BioVector? EHSPEL 小鼠 parietal 內(nèi)胚層樣細(xì)胞系 / Mouse Parietal Endoderm-Like Cell Line 數(shù)據(jù)說明書

產(chǎn)品名稱: BioVector? EHSPEL 細(xì)胞
同義詞: BioVector? EHS Parietal Endoderm-Like; BioVector? Engelbreth-Holm-Swarm Parietal Endoderm-Like
中文名稱: BioVector? EHSPEL 小鼠 parietal 內(nèi)胚層樣細(xì)胞系
英文名稱: BioVector? EHSPEL Mouse Parietal Endoderm-Like Cell Line
產(chǎn)品類別: 腫瘤細(xì)胞系；基底膜基質(zhì)研究模型
物種來源: 小鼠 (Mus musculus)，ST/Eh 品系
組織來源: Engelbreth-Holm-Swarm 腫瘤
疾病: 小鼠軟骨肉瘤
細(xì)胞類型: parietal 內(nèi)胚層樣細(xì)胞
形態(tài)特性: 上皮樣細(xì)胞形態(tài)
生長(zhǎng)特性: 貼壁生長(zhǎng)
構(gòu)建方法: 從 Engelbreth-Holm-Swarm 腫瘤中分離建立，該腫瘤來源于 ST/Eh 品系小鼠的自發(fā)性腫瘤，通過移植傳代維持，本細(xì)胞系為可在體外培養(yǎng)的細(xì)胞系

特性特征:

1. 基底膜組分分泌: 細(xì)胞向培養(yǎng)基中分泌大量 laminin-1，并在培養(yǎng)皿上沉積基底膜組分

2. 體內(nèi)成瘤能力: 保留在體內(nèi)形成腫瘤的能力，且腫瘤可積累大量基底膜組分

3. 基因表達(dá)譜: 與胚胎第13.5天 Reichert's 膜的 parietal 內(nèi)胚層細(xì)胞、分化的 F9 胚胎癌細(xì)胞及 PYS-2 parietal 內(nèi)胚層細(xì)胞相比，具有共同的特征性基因表達(dá)模式，同時(shí)保留部分生殖細(xì)胞樣特征

4. 核型特征: 采用染色體涂染探針分析顯示存在大量染色體間重排及部分染色體超二倍體

5. 基因工程應(yīng)用: 可導(dǎo)入外源基因（如人 laminin-α4-EGFP 融合蛋白），產(chǎn)生并沉積人-鼠嵌合 laminin-8，適用于構(gòu)建嵌合 laminin 及具有修飾生物活性的基底膜樣凝膠的高效生產(chǎn)系統(tǒng)
   研究應(yīng)用:

6. 基底膜基質(zhì)工程與生產(chǎn)

7. 基底膜組分分泌機(jī)制研究

8. 嵌合 laminin 及修飾基底膜基質(zhì)的生產(chǎn)系統(tǒng)開發(fā)

9. 腫瘤微環(huán)境與基底膜相互作用研究
   培養(yǎng)基: BioVector? EHSPEL 細(xì)胞專用完全培養(yǎng)基（推薦基礎(chǔ)成分：DMEM 高糖培養(yǎng)基，添加10%胎牛血清和1%青霉素-鏈霉素）
   培養(yǎng)條件: 氣相：95%空氣，5%二氧化碳；溫度：37°C
   傳代方法: 當(dāng)細(xì)胞融合度達(dá)到80-90%時(shí)，推薦使用 BioVector? 專用傳代試劑（如0.25%胰蛋白酶-EDTA）進(jìn)行消化，按1:3至1:6比例進(jìn)行傳代
   凍存液: BioVector? 細(xì)胞凍存液（推薦配方：90%胎牛血清 + 10% DMSO）
   質(zhì)量控制: 支原體檢測(cè)：陰性；無菌檢測(cè)：陰性；基底膜組分分泌驗(yàn)證：陽(yáng)性
   儲(chǔ)存與運(yùn)輸: 液氮或干冰運(yùn)輸與保存
   生物安全等級(jí): 1
   僅供科研使用

English:
BioVector? EHSPEL Mouse Parietal Endoderm-Like Cell Line Datasheet

Product Name: BioVector? EHSPEL Cells
Synonyms: BioVector? EHS Parietal Endoderm-Like; BioVector? Engelbreth-Holm-Swarm Parietal Endoderm-Like
Chinese Name: BioVector? EHSPEL 小鼠 parietal 內(nèi)胚層樣細(xì)胞系
English Name: BioVector? EHSPEL Mouse Parietal Endoderm-Like Cell Line
Product Category: Cancer Cell Line; Basement Membrane Matrix Research Model
Species of Origin: Mouse (Mus musculus), ST/Eh strain
Tissue of Origin: Engelbreth-Holm-Swarm tumor
Disease: Mouse chondrosarcoma
Cell Type: Parietal endoderm-like cells
Morphology: Epithelial-like morphology
Growth Characteristics: Adherent
Derivation: Established from Engelbreth-Holm-Swarm tumor, which arose spontaneously in an ST/Eh strain mouse and has been propagated by transplantation. This cell line can be cultured ex vivo
Characteristics:

1. Basement Membrane Component Secretion: Secretes large amounts of laminin-1 into the culture medium and deposits basement membrane components onto dishes

2. In Vivo Tumor Formation: Retains the capacity to form tumors in vivo, with tumors accumulating abundant basement membrane components

3. Gene Expression Profile: Gene expression profile shares common features with parietal endoderm cells from Reichert's membrane at embryonic day 13.5, differentiated F9 embryonal carcinoma cells, and PYS-2 parietal endoderm cells, while also retaining germline cell-like characteristics

4. Karyotype: Chromosome painting analysis reveals a large number of interchromosomal rearrangements and partial chromosome hyperploidy

5. Genetic Engineering Applications: Can be used to introduce exogenous genes (e.g., human laminin-α4-EGFP fusion protein), resulting in production and deposition of human-mouse hybrid laminin-8; applicable for developing efficient systems to produce chimeric laminins and basement membrane-like gels with modified biological activity
   Research Applications:

6. Basement membrane matrix engineering and production

7. Mechanisms of basement membrane component secretion

8. Development of production systems for chimeric laminins and modified basement membrane matrices

9. Tumor microenvironment and basement membrane interaction studies
   Culture Medium: BioVector? Complete Medium for EHSPEL Cells (Recommended composition: DMEM high glucose medium supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin)
   Culture Conditions: Atmosphere: 95% air, 5% CO?; Temperature: 37°C
   Subculturing Method: When cells reach 80-90% confluence, recommended to use BioVector? Subculture Reagent (e.g., 0.25% Trypsin-EDTA). Subculture at a split ratio of 1:3 to 1:6
   Freeze Medium: BioVector? Cell Freeze Medium (Recommended composition: 90% FBS + 10% DMSO)
   Quality Control: Mycoplasma: Negative; Sterility: Negative; Basement Membrane Component Secretion Validation: Positive
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice
   Biosafety Level: 1
   For Research Use Only

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