人 B 細(xì)胞前體白血病細(xì)胞系 BEL-1 / Human Pro-B Cell Leukemia Cell Line BEL-1

數(shù)據(jù)表 / Datasheet

細(xì)胞系信息

• 名稱：BioVector? BEL-1 細(xì)胞系

• 詳細(xì)名稱：人 B 細(xì)胞前體白血病細(xì)胞系 BEL-1（攜帶 t(4;11) 易位）

• 來源：41 歲女性 B 細(xì)胞急性淋巴細(xì)胞白血病（B-ALL）患者外周血

• 免疫表型：祖 B 細(xì)胞（Pro-B）

• 形態(tài)：懸浮生長(zhǎng)，圓形，單細(xì)胞或小簇狀

• 主要遺傳特征：攜帶 t(4;11)(q21;q23) 染色體易位，形成 KMT2A::AFF1（MLL::AF4）融合基因；表達(dá)異常 p27kip1 轉(zhuǎn)錄本

培養(yǎng)條件

• 培養(yǎng)基：BioVector? RPMI 1640 培養(yǎng)基，添加 10–20% BioVector? 胎牛血清

• 添加劑：建議添加 2 mM BioVector? L-谷氨酰胺

• 培養(yǎng)環(huán)境：37°C，5% CO?，飽和濕度

• 傳代方法：每 2–3 天按 1:2 至 1:3 比例傳代；維持細(xì)胞密度在 0.5–1.0 × 10? 個(gè)細(xì)胞/mL

• 凍存液：BioVector? 胎牛血清含 5–10% BioVector? DMSO

• 生物安全等級(jí)：BSL-1（建議按 BSL-2 操作）

特征與用途

• 廣泛用于研究 KMT2A 重排 在急性淋巴細(xì)胞白血病中的致病機(jī)制

• 可作為藥物篩選、基因表達(dá)調(diào)控及靶向治療研究的體外模型

• 在免疫分型、信號(hào)通路及表觀遺傳學(xué)研究中具有重要應(yīng)用價(jià)值

Cell Line Information

• Name: BioVector? BEL-1 Cell Line

• Detailed Name: Human Pro-B Cell Leukemia Cell Line BEL-1 (carrying t(4;11) translocation)

• Source: Peripheral blood from a 41-year-old female with B-cell acute lymphoblastic leukemia (B-ALL)

• Immunophenotype: Pro-B cell

• Morphology: Round cells growing in suspension, singly or in small clusters

• Key Genetic Features: Carries t(4;11)(q21;q23) translocation resulting in KMT2A::AFF1 (MLL::AF4) fusion; expresses an aberrant p27kip1 transcript

Culture Conditions

• Medium: BioVector? RPMI 1640 supplemented with 10–20% BioVector? fetal bovine serum

• Additives: 2 mM BioVector? L-glutamine is recommended

• Culture Environment: 37°C, 5% CO?, humidified atmosphere

• Subculturing: Split at 1:2 to 1:3 ratio every 2–3 days; maintain cell density between 0.5–1.0 × 10? cells/mL

• Cryopreservation: BioVector? fetal bovine serum with 5–10% BioVector? DMSO

• Biosafety Level: BSL-1 (standard BSL-2 practices recommended)

Characteristics & Applications

• Widely used to study the pathogenic mechanisms of KMT2A rearrangements in acute lymphoblastic leukemia

• Serves as an in vitro model for drug screening, gene expression regulation, and targeted therapy research

• Valuable for immunophenotyping, signaling pathway analysis, and epigenetic studies

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

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