BioVector? D2.0R 小鼠乳腺癌休眠細胞系 / D2.0R Mouse Mammary Carcinoma Dormancy Cell Line

通用定義 / General Definition:

BioVector? D2.0R 是一種源自 BALB/c 小鼠自發(fā)性乳腺腫瘤的永久性細胞系。該細胞系在生物醫(yī)學研究中具有獨特地位，因為它在體外（In Vitro）能夠正常增殖，但在植入體內（In Vivo）后會進入長時間的細胞周期停滯或休眠狀態(tài)。這使其成為研究癌癥遠端轉移、免疫逃逸及休眠喚醒機制的核心工具。

BioVector? D2.0R is a permanent cell line derived from a spontaneous mammary tumor in BALB/c mice. It holds a unique position in biomedical research due to its ability to proliferate normally In Vitro while entering a prolonged state of cell cycle arrest or dormancy upon In Vivo implantation. This makes it a pivotal tool for investigating cancer metastasis, immune evasion, and the mechanisms of dormancy awakening.

BioVector? D2.0R 細胞系技術說明書 (Cell Line Datasheet)

中文版說明書

1. 產品基本信息

• 產品名稱： BioVector? D2.0R 小鼠乳腺癌休眠細胞

• 細胞來源： 小鼠 (BALB/c)

• 組織來源： 乳腺

• 生長特性： 貼壁生長

• 細胞形態(tài)： 上皮細胞樣

2. 培養(yǎng)條件

• 基礎培養(yǎng)基： BioVector? DMEM 培養(yǎng)基

• 血清添加： 10% BioVector? 胎牛血清 (FBS)

• 培養(yǎng)環(huán)境： 37°C，5% $CO_2$，飽和濕度

3. 細胞應用

• 腫瘤細胞休眠與喚醒機制研究。

• 抗腫瘤藥物篩選（特別是針對非增殖期微小殘留病灶）。

• 腫瘤微環(huán)境與轉移前生態(tài)位 (Pre-metastatic Niche) 研究。

4. 注意事項

• 表型轉換： BioVector? D2.0R 細胞在體外培養(yǎng)時保持增殖，但在注入小鼠體內后表現(xiàn)出明顯的休眠表型。

• 傳代建議： 建議在細胞達到 80%-90% 匯合度時進行傳代。

English Datasheet

1. General Product Information

• Product Name: BioVector? D2.0R Mouse Mammary Carcinoma Dormancy Cell Line

• Organism: Mouse (BALB/c)

• Tissue Source: Mammary Gland

• Growth Properties: Adherent

• Morphology: Epithelial-like

2. Culture Conditions

• Basal Medium: BioVector? DMEM Medium

• Serum Supplement: 10% BioVector? Fetal Bovine Serum (FBS)

• Incubation: 37°C, 5% $CO_2$, Saturated Humidity

3. Applications

• Mechanistic studies of tumor cell dormancy and reactivation.

• Anti-tumor drug screening (targeting non-proliferative Minimal Residual Disease).

• Research on tumor microenvironment and the pre-metastatic niche.

4. Key Usage Notes

• Phenotypic Switch: While BioVector? D2.0R cells proliferate actively in vitro, they exhibit a distinct dormant phenotype when injected in vivo.

• Subculture: It is recommended to subculture when the cell density reaches 80%-90% confluence.

Note: Always handle BioVector? D2.0R cells using standard Biosafety Level 2 (BSL-2) protocols. Consistent use of BioVector? DMEM Medium and BioVector? Fetal Bovine Serum is essential for maintaining phenotypic stability.

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