人 B 細(xì)胞前體白血病細(xì)胞系 YCUB-6 / Human B Cell Precursor Leukemia Cell Line YCUB-6

數(shù)據(jù)表 / Datasheet

細(xì)胞系信息

• 名稱：BioVector? YCUB-6 細(xì)胞系

• 詳細(xì)名稱：人 B 細(xì)胞前體白血病細(xì)胞系 YCUB-6（攜帶 TCF3::PBX1 融合基因）

• 來源：2008 年建立，來源于一名 12 歲男孩復(fù)發(fā)急性淋巴細(xì)胞白血病（ALL）的外周血-1-2

• 細(xì)胞類型：B 細(xì)胞前體白血?。˙CP-ALL）-1-2

• 形態(tài)：小而圓的細(xì)胞，懸浮生長，呈單細(xì)胞狀態(tài)-1-2

• 免疫表型：CD3-、CD10+、CD13+、CD19+、CD20+、CD34-、CD37+、CD38+、CD80-、CD138+、HLA-DR+-1-2

• 主要遺傳特征：經(jīng) RT-PCR 驗證存在 TCF3::PBX1 融合基因（即 E2A::PBX1）-1-2

• 病毒檢測：EBV-、HBV-、HCV-、HIV-1-、HIV-2-、HTLV-1/2-、MLV--1

• 支原體檢測：PCR 檢測陰性-1-2

• STR 鑒定：通過 ANSI/ATCC ASN-0002.1-2021 標(biāo)準(zhǔn) STR 分析，STR 譜與參考數(shù)據(jù)庫匹配-1-2

培養(yǎng)條件

• 培養(yǎng)基：BioVector? RPMI 1640 培養(yǎng)基，添加 10% BioVector? 胎牛血清-1-2

• 培養(yǎng)環(huán)境：37°C，5% CO?，飽和濕度-1-2

• 傳代方法：初始接種密度為 3 × 10? 個細(xì)胞/mL；每周按 1:2 比例傳代；保持細(xì)胞密度在 5–8 × 10? 個細(xì)胞/mL（需保持高密度）-1-2

• 倍增時間：約 3–4 天-1-2

• 最大密度：約 6–8 × 10? 個細(xì)胞/mL-1-2

• 凍存液：70% BioVector? RPMI 1640 培養(yǎng)基、20% BioVector? 胎牛血清、10% BioVector? DMSO-1-2

• 生物安全等級：BSL-1-1-2

特征與用途

• 攜帶 TCF3::PBX1 融合基因，是研究該融合基因在 B 細(xì)胞前體急性淋巴細(xì)胞白血病中致病機制的重要體外模型-1-2

• 該細(xì)胞系來源于復(fù)發(fā)患兒，對化療藥物敏感性研究具有重要價值；研究表明，YCUB-6 對苯達(dá)莫司汀聯(lián)合克拉屈濱或阿糖胞苷具有協(xié)同至相加效應(yīng)，可用于復(fù)發(fā)/難治性 ALL 的挽救治療方案研究-5-6

• 在 NKX6-3 基因表達(dá)調(diào)控研究中，YCUB-6 作為 TCF3::PBX1 陽性細(xì)胞系被用于驗證該融合基因?qū)ο掠伟谢虻恼{(diào)控作用-4

• 免疫表型為 CD19+、CD20+、CD34-，CD34 陰性提示該細(xì)胞系代表較為成熟的 pre-B 階段，適用于 B 細(xì)胞分化及靶向 CD20 治療研究-1-2

Cell Line Information

• Name: BioVector? YCUB-6 Cell Line

• Detailed Name: Human B Cell Precursor Leukemia Cell Line YCUB-6 (carrying TCF3::PBX1 fusion)

• Source: Established in 2008 from the peripheral blood of a 12-year-old boy with acute lymphoblastic leukemia (ALL) at relapse-1-2

• Cell Type: B cell precursor leukemia (BCP-ALL)-1-2

• Morphology: Small, round cells growing singly in suspension-1-2

• Immunophenotype: CD3-, CD10+, CD13+, CD19+, CD20+, CD34-, CD37+, CD38+, CD80-, CD138+, HLA-DR+-1-2

• Key Genetic Features: TCF3::PBX1 fusion gene verified by RT-PCR-1-2

• Virus Testing: PCR negative for EBV, HBV, HCV, HIV-1, HIV-2, HTLV-1/2, MLV-1

• Mycoplasma: Negative by PCR assay-1-2

• Authentication: STR analysis according to ANSI/ATCC ASN-0002.1-2021 standard resulted in an authentic STR profile matching the reference database-1-2

Culture Conditions

• Medium: BioVector? RPMI 1640 supplemented with 10% BioVector? fetal bovine serum-1-2

• Culture Environment: 37°C, 5% CO?, humidified atmosphere-1-2

• Subculturing: Seed out initially at 3 × 10? cells/mL; split 1:2 once a week; maintain high cell density at 5–8 × 10? cells/mL-1-2

• Doubling Time: Approximately 3–4 days-1-2

• Maximum Density: Approximately 6–8 × 10? cells/mL-1-2

• Cryopreservation: Frozen with 70% BioVector? RPMI 1640, 20% BioVector? fetal bovine serum, 10% BioVector? DMSO-1-2

• Biosafety Level: BSL-1-1-2

Characteristics & Applications

• Carries the TCF3::PBX1 fusion gene; serves as a critical in vitro model for studying the pathogenic mechanisms of this fusion in B-cell precursor acute lymphoblastic leukemia-1-2

• Derived from a relapsed pediatric patient; valuable for chemotherapy sensitivity studies. Research has demonstrated that YCUB-6 exhibits synergistic-to-additive effects when treated with bendamustine combined with cladribine or cytarabine, supporting its use in salvage therapy research for relapsed/refractory ALL-5-6

• Used in gene expression regulation studies, including investigations of NKX6-3 as a downstream target of TCF3::PBX1-4

• Immunophenotype CD19+, CD20+, CD34- (CD34 negative indicates a more mature pre-B stage); suitable for B-cell differentiation studies and CD20-targeted therapy research

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

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