BioVector? MC38-CEA Cell Line – Murine Colon Adenocarcinoma Cell Line Stably Expressing Human Carcinoembryonic Antigen

BioVector? MC38-CEA 細(xì)胞株 – 穩(wěn)定表達(dá)人癌胚抗原的小鼠結(jié)腸腺癌細(xì)胞株

1. Product Description / 產(chǎn)品描述

BioVector? MC38-CEA is a genetically engineered murine colon adenocarcinoma cell line derived from the parental MC38 line. It stably expresses human carcinoembryonic antigen (CEA, also known as CEACAM5) under a constitutive promoter. This cell line is widely used in immuno?oncology research, particularly for evaluating CEA?targeted therapies such as CAR?T cells, bispecific antibodies, antibody?drug conjugates (ADCs), and cancer vaccines in syngeneic mouse models with a functional immune system.

BioVector? MC38-CEA 是一款源自親本MC38細(xì)胞株的基因工程小鼠結(jié)腸腺癌細(xì)胞株。該細(xì)胞在組成型啟動(dòng)子驅(qū)動(dòng)下穩(wěn)定表達(dá)人癌胚抗原（CEA，又稱CEACAM5）。此細(xì)胞株廣泛用于腫瘤免疫學(xué)研究，尤其適用于在具有完整免疫功能的同源小鼠模型中評(píng)估靶向CEA的療法，如CAR?T細(xì)胞、雙特異性抗體、抗體藥物偶聯(lián)物（ADC）及癌癥疫苗。

2. Cell Line Specifications / 細(xì)胞株規(guī)格

3. Characterization Data / 鑒定數(shù)據(jù)

4. Applications / 應(yīng)用

• Evaluation of CEA?targeted immunotherapies (CAR?T cells, bispecific T?cell engagers, ADCs) in syngeneic C57BL/6 mouse models.

• Studying tumor immune microenvironment in CEA?expressing colon cancer.

• Screening and validation of CEA?specific antibodies and vaccines.

• Co?culture assays with human or mouse immune cells expressing CEA?directed receptors.

• 在同源C57BL/6小鼠模型中評(píng)估靶向CEA的免疫療法（CAR?T細(xì)胞、雙特異性T細(xì)胞銜接抗體、ADC）。

• 研究表達(dá)CEA的結(jié)腸癌腫瘤免疫微環(huán)境。

• 篩選與驗(yàn)證靶向CEA的特異性抗體及疫苗。

• 與表達(dá)CEA導(dǎo)向受體的人或小鼠免疫細(xì)胞進(jìn)行共培養(yǎng)實(shí)驗(yàn)。

5. Handling and Storage / 使用與儲(chǔ)存

• Shipping Format / 運(yùn)輸形式: Cryovial with >1×10? cells (frozen) or T?25 flask (70–80% confluent, ambient temperature for short distance)

• Immediate Handling / 接收后處理:

  • Frozen vial: Store in liquid nitrogen or –80?℃ (short term). Thaw rapidly in 37?℃ water bath and transfer to pre?warmed medium.

  • Live flask: Replace with fresh medium containing selection antibiotic after attachment (typically 4–6 hours).

• Subculturing / 傳代: Detach with 0.25% trypsin?EDTA when 80–90% confluent. Split ratio 1:5 to 1:10 twice per week.

• Freezing Medium / 凍存液: 90% FBS + 10% DMSO (or commercial serum?free freezing medium)

6. Quality Control / 質(zhì)量控制

• Sterility Testing: Negative for bacteria and fungi.

• Mycoplasma Detection: Negative (PCR or luminescence method).

• Identity Verification: Confirmed by flow cytometry for CEA expression and STR profiling for species/origin.

7. Citation / 引用說明

When using BioVector? MC38-CEA in your research, please cite the original publication(s) that established the line (if known) and include the following acknowledgement in your Materials and Methods:
“BioVector? MC38-CEA cell line (stable human CEA?expressing murine colon cancer cells) was used in this study (obtained from BioVector).”

在研究中引用BioVector? MC38-CEA時(shí)，請(qǐng)引用建立該細(xì)胞株的原始文獻(xiàn)（如已知），并在材料與方法部分注明：
“本研究使用了BioVector? MC38-CEA細(xì)胞株（穩(wěn)定表達(dá)人CEA的小鼠結(jié)腸癌細(xì)胞，由BioVector提供）。

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