BioVector? EndoC-βH1 人胰腺β細胞系 / Human Pancreatic Beta Cell Line 數(shù)據(jù)說明書

產(chǎn)品名稱: BioVector? EndoC-βH1 細胞
同義詞: BioVector? EndoC-β-H1; BioVector? EndoC?βH1
中文名稱: BioVector? EndoC-βH1 人胰腺β細胞系
英文名稱: BioVector? EndoC-βH1 Human Pancreatic Beta Cell Line
產(chǎn)品類別: 胰腺細胞系；條件永生化細胞系
物種來源: 人 (Homo sapiens)
組織來源: 胰腺；胰島
細胞類型: 胰腺β細胞
構建方法: 采用慢病毒轉(zhuǎn)導技術，將人端粒酶逆轉(zhuǎn)錄酶和SV40大T抗原導入從孕早期(8-10周)胎兒胰腺組織分離的胰島細胞，經(jīng)克隆篩選和條件永生化技術建立
永生化機制: 攜帶loxP位點包圍的永生化基因，可在Cre重組酶介導下切除，恢復細胞正常表型
形態(tài)特性: 上皮細胞樣；聚集生長形成胰島樣細胞團
生長特性: 貼壁生長；依賴細胞外基質(zhì)包被培養(yǎng)板
特性特征:

1. 胰島素分泌: 高表達人胰島素，葡萄糖刺激后胰島素分泌增加2-3倍；EC50約6.8mM葡萄糖

2. β細胞標志物: 表達成熟β細胞標志物，包括PDX1、NKX6.1、MAFA、GLUT2、GCK等

3. 胰島激素: 表達胰島素，不表達胰高血糖素、生長抑素、胰多肽

4. 轉(zhuǎn)錄組特征: 轉(zhuǎn)錄組譜與人類原代胰島β細胞高度相似

5. 分泌顆粒: 具有典型的β細胞分泌顆粒超微結構

6. 增殖特性: 需在含胰島素、轉(zhuǎn)鐵蛋白、硒、β-巰基乙醇等因子的專用培養(yǎng)基中培養(yǎng)

7. 研究應用: 1型和2型糖尿病發(fā)病機制研究、β細胞功能研究、胰島素分泌調(diào)控機制研究、藥物篩選、毒性測試、β細胞替代治療研究
   培養(yǎng)基: BioVector? EndoC-βH1 細胞專用完全培養(yǎng)基（推薦基礎成分：DMEM低糖培養(yǎng)基，添加2% BSA、50 μM β-巰基乙醇、10 μg/mL 轉(zhuǎn)鐵蛋白、6.7 ng/mL 亞硒酸鈉、100單位/mL 青霉素和100 μg/mL 鏈霉素）
   培養(yǎng)板包被: 培養(yǎng)板需預先用 BioVector? 細胞外基質(zhì)包被液（含1.5 μg/cm2 纖維連接蛋白和0.2 μg/cm2 膠原蛋白IV）在37°C包被1小時
   培養(yǎng)條件: 氣相：95%空氣，5%二氧化碳；溫度：37°C
   傳代方法: 當細胞融合度達到80-90%時，推薦使用 BioVector? 專用傳代試劑（如含EDTA的胰蛋白酶替代物）進行溫和消化，按1:2至1:4比例進行傳代；傳代后需在包被板上培養(yǎng)；每3-4天更換新鮮培養(yǎng)基
   凍存液: BioVector? 細胞凍存液（推薦配方：70%完全培養(yǎng)基 + 20%胎牛血清 + 10% DMSO）
   質(zhì)量控制: 支原體檢測：陰性；無菌檢測：陰性；胰島素分泌功能驗證：葡萄糖刺激響應陽性；β細胞標志物表達：陽性
   儲存與運輸: 液氮或干冰運輸與保存
   生物安全等級: 1（需在二級生物安全柜內(nèi)操作）
   僅供科研使用

English:
BioVector? EndoC-βH1 Human Pancreatic Beta Cell Line Datasheet

Product Name: BioVector? EndoC-βH1 Cells
Synonyms: BioVector? EndoC-β-H1; BioVector? EndoC?βH1
Chinese Name: BioVector? EndoC-βH1 人胰腺β細胞系
English Name: BioVector? EndoC-βH1 Human Pancreatic Beta Cell Line
Product Category: Pancreatic Cell Line; Conditionally Immortalized Cell Line
Species of Origin: Human (Homo sapiens)
Tissue of Origin: Pancreas; Islets of Langerhans
Cell Type: Pancreatic Beta Cell
Derivation: Established from islet cells isolated from first trimester (8-10 weeks) fetal pancreatic tissue by lentiviral transduction with human telomerase reverse transcriptase and SV40 large T antigen, followed by clonal selection and conditional immortalization technology
Immortalization Mechanism: Carries loxP-flanked immortalizing genes that can be excised by Cre recombinase to restore normal cell phenotype
Morphology: Epithelial-like; grows in clusters forming islet-like cell aggregates
Growth Characteristics: Adherent; requires extracellular matrix-coated culture plates
Characteristics:

1. Insulin Secretion: High expression of human insulin; glucose-stimulated insulin secretion increased 2-3 fold; EC50 approximately 6.8 mM glucose

2. Beta Cell Markers: Expresses mature beta cell markers including PDX1, NKX6.1, MAFA, GLUT2, GCK, etc.

3. Islet Hormones: Expresses insulin; does not express glucagon, somatostatin, or pancreatic polypeptide

4. Transcriptome Profile: Transcriptome profile highly similar to human primary islet beta cells

5. Secretory Granules: Possesses typical ultrastructure of beta cell secretory granules

6. Proliferation Characteristics: Requires specialized medium containing insulin, transferrin, selenium, β-mercaptoethanol, and other factors

7. Research Applications: Type 1 and Type 2 diabetes pathogenesis studies, beta cell function research, insulin secretion regulation mechanism studies, drug screening, toxicity testing, beta cell replacement therapy research
   Culture Medium: BioVector? Complete Medium for EndoC-βH1 Cells (Recommended composition: DMEM low glucose medium supplemented with 2% BSA, 50 μM β-mercaptoethanol, 10 μg/mL transferrin, 6.7 ng/mL sodium selenite, 100 units/mL penicillin, and 100 μg/mL streptomycin)
   Plate Coating: Culture plates must be pre-coated with BioVector? Extracellular Matrix Coating Solution (containing 1.5 μg/cm2 fibronectin and 0.2 μg/cm2 collagen IV) at 37°C for 1 hour
   Culture Conditions: Atmosphere: 95% air, 5% CO?; Temperature: 37°C
   Subculturing Method: When cells reach 80-90% confluence, recommended to use BioVector? Subculture Reagent (e.g., EDTA-containing trypsin substitute) for gentle dissociation. Subculture at a split ratio of 1:2 to 1:4. Culture on coated plates after subculture. Fresh medium renewal every 3-4 days
   Freeze Medium: BioVector? Cell Freeze Medium (Recommended composition: 70% complete medium + 20% FBS + 10% DMSO)
   Quality Control: Mycoplasma: Negative; Sterility: Negative; Insulin Secretion Function Validation: Glucose-stimulated response positive; Beta Cell Marker Expression: Positive
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice
   Biosafety Level: 1 (Operate in a Class II biological safety cabinet)
   For Research Use Only

Chinese:
BioVector? EndoC-βH5 人胰腺β細胞系 / Human Pancreatic Beta Cell Line 數(shù)據(jù)說明書

產(chǎn)品名稱: BioVector? EndoC-βH5 細胞
同義詞: BioVector? EndoC-β-H5; BioVector? EndoC?βH5
中文名稱: BioVector? EndoC-βH5 人胰腺β細胞系
英文名稱: BioVector? EndoC-βH5 Human Pancreatic Beta Cell Line
產(chǎn)品類別: 胰腺細胞系；條件永生化細胞系
物種來源: 人 (Homo sapiens)
組織來源: 胰腺；胰島
細胞類型: 胰腺β細胞
構建方法: 從EndoC-βH1細胞系基礎上進一步優(yōu)化構建，通過基因修飾增強β細胞功能和成熟度
永生化機制: 攜帶loxP位點包圍的永生化基因，可在Cre重組酶介導下切除，恢復細胞正常表型
形態(tài)特性: 上皮細胞樣；聚集生長形成胰島樣細胞團
生長特性: 貼壁生長；依賴細胞外基質(zhì)包被培養(yǎng)板
特性特征:

1. 胰島素分泌: 高表達人胰島素，葡萄糖刺激后胰島素分泌顯著增加；對葡萄糖刺激的敏感性高于EndoC-βH1細胞

2. β細胞標志物: 表達成熟β細胞標志物，包括PDX1、NKX6.1、MAFA、GLUT2、GCK等；表達水平高于EndoC-βH1

3. 成熟度: 細胞成熟度更高，更接近成人原代β細胞表型

4. 增殖特性: 需在含胰島素、轉(zhuǎn)鐵蛋白、硒、β-巰基乙醇等因子的專用培養(yǎng)基中培養(yǎng)

5. 研究應用: 1型和2型糖尿病發(fā)病機制研究、β細胞功能研究、胰島素分泌調(diào)控機制研究、藥物篩選、毒性測試、β細胞成熟機制研究、β細胞替代治療研究
   培養(yǎng)基: BioVector? EndoC-βH5 細胞專用完全培養(yǎng)基（推薦基礎成分：DMEM低糖培養(yǎng)基，添加2% BSA、50 μM β-巰基乙醇、10 μg/mL 轉(zhuǎn)鐵蛋白、6.7 ng/mL 亞硒酸鈉、100單位/mL 青霉素和100 μg/mL 鏈霉素）
   培養(yǎng)板包被: 培養(yǎng)板需預先用 BioVector? 細胞外基質(zhì)包被液（含1.5 μg/cm2 纖維連接蛋白和0.2 μg/cm2 膠原蛋白IV）在37°C包被1小時
   培養(yǎng)條件: 氣相：95%空氣，5%二氧化碳；溫度：37°C
   傳代方法: 當細胞融合度達到80-90%時，推薦使用 BioVector? 專用傳代試劑（如含EDTA的胰蛋白酶替代物）進行溫和消化，按1:2至1:4比例進行傳代；傳代后需在包被板上培養(yǎng)；每3-4天更換新鮮培養(yǎng)基
   凍存液: BioVector? 細胞凍存液（推薦配方：70%完全培養(yǎng)基 + 20%胎牛血清 + 10% DMSO）
   質(zhì)量控制: 支原體檢測：陰性；無菌檢測：陰性；胰島素分泌功能驗證：葡萄糖刺激響應陽性；β細胞標志物表達：陽性
   儲存與運輸: 液氮或干冰運輸與保存
   生物安全等級: 1（需在二級生物安全柜內(nèi)操作）
   僅供科研使用

English:
BioVector? EndoC-βH5 Human Pancreatic Beta Cell Line Datasheet

Product Name: BioVector? EndoC-βH5 Cells
Synonyms: BioVector? EndoC-β-H5; BioVector? EndoC?βH5
Chinese Name: BioVector? EndoC-βH5 人胰腺β細胞系
English Name: BioVector? EndoC-βH5 Human Pancreatic Beta Cell Line
Product Category: Pancreatic Cell Line; Conditionally Immortalized Cell Line
Species of Origin: Human (Homo sapiens)
Tissue of Origin: Pancreas; Islets of Langerhans
Cell Type: Pancreatic Beta Cell
Derivation: Optimized and constructed based on the EndoC-βH1 cell line, with enhanced beta cell function and maturity through genetic modifications
Immortalization Mechanism: Carries loxP-flanked immortalizing genes that can be excised by Cre recombinase to restore normal cell phenotype
Morphology: Epithelial-like; grows in clusters forming islet-like cell aggregates
Growth Characteristics: Adherent; requires extracellular matrix-coated culture plates
Characteristics:

1. Insulin Secretion: High expression of human insulin; significantly increased glucose-stimulated insulin secretion; higher sensitivity to glucose stimulation compared to EndoC-βH1 cells

2. Beta Cell Markers: Expresses mature beta cell markers including PDX1, NKX6.1, MAFA, GLUT2, GCK, etc.; expression levels higher than EndoC-βH1

3. Maturity: Higher degree of cellular maturity, more closely resembling adult primary beta cell phenotype

4. Proliferation Characteristics: Requires specialized medium containing insulin, transferrin, selenium, β-mercaptoethanol, and other factors

5. Research Applications: Type 1 and Type 2 diabetes pathogenesis studies, beta cell function research, insulin secretion regulation mechanism studies, drug screening, toxicity testing, beta cell maturation mechanism studies, beta cell replacement therapy research
   Culture Medium: BioVector? Complete Medium for EndoC-βH5 Cells (Recommended composition: DMEM low glucose medium supplemented with 2% BSA, 50 μM β-mercaptoethanol, 10 μg/mL transferrin, 6.7 ng/mL sodium selenite, 100 units/mL penicillin, and 100 μg/mL streptomycin)
   Plate Coating: Culture plates must be pre-coated with BioVector? Extracellular Matrix Coating Solution (containing 1.5 μg/cm2 fibronectin and 0.2 μg/cm2 collagen IV) at 37°C for 1 hour
   Culture Conditions: Atmosphere: 95% air, 5% CO?; Temperature: 37°C
   Subculturing Method: When cells reach 80-90% confluence, recommended to use BioVector? Subculture Reagent (e.g., EDTA-containing trypsin substitute) for gentle dissociation. Subculture at a split ratio of 1:2 to 1:4. Culture on coated plates after subculture. Fresh medium renewal every 3-4 days
   Freeze Medium: BioVector? Cell Freeze Medium (Recommended composition: 70% complete medium + 20% FBS + 10% DMSO)
   Quality Control: Mycoplasma: Negative; Sterility: Negative; Insulin Secretion Function Validation: Glucose-stimulated response positive; Beta Cell Marker Expression: Positive
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice
   Biosafety Level: 1 (Operate in a Class II biological safety cabinet)
   For Research Use Only

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