小鼠抗人 CD20 單克隆抗體雜交瘤細(xì)胞系 M4A1-M2B9 / Mouse Anti-Human CD20 Monoclonal Antibody Hybridoma Cell Line M4A1-M2B9

數(shù)據(jù)表 / Datasheet

細(xì)胞系信息

• 名稱：BioVector? M4A1-M2B9 細(xì)胞系

• 詳細(xì)名稱：小鼠抗人 CD20 單克隆抗體雜交瘤細(xì)胞系 M4A1-M2B9

• 來源：由小鼠 B 細(xì)胞與骨髓瘤細(xì)胞融合建立的雜交瘤細(xì)胞株，分泌抗人 CD20 單克隆抗體

• 免疫原：人 CD20 抗原

• 抗體亞型：IgG2a（κ輕鏈）

• 形態(tài)：懸浮或半貼壁生長，圓形或短梭形，多呈集落狀

• 主要特征：穩(wěn)定分泌高親和力抗人 CD20 單克隆抗體，廣泛用于 B 細(xì)胞淋巴瘤研究和靶向治療開發(fā)

培養(yǎng)條件

• 培養(yǎng)基：BioVector? RPMI 1640 培養(yǎng)基，添加 10–20% BioVector? 胎牛血清

• 添加劑：建議添加 2 mM BioVector? L-谷氨酰胺、1 mM BioVector? 丙酮酸鈉

• 培養(yǎng)環(huán)境：37°C，5% CO?，飽和濕度

• 傳代方法：每 2–3 天按 1:3 至 1:5 比例傳代；維持細(xì)胞密度在 2–5 × 10? 個(gè)細(xì)胞/mL

• 凍存液：BioVector? 胎牛血清含 5–10% BioVector? DMSO

• 生物安全等級(jí)：BSL-1（建議按 BSL-2 操作）

特征與用途

• 持續(xù)分泌抗人 CD20 單克隆抗體，可用于抗體純化、功能研究及診斷試劑開發(fā)

• 作為雜交瘤細(xì)胞模型，用于單克隆抗體生產(chǎn)優(yōu)化、基因工程改造及穩(wěn)定性研究

• 在 B 細(xì)胞相關(guān)疾病（如淋巴瘤、自身免疫?。┑陌邢蛑委熝芯恐芯哂兄匾獞?yīng)用價(jià)值

Cell Line Information

• Name: BioVector? M4A1-M2B9 Cell Line

• Detailed Name: Mouse Anti-Human CD20 Monoclonal Antibody Hybridoma Cell Line M4A1-M2B9

• Source: Hybridoma cell line established by fusion of mouse B cells with myeloma cells; secretes anti-human CD20 monoclonal antibody

• Immunogen: Human CD20 antigen

• Antibody Isotype: IgG2a (κ light chain)

• Morphology: Suspension or semi-adherent growth, round or short spindle-shaped, often in clusters

• Key Features: Stably secretes high-affinity anti-human CD20 monoclonal antibody; widely used in B-cell lymphoma research and targeted therapy development

Culture Conditions

• Medium: BioVector? RPMI 1640 supplemented with 10–20% BioVector? fetal bovine serum

• Additives: 2 mM BioVector? L-glutamine, 1 mM BioVector? sodium pyruvate recommended

• Culture Environment: 37°C, 5% CO?, humidified atmosphere

• Subculturing: Split at 1:3 to 1:5 ratio every 2–3 days; maintain cell density between 2–5 × 10? cells/mL

• Cryopreservation: BioVector? fetal bovine serum with 5–10% BioVector? DMSO

• Biosafety Level: BSL-1 (standard BSL-2 practices recommended)

Characteristics & Applications

• Continuously secretes anti-human CD20 monoclonal antibody; suitable for antibody purification, functional studies, and diagnostic reagent development

• Serves as a hybridoma model for monoclonal antibody production optimization, genetic engineering, and stability studies

• Valuable for targeted therapy research in B-cell-associated diseases such as lymphoma and autoimmune disorders

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

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