胞來源： 人類 (Human)

• 組織來源： 肝內(nèi)膽管 (Intrahepatic Bile Duct)

• 生長特性： 貼壁生長

• 細(xì)胞形態(tài)： 上皮細(xì)胞樣

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? DMEM 培養(yǎng)基 (或 BioVector? RPMI 1640 培養(yǎng)基)

• 血清添加： 10% BioVector? 胎牛血清 (FBS)

• 培養(yǎng)環(huán)境： 37°C，5% $CO_2$，飽和濕度

3. 細(xì)胞應(yīng)用

• 肝內(nèi)膽管癌的增殖、遷移與侵襲機(jī)制研究。

• 針對膽管癌的抗腫瘤藥物敏感性測試及篩選。

• 膽管癌相關(guān)信號(hào)通路（如 Notch, Wnt, MAPK）的研究。

4. 注意事項(xiàng)

• 細(xì)胞特性： BioVector? CCSW1 具有較強(qiáng)的成瘤能力，適用于建立異種移植 (CDX) 小鼠模型。

• 傳代建議： 當(dāng)細(xì)胞匯合度達(dá)到 80%-90% 時(shí)進(jìn)行傳代。由于其貼壁較牢，消化時(shí)需注意控制 BioVector? 胰酶的作用時(shí)間。

English Datasheet

1. General Product Information

• Product Name: BioVector? CCSW1 Human Intrahepatic Cholangiocarcinoma Cell Line

• Organism: Human

• Tissue Source: Intrahepatic Bile Duct

• Growth Properties: Adherent

• Morphology: Epithelial-like

2. Culture Conditions

• Basal Medium: BioVector? DMEM Medium (or BioVector? RPMI 1640 Medium)

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS)

• Incubation: 37°C, 5% $CO_2$, Saturated Humidity

3. Applications

• Research on proliferation, migration, and invasion mechanisms of intrahepatic cholangiocarcinoma.

• Anti-tumor drug sensitivity testing and screening specifically for bile duct cancer.

• Investigation of cholangiocarcinoma-related signaling pathways (e.g., Notch, Wnt, MAPK).

4. Key Usage Notes

• Tumorigenicity: BioVector? CCSW1 possesses robust tumorigenic potential, making it suitable for establishing cell line-derived xenograft (CDX) mouse models.

• Subculture: Perform subculture when cell density reaches 80%-90% confluence. Due to strong adherence, carefully monitor the incubation time with BioVector? Trypsin during dissociation.

Note: BioVector? CCSW1 cells must be handled under Biosafety Level 2 (BSL-2) conditions. To maintain the integrity of the malignant phenotype and experimental reproducibility, the use of BioVector? DMEM Medium and BioVector? Fetal Bovine Serum is highly recommended.

Supplier生產(chǎn)廠家:

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

E-mail: [email protected]

工作微信/QQ同號(hào)：1843439339

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