BioVector? mEER 小鼠口咽鱗狀細胞癌細胞系 / mEER Mouse Oropharyngeal Squamous Cell Carcinoma Cell Line

通用定義 / General Definition:

BioVector? mEER (Mouse Epithelial Endocervical Ras) 是一種經(jīng)由人乳頭瘤病毒 16 型 (HPV16) 的 E6 和 E7 癌蛋白以及活化的 H-Ras 基因穩(wěn)定轉(zhuǎn)染而成的 C57BL/6 小鼠細胞系。它是目前研究 HPV 陽性頭頸部鱗狀細胞癌 (HNSCC) 最具代表性的同系免疫模型。該細胞系不僅在遺傳背景上模擬了人類 HPV 相關的口咽癌，還能在免疫健全的小鼠體內(nèi)成瘤，廣泛用于腫瘤免疫治療和放療響應研究。

BioVector? mEER is a C57BL/6 mouse cell line stably transfected with Human Papillomavirus type 16 (HPV16) E6 and E7 oncoproteins, along with activated H-Ras. It is the gold-standard syngeneic model for studying HPV-positive Oropharyngeal Squamous Cell Carcinoma (OPSCC). By mimicking the genetic landscape of human HPV-related head and neck cancers, it enables the study of tumor growth in immunocompetent environments, making it essential for research in immunotherapy and radiotherapy responses.

BioVector? mEER 細胞系技術(shù)說明書 (Cell Line Datasheet)

中文版說明書

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? mEER 小鼠口咽鱗狀細胞癌細胞

• 細胞來源： 小鼠 (C57BL/6)

• 組織來源： 宮頸上皮（經(jīng)改造模擬口咽癌）

• 生長特性： 貼壁生長

• 細胞形態(tài)： 上皮細胞樣

2. 培養(yǎng)條件

• 基礎培養(yǎng)基： BioVector? DMEM/F12 培養(yǎng)基 (1:1)

• 血清添加： 10% BioVector? 胎牛血清 (FBS)

• 培養(yǎng)環(huán)境： 37°C，5% $CO_2$，飽和濕度

• 額外建議： 某些實驗方案可能需要添加特定生長因子（如胰島素或 $EGF$），視具體實驗目的而定。

3. 細胞應用

• HPV 陽性頭頸部鱗狀細胞癌 (HNSCC) 的發(fā)病機制研究。

• 評估針對 HPV 抗原的免疫檢查點抑制劑或癌癥疫苗。

• 腫瘤微環(huán)境中的免疫浸潤與逃逸機制研究。

4. 注意事項

• 生物安全性： 由于含有 HPV16 基因組片段，操作時需嚴格遵循生物安全 2 級 (BSL-2) 標準。

• 模型驗證： 在植入小鼠體內(nèi)前，建議通過 PCR 驗證 E6/E7 的表達以確保模型穩(wěn)定性。

English Datasheet

1. General Product Information

• Product Name: BioVector? mEER Mouse Oropharyngeal Squamous Cell Carcinoma Cell Line

• Organism: Mouse (C57BL/6)

• Tissue Source: Cervical Epithelium (Engineered to model OPSCC)

• Growth Properties: Adherent

• Morphology: Epithelial-like

2. Culture Conditions

• Basal Medium: BioVector? DMEM/F12 Medium (1:1)

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS)

• Incubation: 37°C, 5% $CO_2$, Saturated Humidity

• Additional Supplement: Specific protocols may require supplements like Insulin or $EGF$ depending on downstream applications.

3. Applications

• Pathogenesis studies of HPV-positive Head and Neck Squamous Cell Carcinoma (HNSCC).

• Evaluation of immune checkpoint inhibitors or cancer vaccines targeting HPV antigens.

• Research on immune infiltration and escape mechanisms within the tumor microenvironment.

4. Key Usage Notes

• Biosafety: As the cell line contains HPV16 E6/E7 sequences, it must be handled under Biosafety Level 2 (BSL-2) protocols.

• Validation: It is recommended to verify E6/E7 expression via PCR before in vivo implantation to ensure model consistency.

Note: For optimal results and to maintain the specific oncogenic expression profile, the use of BioVector? DMEM/F12 Medium and BioVector? Fetal Bovine Serum is highly recommended.

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