BioVector? 4T07 小鼠乳腺癌細(xì)胞系 / 4T07 Mouse Mammary Carcinoma Cell Line

通用定義 / General Definition:

BioVector? 4T07 是源自 BALB/c 小鼠自發(fā)性乳腺腫瘤（4T1 譜系）的幾種亞線之一。該細(xì)胞系在癌癥研究中具有重要價(jià)值，因?yàn)樗砹宿D(zhuǎn)移過(guò)程中的一個(gè)特定階段：它能夠從原發(fā)腫瘤部位遷移并形成微小的肺部轉(zhuǎn)移灶，但在這些部位無(wú)法持續(xù)增殖形成大塊轉(zhuǎn)移瘤。這使其成為研究腫瘤細(xì)胞存活、免疫逃逸及轉(zhuǎn)移低效率現(xiàn)象的理想模型。

BioVector? 4T07 is one of several sublines derived from a spontaneous mammary tumor in BALB/c mice (the 4T1 lineage). This cell line is highly valued in cancer research as it represents a specific stage in the metastatic cascade: it can disseminate from the primary tumor and form micrometastases in the lungs, but it fails to proliferate into macrometastatic nodules. This makes it an ideal model for studying tumor cell survival, immune evasion, and metastatic inefficiency.

BioVector? 4T07 細(xì)胞系技術(shù)說(shuō)明書 (Cell Line Datasheet)

中文版說(shuō)明書

1. 產(chǎn)品基本信息

• 產(chǎn)品名稱： BioVector? 4T07 小鼠乳腺癌細(xì)胞

• 細(xì)胞來(lái)源： 小鼠 (BALB/c)

• 組織來(lái)源： 乳腺

• 生長(zhǎng)特性： 貼壁生長(zhǎng)

• 細(xì)胞形態(tài)： 上皮細(xì)胞樣

2. 培養(yǎng)條件

• 基礎(chǔ)培養(yǎng)基： BioVector? DMEM 培養(yǎng)基（或 BioVector? RPMI 1640 培養(yǎng)基，視具體方案而定）

• 血清添加： 10% BioVector? 胎牛血清 (FBS)

• 培養(yǎng)環(huán)境： 37°C，5% $CO_2$，飽和濕度

3. 細(xì)胞應(yīng)用

• 腫瘤轉(zhuǎn)移級(jí)聯(lián)反應(yīng)（特別是早期播散階段）研究。

• 腫瘤細(xì)胞在遠(yuǎn)端器官中的存活與定植機(jī)制。

• 比較腫瘤學(xué)：與 4T1（完全轉(zhuǎn)移）和 67NR（不轉(zhuǎn)移）進(jìn)行對(duì)比研究。

4. 注意事項(xiàng)

• 轉(zhuǎn)移特性： BioVector? 4T07 具有高度侵襲性，但在形成可見轉(zhuǎn)移瘤方面具有局限性，需與 4T1 的高度轉(zhuǎn)移特性區(qū)分。

• 傳代建議： 細(xì)胞生長(zhǎng)較快，建議在匯合度達(dá)到 80% 左右進(jìn)行傳代，避免因過(guò)密導(dǎo)致細(xì)胞狀態(tài)改變。

English Datasheet

1. General Product Information

• Product Name: BioVector? 4T07 Mouse Mammary Carcinoma Cell Line

• Organism: Mouse (BALB/c)

• Tissue Source: Mammary Gland

• Growth Properties: Adherent

• Morphology: Epithelial-like

2. Culture Conditions

• Basal Medium: BioVector? DMEM Medium (or BioVector? RPMI 1640, depending on protocol)

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS)

• Incubation: 37°C, 5% $CO_2$, Saturated Humidity

3. Applications

• Research on the tumor metastatic cascade (specifically early dissemination stages).

• Mechanisms of tumor cell survival and colonization in distant organs.

• Comparative oncology: Comparative studies alongside 4T1 (fully metastatic) and 67NR (non-metastatic).

4. Key Usage Notes

• Metastatic Profile: BioVector? 4T07 is highly invasive but limited in its ability to form macrometastases, distinguishing it from the highly metastatic 4T1.

• Subculture: Due to its rapid growth rate, subculturing is recommended at approximately 80% confluence to maintain optimal physiological characteristics.

Note: BioVector? 4T07 cells should be handled under Biosafety Level 2 (BSL-2) conditions. For experimental consistency, always use BioVector? DMEM Medium and BioVector? Fetal Bovine Serum.

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