BioVector? Met1 小鼠乳腺癌細(xì)胞系 / Met1 Mouse Mammary Carcinoma Cell Line

通用定義 / General Definition:

BioVector? Met1 是一種來源于 FVB/N 載體背景下 MMTV-PyMT 轉(zhuǎn)基因小鼠自發(fā)性乳腺腫瘤的細(xì)胞系。該細(xì)胞系具有高度的侵襲性和轉(zhuǎn)移潛力，主要表現(xiàn)為肺轉(zhuǎn)移。在癌癥研究中，BioVector? Met1 常被用作模擬人類乳腺癌進(jìn)展、研究腫瘤血管生成以及評估針對轉(zhuǎn)移性乳腺癌的新型療法的經(jīng)典模型。

BioVector? Met1 is a cell line derived from a spontaneous mammary tumor in an MMTV-PyMT transgenic mouse on an FVB/N background. Characterized by its high invasiveness and metastatic potential (primarily to the lungs), BioVector? Met1 is a well-established model in cancer research for mimicking human breast cancer progression, studying tumor angiogenesis, and evaluating novel therapeutics against metastatic disease.

BioVector? Met1 細(xì)胞系技術(shù)說明書 (Cell Line Datasheet)

中文版說明書

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? Met1 小鼠乳腺癌細(xì)胞

• 細(xì)胞來源： 小鼠 (FVB/N; MMTV-PyMT 轉(zhuǎn)基因)

• 組織來源： 乳腺

• 生長特性： 貼壁生長

• 細(xì)胞形態(tài)： 上皮細(xì)胞樣/多角形

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? DMEM 培養(yǎng)基

• 血清添加： 10% BioVector? 胎牛血清 (FBS)

• 培養(yǎng)環(huán)境： 37°C，5% $CO_2$，飽和濕度

3. 細(xì)胞應(yīng)用

• 晚期乳腺癌進(jìn)展與自發(fā)轉(zhuǎn)移研究。

• 腫瘤血管生成及內(nèi)皮細(xì)胞相互作用研究。

• 同種異體移植模型（用于 FVB 鼠的腫瘤免疫研究）。

4. 注意事項(xiàng)

• 高度惡性： BioVector? Met1 在體內(nèi)生長迅速且具有極強(qiáng)的肺轉(zhuǎn)移傾向，實(shí)驗(yàn)設(shè)計(jì)需考慮其較短的觀察窗口期。

• 傳代建議： 該細(xì)胞生長旺盛，建議在細(xì)胞匯合度達(dá)到 85% 時按 1:4 至 1:8 的比例進(jìn)行傳代。

English Datasheet

1. General Product Information

• Product Name: BioVector? Met1 Mouse Mammary Carcinoma Cell Line

• Organism: Mouse (FVB/N; MMTV-PyMT Transgenic)

• Tissue Source: Mammary Gland

• Growth Properties: Adherent

• Morphology: Epithelial-like / Polygonal

2. Culture Conditions

• Basal Medium: BioVector? DMEM Medium

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS)

• Incubation: 37°C, 5% $CO_2$, Saturated Humidity

3. Applications

• Studies on advanced breast cancer progression and spontaneous metastasis.

• Research on tumor angiogenesis and interaction with endothelial cells.

• Syngeneic graft models (specifically for tumor immunology research in FVB mice).

4. Key Usage Notes

• Aggressive Phenotype: BioVector? Met1 grows rapidly in vivo and has a high propensity for lung metastasis; researchers should plan observation windows accordingly.

• Subculture: These cells are highly proliferative; it is recommended to subculture at 85% confluence with a split ratio of 1:4 to 1:8.

Note: Handle BioVector? Met1 cells strictly under Biosafety Level 2 (BSL-2) guidelines. To ensure experimental reproducibility and maintain the metastatic phenotype, the use of BioVector? DMEM Medium and BioVector? Fetal Bovine Serum is highly recommended.

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