NHEK BioVector? 正常人表皮角質(zhì)形成細(xì)胞 / NHEK BioVector? Normal Human Epidermal Keratinocytes

數(shù)據(jù)表 / Datasheet

細(xì)胞系信息

• 名稱：BioVector? NHEK 細(xì)胞系

• 詳細(xì)名稱：正常人表皮角質(zhì)形成細(xì)胞 NHEK（原代細(xì)胞）

• 來源：來源于人新生兒包皮或成人皮膚組織

• 細(xì)胞類型：原代角質(zhì)形成細(xì)胞

• 形態(tài)：鋪路石狀上皮細(xì)胞形態(tài)，貼壁生長，呈單層

• 特征：具有分化和增殖能力，表達(dá)角蛋白（K5、K14 等）；可用于研究表皮屏障功能、皮膚炎癥、傷口愈合及皮膚相關(guān)疾病

• 增殖能力：有限傳代次數(shù)（通常 3–6 代），之后進(jìn)入衰老期

• 病毒檢測(cè)：HIV-1-、HIV-2-、HBV-、HCV-、支原體陰性

• STR 鑒定：原代細(xì)胞不適用 STR 鑒定（為混合群體）

培養(yǎng)條件

• 培養(yǎng)基：專用角質(zhì)形成細(xì)胞無血清培養(yǎng)基（推薦 BioVector? Dermal Cell Basal Medium + 生長添加劑）或 BioVector? DMEM 添加 5% BioVector? 胎牛血清

• 添加劑：建議添加 BioVector? 重組人表皮生長因子（EGF）、BioVector? 牛垂體提取物（BPE）、BioVector? 氫化可的松

• 培養(yǎng)環(huán)境：37°C，5% CO?，飽和濕度

• 傳代方法：細(xì)胞達(dá)到 70–80% 匯合時(shí)傳代；使用 BioVector? 胰蛋白酶/EDTA 或?qū)Ｓ脗鞔噭┫?；?1:3 至 1:4 比例接種

• 凍存液：BioVector? 角質(zhì)形成細(xì)胞專用凍存液 或 BioVector? 胎牛血清含 10% BioVector? DMSO

• 生物安全等級(jí)：BSL-1（建議按 BSL-2 操作）

特征與用途

• 作為正常人表皮角質(zhì)形成細(xì)胞，是研究皮膚生物學(xué)、表皮分化、皮膚屏障功能、炎癥反應(yīng)及創(chuàng)面愈合的理想體外模型

• 廣泛用于皮膚毒理學(xué)測(cè)試（如刺激性、腐蝕性試驗(yàn)）及化妝品/藥物皮膚安全性評(píng)價(jià)

• 可構(gòu)建體外表皮模型（如重建人表皮）用于經(jīng)皮吸收、光老化及皮膚疾?。ㄣy屑病、特應(yīng)性皮炎）機(jī)制研究

• 用于病毒（如人乳頭瘤病毒 HPV）感染機(jī)制及疫苗研究

• 在基因治療、組織工程及再生醫(yī)學(xué)中作為種子細(xì)胞使用

Cell Line Information

• Name: BioVector? NHEK Cell Line

• Detailed Name: Normal Human Epidermal Keratinocytes NHEK (Primary Cells)

• Source: Derived from human neonatal foreskin or adult skin tissue

• Cell Type: Primary keratinocytes

• Morphology: Cobblestone epithelial morphology, adherent, growing in monolayer

• Characteristics: Capable of differentiation and proliferation; express keratins (K5, K14, etc.); suitable for studies on epidermal barrier function, skin inflammation, wound healing, and skin-related diseases

• Proliferative Capacity: Limited passages (typically 3–6 passages) before entering senescence

• Virus Testing: Negative for HIV-1, HIV-2, HBV, HCV; mycoplasma negative

• Authentication: STR authentication not applicable for primary cells (mixed population)

Culture Conditions

• Medium: Specialized keratinocyte serum-free medium (BioVector? Dermal Cell Basal Medium + growth supplements recommended) or BioVector? DMEM with 5% BioVector? fetal bovine serum

• Additives: BioVector? recombinant human epidermal growth factor (EGF), BioVector? bovine pituitary extract (BPE), BioVector? hydrocortisone recommended

• Culture Environment: 37°C, 5% CO?, humidified atmosphere

• Subculturing: Passage when cells reach 70–80% confluence; use BioVector? trypsin/EDTA or specialized detachment reagent; subculture at 1:3 to 1:4 ratio

• Cryopreservation: BioVector? keratinocyte-specific cryopreservation medium or BioVector? fetal bovine serum with 10% BioVector? DMSO

• Biosafety Level: BSL-1 (standard BSL-2 practices recommended)

Characteristics & Applications

• Serves as a normal human epidermal keratinocyte model for studying skin biology, epidermal differentiation, skin barrier function, inflammatory responses, and wound healing

• Widely used in skin toxicology testing (e.g., irritation, corrosion) and safety evaluation of cosmetics and pharmaceuticals

• Can be used to construct in vitro epidermal models (e.g., reconstructed human epidermis) for studies on percutaneous absorption, photoaging, and skin diseases such as psoriasis and atopic dermatitis

• Suitable for research on viral infection mechanisms (e.g., human papillomavirus HPV) and vaccine development

• Serves as a seed cell source in gene therapy, tissue engineering, and regenerative medicine

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

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