BioVector? Vk12598 小鼠骨髓瘤細(xì)胞系 / Mouse Myeloma Cell Line 數(shù)據(jù)說明書

產(chǎn)品名稱: BioVector? Vk12598 細(xì)胞
同義詞: BioVector? Vk12598 骨髓瘤細(xì)胞
中文名稱: BioVector? Vk12598 小鼠骨髓瘤細(xì)胞系
英文名稱: BioVector? Vk12598 Mouse Myeloma Cell Line
產(chǎn)品類別: 腫瘤細(xì)胞系；多發(fā)性骨髓瘤模型
物種來源: 小鼠 (Mus musculus)，C57BL/6 背景 -1-4
組織來源: 脾臟 (從移植荷瘤小鼠脾臟分離) -1-5
細(xì)胞類型: 漿細(xì)胞 (Plasma cell) -1
疾病: 多發(fā)性骨髓瘤 (Multiple Myeloma) -1-3
構(gòu)建方法: 來源于 Vk*MYC 轉(zhuǎn)基因小鼠模型的自發(fā)性腫瘤，該小鼠攜帶受免疫球蛋白κ輕鏈基因增強(qiáng)子控制的 MYC 癌基因，可自發(fā)產(chǎn)生與人類多發(fā)性骨髓瘤高度相似的漿細(xì)胞腫瘤。Vk12598 是其中一個可移植克隆，通過連續(xù)傳代在 C57BL/6 小鼠中維持 -5
形態(tài)特性: 漿細(xì)胞樣形態(tài)，懸浮生長或輕微貼壁
生長特性: 懸浮生長
細(xì)胞標(biāo)志物: CD138+， B220- -5
特性特征:

1. 耐藥特性: 對硼替佐米 (Bortezomib) 耐藥 -3-4

2. 移植模型: 可通過尾靜脈注射或股骨內(nèi)注射在免疫健全的 C57BL/6 小鼠中建立系統(tǒng)性多發(fā)性骨髓瘤模型 -2-7

3. 疾病特征: 移植后在小鼠血清中可檢測到 M 蛋白 (單克隆免疫球蛋白)，脾臟中可見 CD138+ 腫瘤細(xì)胞浸潤 -1-5

4. 體外培養(yǎng): 在標(biāo)準(zhǔn)培養(yǎng)條件下難以長期擴(kuò)增，需采用特殊培養(yǎng)條件（如 3D 懸滴培養(yǎng)、低氧、高密度及細(xì)胞因子組合）以維持細(xì)胞存活和功能 -5

5. 基因修飾: 攜帶 MYC 轉(zhuǎn)基因，可通過 Cre-loxP 系統(tǒng)調(diào)控 -1

6. 生物發(fā)光/熒光: 可根據(jù)實驗需要轉(zhuǎn)導(dǎo)熒光素酶或 GFP 基因，用于活體成像追蹤 -5-6

7. 研究應(yīng)用: 廣泛用于多發(fā)性骨髓瘤發(fā)病機(jī)制研究、耐藥機(jī)制研究、免疫治療評估、溶瘤病毒治療、聯(lián)合治療策略開發(fā)、骨病變研究、PET 示蹤劑評估等 -2-3-4
   培養(yǎng)基: BioVector? Vk12598 細(xì)胞專用完全培養(yǎng)基（推薦基礎(chǔ)成分：RPMI-1640 培養(yǎng)基，添加 10-20% 胎牛血清、1% 青霉素-鏈霉素、2mM L-谷氨酰胺、1mM 丙酮酸鈉，以及細(xì)胞因子組合如 IL-6 等）
   培養(yǎng)條件: 氣相：95% 空氣，5% 二氧化碳；溫度：37°C；推薦低氧培養(yǎng) (6.5% O?) 以增強(qiáng)細(xì)胞存活 -5
   傳代方法: 細(xì)胞對培養(yǎng)條件敏感，常規(guī)傳代困難。推薦使用 BioVector? 專用培養(yǎng)體系，在 3D 懸滴或低氧條件下維持培養(yǎng)，每 2-3 天補(bǔ)充新鮮培養(yǎng)基 -5
   凍存液: BioVector? 細(xì)胞凍存液（推薦配方：90% 胎牛血清 + 10% DMSO）
   質(zhì)量控制: 支原體檢測：陰性；無菌檢測：陰性；體內(nèi)成瘤能力驗證：陽性；M 蛋白檢測：陽性
   儲存與運輸: 液氮或干冰運輸與保存
   生物安全等級: 1（需在二級生物安全柜內(nèi)操作）
   僅供科研使用

English:
BioVector? Vk12598 Mouse Myeloma Cell Line Datasheet

Product Name: BioVector? Vk12598 Cells
Synonyms: BioVector? Vk12598 Myeloma Cells
Chinese Name: BioVector? Vk12598 小鼠骨髓瘤細(xì)胞系
English Name: BioVector? Vk12598 Mouse Myeloma Cell Line
Product Category: Cancer Cell Line; Multiple Myeloma Model
Species of Origin: Mouse (Mus musculus), C57BL/6 background -1-4
Tissue of Origin: Spleen (isolated from spleens of transplanted tumor-bearing mice) -1-5
Cell Type: Plasma cell -1
Disease: Multiple Myeloma -1-3
Derivation: Derived from spontaneous tumors arising in the Vk*MYC transgenic mouse model, which carries the MYC oncogene under control of the immunoglobulin kappa light chain gene enhancer and spontaneously develops plasma cell tumors highly similar to human multiple myeloma. Vk12598 is one transplantable clone maintained in C57BL/6 mice by serial passage -5
Morphology: Plasma cell-like morphology, suspension or weak adherent growth
Growth Characteristics: Suspension growth
Cell Markers: CD138+, B220- -5
Characteristics:

1. Drug Resistance: Resistant to Bortezomib -3-4

2. Transplant Model: Can establish systemic multiple myeloma in immunocompetent C57BL/6 mice via intravenous tail vein injection or intrafemoral injection -2-7

3. Disease Features: M-protein (monoclonal immunoglobulin) detectable in mouse serum after transplantation; CD138+ tumor cell infiltration observed in spleen -1-5

4. In Vitro Culture: Difficult to expand in standard culture conditions; requires specialized culture conditions (e.g., 3D hanging drop culture, hypoxia, high density, cytokine cocktail) to maintain cell viability and function -5

5. Genetic Modification: Carries MYC transgene that can be regulated by Cre-loxP system -1

6. Bioluminescence/Fluorescence: Can be transduced with luciferase or GFP genes for in vivo imaging tracking as needed -5-6

7. Research Applications: Widely used in multiple myeloma pathogenesis studies, drug resistance mechanism research, immunotherapy evaluation, oncolytic virus therapy, combination therapy strategy development, bone lesion studies, and PET tracer evaluation -2-3-4
   Culture Medium: BioVector? Complete Medium for Vk12598 Cells (Recommended composition: RPMI-1640 medium supplemented with 10-20% fetal bovine serum, 1% penicillin-streptomycin, 2mM L-glutamine, 1mM sodium pyruvate, and cytokine cocktail such as IL-6)
   Culture Conditions: Atmosphere: 95% air, 5% CO?; Temperature: 37°C; Hypoxic culture (6.5% O?) recommended to enhance cell survival -5
   Subculturing Method: Cells are sensitive to culture conditions; conventional passaging is difficult. Recommended to use BioVector? Specialized Culture System and maintain under 3D hanging drop or hypoxic conditions; replenish with fresh medium every 2-3 days -5
   Freeze Medium: BioVector? Cell Freeze Medium (Recommended composition: 90% FBS + 10% DMSO)
   Quality Control: Mycoplasma: Negative; Sterility: Negative; In Vivo Tumorigenicity Validation: Positive; M-protein Detection: Positive
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice
   Biosafety Level: 1 (Operate in a Class II biological safety cabinet)
   For Research Use Only

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