BioVector? CCUG 58541 大腸桿菌菌株 / Escherichia coli Strain 數(shù)據(jù)說明書

產(chǎn)品名稱: BioVector? CCUG 58541 菌株
同義詞: BioVector? SMI Ec1027; BioVector? 8623
中文名稱: BioVector? CCUG 58541 大腸桿菌多重耐藥菌株
英文名稱: BioVector? CCUG 58541 Multi-Drug Resistant Escherichia coli Strain
產(chǎn)品類別: 多重耐藥菌株；臨床參考菌株
菌株類型: 大腸桿菌 (Escherichia coli)
序列分型: ST297 (Warwick) / ST666 (Pasteur)
保藏編號: CCUG 58541
保藏機構(gòu): 瑞典哥德堡大學(xué)菌種保藏中心 (Culture Collection, University of Gothenburg)
保藏信息: 由瑞典公共衛(wèi)生局 B. Olsson-Liljequist 于 2009 年 8 月 21 日保藏 -2
鑒定方法: API ID32E -2

耐藥表型:
對 β-內(nèi)酰胺類抗生素（包括廣譜頭孢菌素和碳青霉烯類）、氟喹諾酮類、氨基糖苷類、甲氧芐啶等多類抗菌藥物同時耐藥 -2

分子耐藥機制:

1. β-內(nèi)酰胺類耐藥:

   • 超廣譜 β-內(nèi)酰胺酶 (ESBL): 攜帶 CTX-M-14 β-內(nèi)酰胺酶基因，可水解頭孢噻肟、頭孢曲松等廣譜頭孢菌素 -2

   • 碳青霉烯酶: 攜帶 OXA-48 型碳青霉烯酶基因，可水解厄他培南、美羅培南等碳青霉烯類抗生素

   • 遺傳支持: OXA-48 基因位于 IncL 型質(zhì)粒上，可水平傳播

2. 氟喹諾酮類耐藥:

   • 對環(huán)丙沙星高度耐藥

   • 機制涉及靶位點突變（GyrA、ParC）及質(zhì)粒介導(dǎo)的喹諾酮耐藥基因

3. 氨基糖苷類耐藥:

   • 對氨基糖苷類藥物耐藥 -2

4. 其他耐藥:

   • 對甲氧芐啶耐藥 -2

抗生素最低抑菌濃度 (MIC):

生理生化特征:

1. 生長條件: 需氧菌，37°C 生長良好 -1-3

2. 氧耐受: 好氧 -1

3. API ID32E 生化譜: 515 115 515 155 515 515 115 551 115 111 11 10 -2

4. 酶活性:

   • β-半乳糖苷酶: 陽性 -4

   • β-葡萄糖醛酸酶: 陽性 -4

   • 賴氨酸脫羧酶: 陽性 -4

   • 鳥氨酸脫羧酶: 陽性 -4

   • 精氨酸雙水解酶: 陰性 -4

   • 脲酶: 陰性 -4

5. 代謝特征:

   • 吲哚產(chǎn)生: 陽性 -4

   • D-葡萄糖產(chǎn)酸: 陽性 -4

   • D-甘露醇產(chǎn)酸: 陽性 -4

   • 海藻糖產(chǎn)酸: 陽性 -4

   • L-阿拉伯糖產(chǎn)酸: 陽性 -4

   • 蔗糖產(chǎn)酸: 陰性 -4

   • 山梨醇產(chǎn)酸: 陰性 -4

特性特征:

1. 多重耐藥性: 同時對碳青霉烯類、廣譜頭孢菌素、氟喹諾酮類和氨基糖苷類等多種重要臨床抗生素耐藥 -2

2. OXA-48 陽性: 攜帶 OXA-48 型碳青霉烯酶，該酶是國際范圍內(nèi)流行的主要碳青霉烯酶類型之一

3. ESBL 陽性: 攜帶 CTX-M-14 型超廣譜 β-內(nèi)酰胺酶，可水解廣譜頭孢菌素 -2

4. 質(zhì)粒介導(dǎo)耐藥: OXA-48 基因位于可水平轉(zhuǎn)移的 IncL 型質(zhì)粒上，具有傳播風險

5. 高?？寺? 屬于 ST666 型，是 OXA-48 型大腸桿菌的常見流行克隆型

6. 臨床分級: 作為碳青霉烯類耐藥腸桿菌科細菌（CRE），被世界衛(wèi)生組織列為關(guān)鍵優(yōu)先級耐藥菌

研究應(yīng)用:

1. 碳青霉烯類耐藥機制研究

2. 超廣譜 β-內(nèi)酰胺酶與碳青霉烯酶共表達機制研究

3. 新型抗生素（頭孢他啶-阿維巴坦、美羅培南-韋博巴坦等）的敏感性評估

4. 質(zhì)粒介導(dǎo)耐藥基因水平傳播機制研究

5. 聯(lián)合用藥策略研究

6. 臨床微生物實驗室診斷方法學(xué)評價

7. 醫(yī)院感染控制與耐藥菌監(jiān)測研究

培養(yǎng)基: BioVector? 大腸桿菌專用培養(yǎng)基（推薦基礎(chǔ)成分：LB 培養(yǎng)基、Mueller-Hinton 瓊脂、胰蛋白胨大豆瓊脂或血瓊脂）
培養(yǎng)條件: 氣相：95% 空氣，5% 二氧化碳（可選）；溫度：35-37°C；好氧培養(yǎng) -1
保存方法: -80°C 甘油保存或液氮長期保存
凍存保護劑: 20% 甘油 或 10% DMSO
產(chǎn)品形式: 凍干粉 / 甘油菌 / 斜面培養(yǎng)物
生物安全等級: 2（需在二級生物安全柜內(nèi)操作）
質(zhì)量控制: 無菌檢測：陰性；活力檢測：復(fù)蘇后生長良好；耐藥表型驗證：符合描述
儲存與運輸: 4-10°C 冷藏運輸（短期），干冰或液氮運輸（長期）
相關(guān)菌株: BioVector? SMI Ec1027（同菌株不同編號）-2
僅供科研使用

English:
BioVector? CCUG 58541 Escherichia coli Strain Datasheet

Product Name: BioVector? CCUG 58541 Strain
Synonyms: BioVector? SMI Ec1027; BioVector? 8623
Chinese Name: BioVector? CCUG 58541 大腸桿菌多重耐藥菌株
English Name: BioVector? CCUG 58541 Multi-Drug Resistant Escherichia coli Strain
Product Category: Multi-Drug Resistant Strain; Clinical Reference Strain
Strain Type: Escherichia coli
Sequence Type: ST297 (Warwick) / ST666 (Pasteur)
Deposit Number: CCUG 58541
Depositor Institution: Culture Collection, University of Gothenburg, Sweden
Deposit Information: Deposited by B. Olsson-Liljequist, Public Health Agency of Sweden, on August 21, 2009 -2
Identification Method: API ID32E -2

Resistance Phenotype:
Resistant to multiple classes of antibiotics including β-lactams (including broad-spectrum cephalosporins and carbapenems), fluoroquinolones, aminoglycosides, and trimethoprim -2

Molecular Resistance Mechanisms:

1. β-Lactam Resistance:

   • Extended-Spectrum β-Lactamase (ESBL): Carries CTX-M-14 β-lactamase gene, hydrolyzing broad-spectrum cephalosporins such as cefotaxime and ceftriaxone -2

   • Carbapenemase: Carries OXA-48 carbapenemase gene, hydrolyzing carbapenems such as ertapenem and meropenem

   • Genetic Support: OXA-48 gene located on an IncL-type plasmid, capable of horizontal transfer

2. Fluoroquinolone Resistance:

   • Highly resistant to ciprofloxacin

   • Mechanisms involve target site mutations (GyrA, ParC) and plasmid-mediated quinolone resistance genes

3. Aminoglycoside Resistance:

   • Resistant to aminoglycosides -2

4. Other Resistance:

   • Resistant to trimethoprim -2

Minimum Inhibitory Concentrations (MICs):

Physiological and Biochemical Characteristics:

1. Growth Conditions: Aerobe, grows well at 37°C -1-3

2. Oxygen Tolerance: Aerobic -1

3. API ID32E Profile: 515 115 515 155 515 515 115 551 115 111 11 10 -2

4. Enzyme Activities:

   • β-Galactosidase: Positive -4

   • β-Glucuronidase: Positive -4

   • Lysine decarboxylase: Positive -4

   • Ornithine decarboxylase: Positive -4

   • Arginine dihydrolase: Negative -4

   • Urease: Negative -4

5. Metabolic Characteristics:

   • Indole production: Positive -4

   • D-Glucose acid production: Positive -4

   • D-Mannitol acid production: Positive -4

   • Trehalose acid production: Positive -4

   • L-Arabinose acid production: Positive -4

   • Sucrose acid production: Negative -4

   • Sorbitol acid production: Negative -4

Characteristics:

1. Multi-Drug Resistance: Simultaneously resistant to multiple important clinical antibiotics including carbapenems, broad-spectrum cephalosporins, fluoroquinolones, and aminoglycosides -2

2. OXA-48 Positive: Carries OXA-48 carbapenemase, one of the globally prevalent carbapenemase types

3. ESBL Positive: Carries CTX-M-14 extended-spectrum β-lactamase, hydrolyzing broad-spectrum cephalosporins -2

4. Plasmid-Mediated Resistance: OXA-48 gene located on a horizontally transferable IncL-type plasmid, posing transmission risks

5. High-Risk Clone: Belongs to ST666 type, a common epidemic clonal type for OXA-48-positive E. coli

6. Clinical Classification: Classified as a carbapenem-resistant Enterobacteriaceae (CRE), listed by WHO as a critical priority resistant pathogen

Research Applications:

1. Carbapenem resistance mechanism studies

2. Co-expression mechanisms of ESBL and carbapenemase

3. Susceptibility evaluation of novel antibiotics (ceftazidime-avibactam, meropenem-vaborbactam, etc.)

4. Horizontal transfer mechanism studies of plasmid-mediated resistance genes

5. Combination therapy strategy development

6. Clinical microbiology laboratory diagnostic method evaluation

7. Hospital infection control and resistant bacteria surveillance studies

Culture Medium: BioVector? E. coli Culture Medium (Recommended composition: LB medium, Mueller-Hinton agar, trypticase soy agar, or blood agar)
Culture Conditions: Atmosphere: 95% air, 5% CO? (optional); Temperature: 35-37°C; Aerobic -1
Storage Methods: -80°C glycerol stock or liquid nitrogen long-term storage
Cryoprotectant: 20% glycerol or 10% DMSO
Product Format: Freeze-dried powder / Glycerol stock / Slant culture
Biosafety Level: 2 (Operate in a Class II biological safety cabinet)
Quality Control: Sterility: Negative; Viability: Good growth upon revival; Resistance Phenotype Validation: Confirms description
Storage and Shipment: Refrigerated transport at 4-10°C (short-term), dry ice or liquid nitrogen transport (long-term)
Related Strain: BioVector? SMI Ec1027 (same strain under different designation) -2
For Research Use Only

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