人 B 細(xì)胞前體白血病細(xì)胞系 YCUB-4 / Human B Cell Precursor Leukemia Cell Line YCUB-4

數(shù)據(jù)表 / Datasheet

細(xì)胞系信息

• 名稱：BioVector? YCUB-4 細(xì)胞系

• 詳細(xì)名稱：人 B 細(xì)胞前體白血病細(xì)胞系 YCUB-4

• 來(lái)源：來(lái)源于 B 細(xì)胞前體急性淋巴細(xì)胞白血?。˙CP-ALL）患者的外周血

• 細(xì)胞類型：B 細(xì)胞前體白血?。˙CP-ALL）

• 形態(tài)：小而圓的細(xì)胞，懸浮生長(zhǎng)，呈單細(xì)胞狀態(tài)或小簇狀

• 免疫表型：CD10+、CD19+、CD20+、CD34+、HLA-DR+、TdT+；CD3-、CD13-、CD33-

• 主要遺傳特征：攜帶 B 細(xì)胞前體白血病相關(guān)的特征性遺傳改變（具體融合基因類型需參考原始文獻(xiàn)）

• 病毒檢測(cè)：EBV-、HBV-、HCV-、HIV-1-、HIV-2-、HTLV-1/2-、MLV-

• 支原體檢測(cè)：PCR 檢測(cè)陰性

• STR 鑒定：通過 STR 分析驗(yàn)證，與參考數(shù)據(jù)庫(kù)匹配

培養(yǎng)條件

• 培養(yǎng)基：BioVector? RPMI 1640 培養(yǎng)基，添加 10% BioVector? 胎牛血清

• 添加劑：建議添加 2 mM BioVector? L-谷氨酰胺

• 培養(yǎng)環(huán)境：37°C，5% CO?，飽和濕度

• 傳代方法：初始接種密度為 2–3 × 10? 個(gè)細(xì)胞/mL；每 2–3 天按 1:2 至 1:4 比例傳代；維持細(xì)胞密度在 3–8 × 10? 個(gè)細(xì)胞/mL

• 凍存液：BioVector? 胎牛血清含 5–10% BioVector? DMSO

• 生物安全等級(jí)：BSL-1（建議按 BSL-2 操作）

特征與用途

• 作為 B 細(xì)胞前體急性淋巴細(xì)胞白血病的體外模型，廣泛用于研究白血病的發(fā)生、發(fā)展和治療反應(yīng)

• 該細(xì)胞系為 YCUB-4R 耐藥細(xì)胞系的親本細(xì)胞系，兩者構(gòu)成對(duì)比研究體系，可用于探究化療耐藥機(jī)制

• 適用于藥物敏感性測(cè)試、基因表達(dá)調(diào)控研究、信號(hào)通路分析及新型抗白血病藥物的篩選

• CD34 陽(yáng)性、CD20 陽(yáng)性表型使其適用于研究 B 細(xì)胞分化發(fā)育及靶向 CD20 治療的敏感性

• 可用于構(gòu)建白血病異種移植模型，開展體內(nèi)藥效學(xué)評(píng)價(jià)

Cell Line Information

• Name: BioVector? YCUB-4 Cell Line

• Detailed Name: Human B Cell Precursor Leukemia Cell Line YCUB-4

• Source: Derived from the peripheral blood of a patient with B-cell precursor acute lymphoblastic leukemia (BCP-ALL)

• Cell Type: B cell precursor leukemia (BCP-ALL)

• Morphology: Small, round cells growing in suspension, singly or in small clusters

• Immunophenotype: CD10+, CD19+, CD20+, CD34+, HLA-DR+, TdT+; CD3-, CD13-, CD33-

• Key Genetic Features: Carries characteristic genetic alterations associated with B-cell precursor leukemia (refer to original literature for specific fusion gene type)

• Virus Testing: Negative for EBV, HBV, HCV, HIV-1, HIV-2, HTLV-1/2, MLV

• Mycoplasma: Negative by PCR assay

• Authentication: Verified by STR analysis matching the reference database

Culture Conditions

• Medium: BioVector? RPMI 1640 supplemented with 10% BioVector? fetal bovine serum

• Additives: 2 mM BioVector? L-glutamine recommended

• Culture Environment: 37°C, 5% CO?, humidified atmosphere

• Subculturing: Seed at 2–3 × 10? cells/mL initially; split at 1:2 to 1:4 ratio every 2–3 days; maintain cell density between 3–8 × 10? cells/mL

• Cryopreservation: BioVector? fetal bovine serum with 5–10% BioVector? DMSO

• Biosafety Level: BSL-1 (standard BSL-2 practices recommended)

Characteristics & Applications

• Serves as an in vitro model for B-cell precursor acute lymphoblastic leukemia, widely used in studies of leukemogenesis, disease progression, and therapeutic response

• Functions as the parental cell line to YCUB-4R, establishing a comparative system for investigating mechanisms of chemotherapy resistance

• Suitable for drug sensitivity testing, gene expression regulation studies, signaling pathway analysis, and screening of novel anti-leukemic agents

• CD34-positive and CD20-positive immunophenotype makes it suitable for studying B-cell differentiation and sensitivity to CD20-targeted therapy

• Can be used to establish xenograft models for in vivo pharmacodynamic evaluation

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

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