BioVector? Vk12653 小鼠骨髓瘤細(xì)胞系 / Mouse Myeloma Cell Line 數(shù)據(jù)說(shuō)明書(shū)

產(chǎn)品名稱(chēng): BioVector? Vk12653 細(xì)胞
同義詞: BioVector? t-Vk12653; BioVector? Vk12653 骨髓瘤細(xì)胞
中文名稱(chēng): BioVector? Vk12653 小鼠骨髓瘤細(xì)胞系
英文名稱(chēng): BioVector? Vk12653 Mouse Myeloma Cell Line
產(chǎn)品類(lèi)別: 腫瘤細(xì)胞系；多發(fā)性骨髓瘤模型
物種來(lái)源: 小鼠 (Mus musculus)，C57BL/6 背景
組織來(lái)源: 脾臟 (從移植荷瘤小鼠脾臟分離)
細(xì)胞類(lèi)型: 漿細(xì)胞 (Plasma cell)
疾病: 多發(fā)性骨髓瘤 (Multiple Myeloma)
細(xì)胞標(biāo)志物: CD138+， B220-
構(gòu)建方法: 來(lái)源于 Vk*MYC 轉(zhuǎn)基因小鼠模型的自發(fā)性腫瘤，該小鼠攜帶受免疫球蛋白κ輕鏈基因增強(qiáng)子控制的 MYC 癌基因，可自發(fā)產(chǎn)生與人類(lèi)多發(fā)性骨髓瘤高度相似的漿細(xì)胞腫瘤。Vk12653 是經(jīng)過(guò)連續(xù)移植和硼替佐米選擇建立的移植性細(xì)胞系 (t-Vk12653)，通過(guò)連續(xù)傳代在 C57BL/6 小鼠中維持
形態(tài)特性: 漿細(xì)胞樣形態(tài)，懸浮生長(zhǎng)或輕微貼壁
生長(zhǎng)特性: 懸浮生長(zhǎng)
特性特征:

1. 分子分型: 根據(jù)轉(zhuǎn)錄組特征，Vk12653 屬于高風(fēng)險(xiǎn)分子分型中的 "Group A" 類(lèi)別，具有單染色體 5 單體性特征

2. 基因表達(dá)特征: 高表達(dá) EMC-92 高風(fēng)險(xiǎn)基因特征，但不表達(dá) UAMS-70 高風(fēng)險(xiǎn)基因特征；具有超增殖、髓外疾病特征

3. 耐藥特性: 對(duì)硼替佐米 (Bortezomib) 呈部分響應(yīng) (partial response)，對(duì)維奈托克 (Venetoclax) 具有不同程度的敏感性

4. 免疫特征: 誘導(dǎo) CD4 和 CD8 T 細(xì)胞耗竭

5. 移植模型: 可通過(guò)尾靜脈注射在免疫健全的 C57BL/6 小鼠中建立系統(tǒng)性多發(fā)性骨髓瘤模型，移植后在小鼠血清中可檢測(cè)到 M 蛋白 (單克隆免疫球蛋白)

6. 藥物響應(yīng): 在 LCL161 (IAP 拮抗劑) 和抗 CD137 激動(dòng)劑治療中顯示出響應(yīng)

7. 體外培養(yǎng): 在標(biāo)準(zhǔn)培養(yǎng)條件下難以長(zhǎng)期擴(kuò)增，需采用特殊培養(yǎng)條件 (如 3D 懸滴培養(yǎng)、低氧、高密度及細(xì)胞因子組合) 以維持細(xì)胞存活和功能

8. 基因修飾: 攜帶 MYC 轉(zhuǎn)基因，可通過(guò) Cre-loxP 系統(tǒng)調(diào)控
   研究應(yīng)用:

9. 多發(fā)性骨髓瘤發(fā)病機(jī)制研究

10. 高風(fēng)險(xiǎn)骨髓瘤分子分型研究

11. 耐藥機(jī)制研究 (硼替佐米部分響應(yīng)模型)

12. 免疫治療評(píng)估 (抗 CD137 激動(dòng)劑、IAP 拮抗劑等)

13. 聯(lián)合治療策略開(kāi)發(fā)

14. 腫瘤微環(huán)境與免疫細(xì)胞相互作用研究

15. 鐵代謝與骨髓瘤關(guān)系研究

16. 藥物篩選與療效評(píng)估
    培養(yǎng)基: BioVector? Vk12653 細(xì)胞專(zhuān)用完全培養(yǎng)基（推薦基礎(chǔ)成分：RPMI-1640 培養(yǎng)基，添加 10-20% 胎牛血清、1% 青霉素-鏈霉素、2mM L-谷氨酰胺、1mM 丙酮酸鈉，以及細(xì)胞因子組合如 IL-6 等）
    培養(yǎng)條件: 氣相：95% 空氣，5% 二氧化碳；溫度：37°C；推薦低氧培養(yǎng) (6.5% O?) 以增強(qiáng)細(xì)胞存活
    傳代方法: 細(xì)胞對(duì)培養(yǎng)條件敏感，常規(guī)傳代困難。推薦使用 BioVector? 專(zhuān)用培養(yǎng)體系，在 3D 懸滴或低氧條件下維持培養(yǎng)，每 2-3 天補(bǔ)充新鮮培養(yǎng)基
    凍存液: BioVector? 細(xì)胞凍存液（推薦配方：90% 胎牛血清 + 10% DMSO）
    質(zhì)量控制: 支原體檢測(cè)：陰性；無(wú)菌檢測(cè)：陰性；體內(nèi)成瘤能力驗(yàn)證：陽(yáng)性；M 蛋白檢測(cè)：陽(yáng)性
    儲(chǔ)存與運(yùn)輸: 液氮或干冰運(yùn)輸與保存
    生物安全等級(jí): 1（需在二級(jí)生物安全柜內(nèi)操作）
    僅供科研使用

English:
BioVector? Vk12653 Mouse Myeloma Cell Line Datasheet

Product Name: BioVector? Vk12653 Cells
Synonyms: BioVector? t-Vk12653; BioVector? Vk12653 Myeloma Cells
Chinese Name: BioVector? Vk12653 小鼠骨髓瘤細(xì)胞系
English Name: BioVector? Vk12653 Mouse Myeloma Cell Line
Product Category: Cancer Cell Line; Multiple Myeloma Model
Species of Origin: Mouse (Mus musculus), C57BL/6 background
Tissue of Origin: Spleen (isolated from spleens of transplanted tumor-bearing mice)
Cell Type: Plasma cell
Disease: Multiple Myeloma
Cell Markers: CD138+, B220-
Derivation: Derived from spontaneous tumors arising in the Vk*MYC transgenic mouse model, which carries the MYC oncogene under control of the immunoglobulin kappa light chain gene enhancer and spontaneously develops plasma cell tumors highly similar to human multiple myeloma. Vk12653 is a transplantable cell line (t-Vk12653) established through serial transplantation and bortezomib selection, maintained in C57BL/6 mice by serial passage
Morphology: Plasma cell-like morphology, suspension or weak adherent growth
Growth Characteristics: Suspension growth
Characteristics:

1. Molecular Subtype: Classified as "Group A" within high-risk molecular subtypes based on transcriptomic profiling, characterized by monosomy of chromosome 5

2. Gene Expression Profile: High expression of EMC-92 high-risk gene signature, but not UAMS-70 high-risk gene signature; features include hyperproliferation and extramedullary disease

3. Drug Resistance: Exhibits partial response to Bortezomib; variable sensitivity to Venetoclax

4. Immune Characteristics: Induces CD4 and CD8 T cell exhaustion

5. Transplant Model: Can establish systemic multiple myeloma in immunocompetent C57BL/6 mice via intravenous tail vein injection; M-protein (monoclonal immunoglobulin) detectable in mouse serum after transplantation

6. Drug Response: Shows response to LCL161 (IAP antagonist) and anti-CD137 agonist therapy

7. In Vitro Culture: Difficult to expand in standard culture conditions; requires specialized culture conditions (e.g., 3D hanging drop culture, hypoxia, high density, cytokine cocktail) to maintain cell viability and function

8. Genetic Modification: Carries MYC transgene that can be regulated by Cre-loxP system
   Research Applications:

9. Multiple myeloma pathogenesis studies

10. High-risk myeloma molecular subtyping research

11. Drug resistance mechanism studies (partial bortezomib response model)

12. Immunotherapy evaluation (anti-CD137 agonists, IAP antagonists, etc.)

13. Combination therapy strategy development

14. Tumor microenvironment and immune cell interaction studies

15. Iron metabolism and myeloma relationship studies

16. Drug screening and efficacy evaluation
    Culture Medium: BioVector? Complete Medium for Vk12653 Cells (Recommended composition: RPMI-1640 medium supplemented with 10-20% fetal bovine serum, 1% penicillin-streptomycin, 2mM L-glutamine, 1mM sodium pyruvate, and cytokine cocktail such as IL-6)
    Culture Conditions: Atmosphere: 95% air, 5% CO?; Temperature: 37°C; Hypoxic culture (6.5% O?) recommended to enhance cell survival
    Subculturing Method: Cells are sensitive to culture conditions; conventional passaging is difficult. Recommended to use BioVector? Specialized Culture System and maintain under 3D hanging drop or hypoxic conditions; replenish with fresh medium every 2-3 days
    Freeze Medium: BioVector? Cell Freeze Medium (Recommended composition: 90% FBS + 10% DMSO)
    Quality Control: Mycoplasma: Negative; Sterility: Negative; In Vivo Tumorigenicity Validation: Positive; M-protein Detection: Positive
    Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice
    Biosafety Level: 1 (Operate in a Class II biological safety cabinet)
    For Research Use Only

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