BioVector? 銅綠假單胞菌 PAEAG1 菌株 / Pseudomonas aeruginosa PAEAG1 Strain 數(shù)據(jù)說(shuō)明書(shū)

產(chǎn)品名稱: BioVector? 銅綠假單胞菌 PAEAG1 菌株
同義詞: BioVector? P. aeruginosa AG1; BioVector? 多重耐藥銅綠假單胞菌菌株
中文名稱: BioVector? PAEAG1 多重耐藥銅綠假單胞菌菌株
英文名稱: BioVector? Pseudomonas aeruginosa PAEAG1 Multi-Drug Resistant Strain
產(chǎn)品類別: 多重耐藥菌株；高危克隆菌株
菌株類型: 銅綠假單胞菌 (Pseudomonas aeruginosa)
菌株分型: ST-111 高?？寺⌒?br />分離來(lái)源: 2010年分離自哥斯達(dá)黎加一家大型醫(yī)院患者的臨床樣本
保藏信息: 由哥斯達(dá)黎加大學(xué) Fernando García 課題組分離保藏
耐藥表型: 對(duì)β-內(nèi)酰胺類抗生素（包括碳青霉烯類）、氨基糖苷類和氟喹諾酮類等多類抗菌藥物同時(shí)耐藥

特性特征:

1. 雙重耐藥特征: 同時(shí)對(duì)β-內(nèi)酰胺類和氟喹諾酮類抗生素產(chǎn)生耐藥性，屬于多重耐藥（MDR）菌株-3

2. 金屬β-內(nèi)酰胺酶: 首次報(bào)道同時(shí)攜帶 VIM-2 和 IMP-18 基因編碼的金屬β-內(nèi)酰胺酶（MBL），這兩種酶均具有碳青霉烯酶活性，可水解碳青霉烯類抗生素-3

3. 基因組特征: 基因組包含 57 個(gè)基因組島，包括 6 個(gè)原噬菌體和 2 個(gè)完整的帶有 MBLs 基因的整合子；預(yù)測(cè)有 250 個(gè)基因與毒力相關(guān)，60 個(gè)基因與耐藥性相關(guān)-3

4. 氟喹諾酮耐藥機(jī)制: 環(huán)丙沙星處理后，518 個(gè)基因呈現(xiàn)差異表達(dá)，包括噬菌體基因上調(diào)-3

5. 臨床分級(jí): 由于對(duì)碳青霉烯類耐藥，被世界衛(wèi)生組織（WHO）列入耐藥菌優(yōu)先性列表的 Priority 1（關(guān)鍵優(yōu)先級(jí)）-3

6. 風(fēng)險(xiǎn)特征: 作為高?？寺。哂姓鞣t(yī)院環(huán)境的能力，可在醫(yī)療環(huán)境中持續(xù)存在并引起醫(yī)院感染-3

分子耐藥機(jī)制:

1. β-內(nèi)酰胺類耐藥:

   • 產(chǎn)金屬β-內(nèi)酰胺酶（VIM-2 和 IMP-18）-3

   • 染色體 AmpC 頭孢菌素酶高表達(dá)-2

   • 外膜孔蛋白 OprD 缺失導(dǎo)致碳青霉烯類耐藥-5

   • MexAB-OprM 等外排泵高表達(dá)-5

2. 氟喹諾酮類耐藥:

   • DNA 旋轉(zhuǎn)酶（GyrA）和拓?fù)洚悩?gòu)酶 IV（ParC）靶位點(diǎn)突變-2

   • 質(zhì)粒介導(dǎo)的喹諾酮耐藥（PMQR）基因（如 qnrA、qnrB、acc(6')-Ib-cr 等）-8

   • MexEF-OprN 外排泵高表達(dá)-5-6

3. 協(xié)同耐藥:

   • 同一菌株中多種耐藥機(jī)制共存，PMQR 與 ESBL 基因高度相關(guān)-8

   • 質(zhì)粒介導(dǎo)的耐藥基因可水平傳播-8

研究應(yīng)用:

1. 多重耐藥機(jī)制研究

2. 新型抗生素（如頭孢他啶-阿維巴坦、頭孢洛扎-他唑巴坦）的敏感性評(píng)估-5

3. 噬菌體療法研究-7

4. 耐藥性進(jìn)化與傳播機(jī)制研究

5. 高危克隆株的流行病學(xué)監(jiān)測(cè)

6. 聯(lián)合用藥策略研究

培養(yǎng)基: BioVector? 銅綠假單胞菌專用培養(yǎng)基（推薦基礎(chǔ)成分：LB 培養(yǎng)基、Mueller-Hinton 瓊脂或胰蛋白胨大豆瓊脂）
培養(yǎng)條件: 氣相：95%空氣，5%二氧化碳（可選）；溫度：35-37°C；好氧培養(yǎng)
保存方法: -80°C 甘油保存或液氮長(zhǎng)期保存
凍存保護(hù)劑: 20% 甘油 或 10% DMSO
產(chǎn)品形式: 凍干粉 / 甘油菌 / 斜面培養(yǎng)物
生物安全等級(jí): 2（需在二級(jí)生物安全柜內(nèi)操作）
質(zhì)量控制: 無(wú)菌檢測(cè)：陰性；活力檢測(cè)：復(fù)蘇后生長(zhǎng)良好；耐藥表型驗(yàn)證：符合描述
儲(chǔ)存與運(yùn)輸: 4-10°C 冷藏運(yùn)輸（短期），干冰或液氮運(yùn)輸（長(zhǎng)期）
僅供科研使用

English:
BioVector? Pseudomonas aeruginosa PAEAG1 Multi-Drug Resistant Strain Datasheet

Product Name: BioVector? Pseudomonas aeruginosa PAEAG1 Strain
Synonyms: BioVector? P. aeruginosa AG1; BioVector? Multi-Drug Resistant Pseudomonas aeruginosa Strain
Chinese Name: BioVector? PAEAG1 多重耐藥銅綠假單胞菌菌株
English Name: BioVector? Pseudomonas aeruginosa PAEAG1 Multi-Drug Resistant Strain
Product Category: Multi-Drug Resistant Strain; High-Risk Clone Strain
Strain Type: Pseudomonas aeruginosa
Strain Typing: ST-111 high-risk clonal type
Isolation Source: Isolated from clinical samples of patients at a major hospital in Costa Rica in 2010
Deposit Information: Isolated and deposited by the Fernando García Laboratory, University of Costa Rica
Resistance Phenotype: Resistant to multiple classes of antibiotics including beta-lactams (including carbapenems), aminoglycosides, and fluoroquinolones

Characteristics:

1. Dual Resistance Profile: Exhibits simultaneous resistance to beta-lactam and fluoroquinolone antibiotics; classified as a multi-drug resistant (MDR) strain-3

2. Metallo-beta-lactamases: First report of a P. aeruginosa isolate carrying both VIM-2 and IMP-18 genes encoding metallo-beta-lactamases (MBL), both with carbapenemase activity-3

3. Genomic Features: Genome contains 57 genomic islands, including 6 prophages and 2 complete integrons carrying MBL genes; 250 genes predicted to be associated with virulence and 60 genes associated with antibiotic resistance-3

4. Fluoroquinolone Resistance Mechanism: 518 genes show differential expression upon ciprofloxacin treatment, including upregulation of phage genes-3

5. Clinical Classification: Listed as Priority 1 (Critical Priority) by the World Health Organization (WHO) due to carbapenem resistance-3

6. Risk Characteristics: As a high-risk clone, capable of colonizing hospital environments and causing nosocomial infections-3

Molecular Resistance Mechanisms:

1. Beta-Lactam Resistance:

   • Production of metallo-beta-lactamases (VIM-2 and IMP-18)-3

   • Overexpression of chromosomal AmpC cephalosporinase-2

   • Loss of outer membrane porin OprD leading to carbapenem resistance-5

   • Overexpression of efflux pumps such as MexAB-OprM-5

2. Fluoroquinolone Resistance:

   • Target site mutations in DNA gyrase (GyrA) and topoisomerase IV (ParC)-2

   • Plasmid-mediated quinolone resistance (PMQR) genes (e.g., qnrA, qnrB, acc(6')-Ib-cr)-8

   • Overexpression of MexEF-OprN efflux pump-5-6

3. Synergistic Resistance:

   • Coexistence of multiple resistance mechanisms within the same strain; high correlation between PMQR and ESBL genes-8

   • Plasmid-mediated resistance genes can be horizontally transferred-8

Research Applications:

1. Multi-drug resistance mechanism studies

2. Susceptibility evaluation of novel antibiotics (e.g., ceftazidime-avibactam, ceftolozane-tazobactam)-5

3. Phage therapy research-7

4. Studies on resistance evolution and dissemination mechanisms

5. Epidemiological surveillance of high-risk clones

6. Combination therapy strategy development

Culture Medium: BioVector? Pseudomonas aeruginosa Culture Medium (Recommended composition: LB medium, Mueller-Hinton agar, or trypticase soy agar)
Culture Conditions: Atmosphere: 95% air, 5% CO? (optional); Temperature: 35-37°C; Aerobic
Storage Methods: -80°C glycerol stock or liquid nitrogen long-term storage
Cryoprotectant: 20% glycerol or 10% DMSO
Product Format: Freeze-dried powder / Glycerol stock / Slant culture
Biosafety Level: 2 (Operate in a Class II biological safety cabinet)
Quality Control: Sterility: Negative; Viability: Good growth upon revival; Resistance Phenotype Validation: Confirms description
Storage and Shipment: Refrigerated transport at 4-10°C (short-term), dry ice or liquid nitrogen transport (long-term)
For Research Use Only

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

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